Banca de QUALIFICAÇÃO: VINÍCIUS CAMPELO SOEIRO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : VINÍCIUS CAMPELO SOEIRO
DATE: 20/07/2020
TIME: 14:00
LOCAL: Videoconferência
TITLE:

EVALUATION OF THE ANTIOXIDANT, ANTIBACTERIAL AND ANTIBIOFILM POTENTIAL OF SULFATED POLYSACARIDES OBTAINED FROM GREEN SEAWEED Codium isthmocladum

KEY WORDS:

Green seaweed, antioxidant, antibacterial, sulfated polysaccharide, purification

PAGES: 127
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

The green macroalgae Codium isthmocladum is a rich source of bioactive sulfated polysaccharides (SP). Besides being found in abundance in the Rio Grande do Norte coast, the SP from this alga have been already described about its anticoagulant and antinociceptive potential. On other hand, there are not any registers about the activities of this alga front of bacterial strains. In this context, the present study has as focus the extraction and purification of SP’s from the green alga C. isthmocladum, well as characterize them chemical and structurally. About its applicability, the SP’s were evaluated about the antioxidant capacity in many different in vitro ways, well has been evaluated the potential of this compounds as possible antibacterial and antibiofilm agents of normal and multiresistant bacteria. After being collected, the seaweed was treated with the purpose of obtaining a polysaccharide-rich isolate (crude extract). From a subsequent fractionation of this extract, five ketonic fractions were obtained (Ci 0.3, Ci 0.5, Ci 0.7, Ci 0.9, Ci 1.2). All fractions have a low content of proteins and phenolic compounds (> 1%). Ci 0.7 is the fraction with higher yield and sulfate content. All polysaccharides showed antioxidant capacity, but only Ci 0.7 and Ci 0.9 were able to inhibit the growth of resistant Staphylococcus aureus (MRSA) and Escherechia coli in adjuvant with several antibiotics, mainly with gentamicin. Both fractions were subjected to ion exchange chromatography and tended to generate two chromatographic peaks at different molarities, eluted with 2 M and 3 M NaCl, respectively for Ci 0.9 and 1 M and 2 M NaCl for Ci 0.7. Based on the data obtained from the chemical and structural analyzes, it is possible to affirm that these are sulfated homogalactans. The homogalactans from Ci-0.7-1M and Ci-0.9-3M presented antibacterial capacity and antibiofilm also against resistant strains of S. aureus

BANKING MEMBERS:
Presidente - 3060683 - LEONARDO THIAGO DUARTE BARRETO NOBRE
Externo à Instituição - GABRIEL PEREIRA FIDELIS
Externa à Instituição - KAROLINE RACHEL TEODOSIO DE MELO - FAMENE