Banca de DEFESA: ANDRE LUIS FONSECA FAUSTINO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ANDRE LUIS FONSECA FAUSTINO
DATA : 01/11/2018
HORA: 09:00
LOCAL: IMD - Sala B205
TÍTULO:

Bioinformatics applied to oncology: Studies in the prospection of therapeutic targets, tumor antigens and in the dynamics of drug resistance.

PALAVRAS-CHAVES:

Cell surface proteins. Immunotherapy. Tumor antigens and drug resistance.

PÁGINAS: 56
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Cancer research is a field with several branches, which covers the understanding of how the tumor heterogeneity can be used as a treatment opportunity or how those alterations led poor prognosis and drug resistance. In this context, the bioinformatics rises as a tool to investigate which features could be used as a therapeutical strategy. In this thesis, we presented three chapters that address distinct aspects in the cancer research, such as i) the prospection of therapeutic targets, ii) identification of possible tumor antigens; iii) understanding mechanisms associated with drug resistance. In the first chapter, shown a catalog of cell surface proteins, herein called the surfaceome. The cell surface proteins represent attractive targets for therapy due to the essential role in signaling pathways and often dysregulation in cancer. The surfaceome catalog includes 3758 proteins, which were categorized based on genetic alterations types and the influence in short-term survival in several tumors. Furthermore, we investigate gene signatures and their association with survival rate. As result, three genes (WNT5A, CNGA2, and IGSF9B) were proposed as a poor prognosis in breast cancer patients. The second chapter, it is focused on data derived from a previous article, published in 2017. Briefly, the original publication was associated with the identification of cancer-testis antigens (CTAs) and relation with prognosis in several tumor types. On the other hand, in this chapter, we present new putative tumor antigens from a genome-wide analysis. Next, we discussed strategies to prioritize cases and remove spurious results. In addition, we purpose CTAs combinations as a strategy to increase the effectiveness in anticancer vaccines development. As result, were found significant combinations among HEATR9, INSL3, GTSF1L, and HSF5, which cover in average 35% of patients. Finally, the third chapter discusses a work in progress, which involves proteins associated with post-transcriptional regulation and how those proteins affect anticancer drug response. In particular, our findings suggest an interesting discussion about RBPs (RNA-Binding proteins) expression and response to anticancer drugs. Also, were compared RBPs findings with other transcriptional-related genes, such as transcriptional factors and lincRNAs. In conclusion, this thesis considers three fundamental aspects of cancer research, especially in the development of our treatment and diagnosis strategies. Furthermore, two of these chapters are supported by international publications.

MEMBROS DA BANCA:
Presidente - 1939184 - SANDRO JOSE DE SOUZA
Interno - 1267860 - GUSTAVO ANTONIO DE SOUZA
Interno - 1149647 - LUCYMARA FASSARELLA AGNEZ LIMA
Externo à Instituição - DIRCE MARIA CARRARO - ACCAMARGO
Externo à Instituição - VALDIR BALBINO - UFPE