Banca de QUALIFICAÇÃO: FELIPHE LACERDA SOUZA DE ALENCAR

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : FELIPHE LACERDA SOUZA DE ALENCAR
DATA : 24/09/2019
HORA: 14:00
LOCAL: Sala de Reuniões, CB
TÍTULO:

BIOPROSPECTION AND PROTEOMICS OF Chromobacterium violaceum ISOLATED FROM AQUATIC ENVIRONMENTS IN BRAZILIAN SEMIARID: POTENTIALS FOR LEAD BIORMEDIATION

PALAVRAS-CHAVES:

Bioprospecting; Chromobacterium violaceum; Proteomics; Lead.

PÁGINAS: 341
GRANDE ÁREA: Outra
ÁREA: Ciências Ambientais
RESUMO:

The presence of toxic waste in the environment can cause harm not only to ecological balance, but also to human health. This problem intensifies when the perimeter of industries approaches to populated areas, especially those inserted in regions surrounding rivers, dams and reservoirs, such as the municipality of Lages Pintadas, located in the semi-arid region of Rio Grande do Norte, which suffers impacts from both anthropogenic action and natural radiation, with heavy metal lead as an important impact residue. Given the above, we present the need for the development and application of management tools and maintenance of environments impacted by lead, based on the study of the bioremediation potential of different strains of Chromobacterium violaceum. The isolation and microbiological identification of the respective bacterial strains occurred according to the methodology recommended by the Standard Methods for Examinations of Water and Wastewater. The strains were tested for the pattern of lead resistance at decreasing concentrations and different exposure times, and then evaluated by scanning electron microscopy for different bacterial arrangements against metal, followed by atomic absorption spectrophotometry evaluation of the metal removal levels tested in the presence of C. violaceum. Oxidative stress was based on the activity of the antioxidant enzyme catalase (CAT). After electrophoresis - SDS-PAGE, each lane was excised according to protein density. Protein sequencing was performed in a liquid chromatography system coupled to a mass spectrometer. The functional categories of the proteins were obtained with the Blast2GO program. Finally, to design the protein-protein interaction networks related to the lead response, the STITCH 3.1 tool was used. The data observed in this study are promising regarding the feasibility of using C. violaceum as a bioremediation tool to combat and mitigate the problematic pollution and environmental contamination by heavy metals. The C. violaceum resistance profiles at high metal concentrations indicate their metabolic versatility. However, despite the relevance of the application of this bacteria, there are gaps in scientific knowledge, given the existence of numerous hypothetical genes associated with heavy metal metabolism by C. violaceum.

MEMBROS DA BANCA:
Presidente - 1674709 - VIVIANE SOUZA DO AMARAL
Interno - 707.819.324-59 - JULIO ALEJANDRO NAVONI - UFRN
Externo ao Programa - 1880243 - DANIEL CARLOS FERREIRA LANZA
Externa à Instituição - NATALY ALBUQUERQUE DOS SANTOS - UFPB