Banca de QUALIFICAÇÃO: JENIELLY DE NORONHA FERREIRA DE CASTRO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JENIELLY DE NORONHA FERREIRA DE CASTRO
DATE: 20/10/2021
TIME: 13:00
LOCAL: Videoconferência - Link para acesso: meet.google.com/cfi-vgsv-apa
TITLE:

Biotechnological potential of bacteria isolated from consortiums from production water in oil reservoirs in Brazil

KEY WORDS:

Biodegradation of hydrocarbons, biosurfactants, production water, petroleum, bioinformatics.

PAGES: 130
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

During the extractive processes of oil and gas, production water is also formed, which is composed of organic and inorganic matter, in addition to microorganisms. The microbiota of this environment, adapted to extreme conditions, have great potential for application in biotechnological processes, such as bioremediation and/or MEOR. Within this context, this study aimed at the isolation, identification and characterization of bacteria from three previously established consortia obtained from production water. A total of 16 bacteria were isolated, fifteen of them selected for metabolic characterization from functional assays. The isolates were selected according to their ability to use hydrocarbons as a carbon source, for such growth curves were prepared using minimal medium supplemented with petroleum as the only carbon source. Considering the evaluation of hydrocarbon degradation, the test with DCPIP redox indicator was used and tests such as Emulsification Index, drop collapse and oil dispersion were applied to evaluate the production of biosurfactants/bioemulsifiers. The results obtained from the degradation tests confirmed the capacity of fifteen isolates to use petroleum and other hydrocarbons as the only source of carbon. The tests carried out for the production of biosurfactants confirmed the capacity of 15, among the sixteen isolates, to produce biosurfactants and/or to form an emulsion. Based on the 16S gene sequence, the isolates were identified as Bacillus, Acinetobacter, Staphylococcus, Ochrobactrum and Citrobacter. Among the isolates, four were selected and had their genomes sequenced, two of which were identified as Bacillus safensis, Ochrobactrum and Acinetobacter baumanii. Isolate AP1BH01-1, classified as a member of the genus Ochrobactrum, was designated as a new species, since it does not group with another species of the genus. The genomes of the sequenced isolates showed a great diversity of genes involved in the degradation of aliphatic and aromatic hydrocarbons, as well genes for the synthesis of biosurfactants and bioemulsifiers. Several genes related to resistance to heavy metals have also been identified. The data obtained through functional assays and genome mining of the isolates revealed promising profiles in hydrocarbon degradation processes and production of biosurfactants for application in bioremediation of environments contaminated with hydrocarbons and/or MEOR.

BANKING MEMBERS:
Externo ao Programa - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Externo ao Programa - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN
Externa à Instituição - ANA LÚCIA FIGUEIREDO PORTO - UFRPE