Banca de DEFESA: CLOVIS FERREIRA DOS REIS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : CLOVIS FERREIRA DOS REIS
DATA : 01/11/2019
HORA: 13:30
LOCAL: Sala B321, IMD
TÍTULO:

Systems Biology-Based Analysis of Transcriptional Data in Lead-Treated Human Neural Progenitor Cells

 

PALAVRAS-CHAVES:

lead (Pb) exposure, transcriptogramer, RNA-Seq, transcriptome analysis, time series, network inference, data integration, network visualization.

PÁGINAS: 108
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

The consequences of lead poisoning are diverse and relevant to human health. Reaching all organ systems, it mainly afects the nervous system, with severe and irreversible implicatons of neurodevelopment, memory consolidaton, and learning processes in children. They interact with cellular components in many ways, afectng ion-binding proteins, transducton signaling proteins, transmembrane ion channels, and transcripton factors. If in one hand, the symptoms of lead poisoning are well known, on the other hand, we have a lack of the systemic efects and its impact on neuronal cell transcripton modulaton. In order to investgate such efects from a systems biology perspectve, we applied the transcriptogramer R/Bioconductor package pipeline to evaluate the transcriptonal profle of lead acetate- treated human neural progenitor cells (NPCs) 30μM for 26 days. The transcriptogramer algorithm is designed to identfy functonally associated and diferentally expressed gene groups in case-control experiments in an unsupervised way. It was able to identfy eleven diferentally expressed clusters between days 3 and 11 of the lead treatment. Of these, seven presented negatve regulaton of several cellular systems involved in cell diferentaton, such as cytoskeleton organizaton, RNA and protein biosynthesis, characterized by large and tghtly connected networks. The four clusters that were positvely regulated presented sparse and poorly connected nodes, mainly related to transcripton, transmembrane transport, and signal transducton. In the subsequent period, involving days 12 to 26 of treatment, it was possible to observe a massive alteraton of the cellular transcripton profle with interference in all layers of gene expression regulaton. Thus, our results suggest that lead induces signifcant transcriptonal modifcatons in NPCs which can be correlated to damage and/or adaptatons of various systems, all resultng from intoxicaton by this heavy metal, thus influencing the result of ES-NP cell diferentaton.

MEMBROS DA BANCA:
Externa ao Programa - 1365498 - BEATRIZ STRANSKY FERREIRA
Externo à Instituição - DIEGO BONATTO - UFRGS
Externo à Instituição - MATHEUS AUGUSTO DE BITTENCOURT PASQUALI - UFCG
Presidente - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN
Externa ao Programa - 1674709 - VIVIANE SOUZA DO AMARAL