Banca de DEFESA: IZAEL DE SOUSA COSTA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : IZAEL DE SOUSA COSTA
DATE: 24/09/2021
TIME: 14:00
LOCAL: Videoconferência
TITLE:

BIOACTIVE VEGETABLE PROTEINS AND PEPTIDES AND THEIR EFFECTS ON GLYCEMIC CONTROL

KEY WORDS:

Hypoglycemic agents. Plant proteins. Signal transduction. Diabetes therapy. Vegetable proteins.

PAGES: 92
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Diabetes mellitus, type 2 (DM2) is a disease characterized by a state of blood hyperglycemia. Thus, the objective is to analyze the effect of plant-derived bioactive proteins on blood glucose control. This thesis was divided into three chapters, in the first and second there is the protocol and the systematic review (SR), aiming to answer the question: how isolated and purified proteins and peptides extracted from vegetables act in the reduction of blood glucose in experimental models of DM2? The protocol was registered in the International Prospective Registry of Systematic Reviews (PROSPERO) under number: CRD42019110956. For RS, experimental studies were selected with bioactive proteins and peptides of plant origin with an effect on DM2. Articles were selected according to the PICOS strategy (population, interventions, control, results and type of study) in the databases: PubMed, ScienceDirect, Scopus, Web of Science, EMBASE and Virtual Health Library, in March 2019. The initial search retrieved 916 articles and, after reading the title, abstract and keywords, 24 articles were eligible for full reading. After that, five articles were included in the RS. The assessment of evidence and strength of study recommendations was performed using the Systematic Review Center for Laboratory Animal Experimentation (SYRCLE) protocol. As a result of RS, it was found that all pathways stimulated by the five bioactive molecules of protein origin mediated secondary stimuli in the insulin signaling cascade up to glucose uptake. The third chapter presents a study with the trypsin inhibitor isolated from tamarind seed (ITT) and ITTp model number 56, and conformation number 287 (ITTp 56/287). In the preclinical study, the effect of ITT on the hyperglycemic state in Wistar rats with DM2 induced by a high glycemic index and high glycemic load (HGLI) diet for 17 weeks was evaluated. From the diagnosis of DM2 the animals (n = 15) were divided into three groups (n = 5): 1. DM2 without treatment; 2. DM2 treated with standard diet (nutritionally adequate); and 3. DM2 treated with ITT (25 mg/kg), administered by oral gavage (1 ml) for 10 days. After this period, fasting insulin and glycemia were analyzed, in addition to the Homeostasis model assessment for insulin resistance (HOMA-IR) and for β-cell function (HOMA-β). It was observed that the group of animals that received the treatment with the ITT, had lower fasting glucose concentration (p = 0.0031) and the HOMA-IR index (p = 0.0432), in addition to a higher HOMA-β index (p = 0.0052) when compared to the animals of the other groups evaluated. In the study by molecular dynamics simulation (DM) it was possible to observe interaction between the ITTp 56/287 and the insulin receptor (RI) (PDB ID 4OGA), this complex, ITTp 56/287-RI (-1591.54 kJ mol -1 ± 234.90), presented lower potential energy of interaction (EPI) compared to the Insulin-RI (Ins-RI) complex (-894.98 kJ mol-1 ± 32.16), with distinct amino acid residues involved in this interaction. With these studies, it can be seen that plant proteins, such as ITT, have antidiabetic potential as an adjuvant in hyperglycemic control by acting in metabolic pathways common to IR.

BANKING MEMBERS:
Externo à Instituição - EMMANUEL SILVA MARINHO - UECE
Externa ao Programa - 2323511 - ADRIANA AUGUSTO DE REZENDE
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externa à Instituição - JAILANE DE SOUZA AQUINO - UFPB
Externa ao Programa - 2306763 - KARINE CAVALCANTI MAURICIO DE SENA EVANGELISTA
Externo ao Programa - 2942606 - NORBERTO BATISTA DE FARIA JUNIOR