Banca de DEFESA: LUCAS FELIPE DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUCAS FELIPE DA SILVA
DATA : 29/03/2018
HORA: 15:00
LOCAL: BioME
TÍTULO:

TFAT – TRANSCRIPTION FACTOR ANALYSIS TOOLS

PALAVRAS-CHAVES:

TFAT. Transcription Factors. Target genes. Degreeof reliability. Web tool. Enrichment analysis.

PÁGINAS: 49
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Currently there are several tools proposed for analysis of Transcription Factors (TF), such  as  TFCheckpoint,  JASPAR,  SSTAR,  GTRD,  Enrichr. However  none  of  these tools offers a complete experience in which the reliability of TF can be evaluated, that is,  if  in  fact  an  analyzed  protein  is  a  TF  and  its  association  with  the  target  gene. Numerous databases were built over time, all of them with very rich information, but the  intrinsic  complexity  of  the  data,  the  volume  of information,  problems  of  gene nomenclature  and  several  other  factors  meant  that  such  tools  did  not  offer  a complete spectrum of analysis . On the other hand,  to work with a large volume of data  requires  advanced  computer  skills.  However,  the  general  public  interested  in analyzing this data are professionals from the biological areas. Configuring itself as a barrier,  since  the  academic  formation  of  this  area  does  not  offer  in  its  curricular components  programming  disciplines.  Faced  with  this situation,  this  work  aims  to create  a  web  tool  exclusively  for  the  analysis  of TFs.  Containing  the  integration of different databases and a set of scripts to manipulate this information, along with the crucial parameters defined by the user in its analysis, Transcription Factor Analysis Tools (TFAT) was designed and developed. The core of this tool is the analysis to identify  the  key  TFs  in  the  modularization  of  gene  transcription,  that  is,  the enrichment of the regulatory TFs of a list of genessubmitted by the user, that through the  scripts  that  integrate  the  same,  consult  its  database,  identify  the  TFs  that  are associated  with  the  listed  genes  and  calculate  the  enrichment  p-value.  In  addition, the tool verifies TF reliability, makes available predictions, and converts items from a list to the Entrez Gene's GeneID or Symbol. Anotherfeature of this work is the use of TF reliability applied throughout the tool. This degree of reliability takes into account evidence from different databases, experiments, predictions and other characteristics of TFs. With a standard mode and a user-defined mode, this reliability feature allows for a full customization through filters in the queries and analysis control for the end user.

MEMBROS DA BANCA:
Presidente - 2170415 - JORGE ESTEFANO SANTANA DE SOUZA
Interno - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN
Externo à Instituição - WILFREDO BLANCO FIGUEROLA - UERN