Banca de QUALIFICAÇÃO: LUCAS FELIPE DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : LUCAS FELIPE DA SILVA
DATA : 28/02/2018
HORA: 09:00
LOCAL: BioME
TÍTULO:

TFAT – TRANSCRIPTION FACTOR ANALYSIS TOOLS

PALAVRAS-CHAVES:

TFAT.  Transcription Factors.  Target genes.

PÁGINAS: 60
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

There are currently several proposals for Transcription Factor analysis (TF), such as TFCheckpoint, JASPAR, SSTAR, GTRD, Enrichr. However, none of these tools offer a complete experience, where one can evaluate the reliability of TF, that is, if in fact one the analyzed protein is a TF and its association with the target gene. Over time, a large number of databases were built, all with very rich information, but the intrinsic complexity  of  the  data,  the  volume  of  information,  problems  of  gene  nomenclature and several other factors meant that such tools did not offer a complete spectrum of the  analysis.  On  the  other  hand,  to  work  with  a  large  volume  of  data  requires advanced  computer  skills.  However,  the  general  public  interested  in  analyzing  this data are professionals from the biological areas. Configuring itself as a barrier, since the  academic  formation  of  this  area  does  not  offer  in  its  curricular  component subjects of programming. Faced with this situation, this work aims to create a web tool  exclusively  for  the  analysis  of  TFs.  Containing  the  integration  of  different databases and a set of scripts to manipulate this information along with the crucial parameters  defined  by  the  user  in  his  analysis  was  designed  and  developed  the Transcription Factor Analysis Tools (TFAT). The core of this tool is the analysis to identify key TFs in the modularization of gene transcription, that is, the enrichment of the TFs regulators of a list of genes submitted by the user that through the scripts that  integrate  the  same  and  queries  in  its  database,  identify  the  TFs  that  are associated  with  the  list's  genes  and  calculates  p-value  and  methodology  used, among others. In addition, the tool verifies TF reliability, makes available predictions, and  converts  items  from  a  list  to  the  Entrez  Gene's  GeneID  or  Symbol.  Another feature of this work is the use of TF reliability app lied throughout the tool, this degree of  reliability  takes  into  account  evidence  in  different  databases,  experiments, predictions  and  other  characteristics  of  the  TF.  With  a  standard  mode  and  userdefined mode, this reliability feature allows for a full customization through filters in the queries and analysis control for the end user.

MEMBROS DA BANCA:
Presidente - 2170415 - JORGE ESTEFANO SANTANA DE SOUZA
Interno - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN