Banca de QUALIFICAÇÃO: VANESSA CRISTINA OLIVEIRA DE LIMA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : VANESSA CRISTINA OLIVEIRA DE LIMA
DATE: 30/04/2020
TIME: 14:00
LOCAL: Videoconferência
TITLE:

Obesity induction with high glycemic index diet: effects on gene and hormone expression related to satiety signaling and safety in the use of tamarind trypsin inhibitor as an adjuvant in obesity treatment.

KEY WORDS:

Tamarindus indica; CCK; leptin; toxicity.

PAGES: 97
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:

Obesity is a multifactorial disease. In the search for adjuvants for classic lifestyle changes, travel inhibitors are prominent. In order to understand how these molecules can help in the treatment of obesity, a systematic review was carried out in this study. Twelve studies were found that involved the action of trypsin inhibitors in satiety in experimental models, whose main mechanism of action converges to the modulation of cholecystokinin (CCK). In addition, trypsin inhibitors can act on biochemical parameters related to these diseases, emerging as promising molecules. In general, experimental models of obesity aim to mimic a unbalanced poor dietary pattern. To understand how this pattern alters the hormonal balance that regulates energy metabolism, this study also evaluated the effect of a high glycemic index and glycemic load (HGLI) diet on body weight, plasma CCK and leptin and gene expression in the small intestine, trunk brain and visceral fat related to these hormones. Male Wistar rats (n = 5) were divided into two groups, one receiving the HGLI diet and the other standard diet, both ad libitum, for 17 weeks. The animals that received the HGLI diet increased the Body Mass Index (BMI) and the circulating leptin concentrations and their gene expression. There was no increase in plasma CCK, but the group that received the HGLI diet showed greater expression of CCK1R mRNA in the small intestine. The results suggest that leptin stimulated the expression of the CCK1R gene, regardless of the plasma increase in CCK, generating a synergistic action induced by the receptor, which can induce postprandial satiety. Analyzing the effects of diet on obesity enabled a better understanding of the role of trypsin inhibitors. The trypsin inhibitor of Tamarindo has been extensively studied in this model, showing several fronts of action, both in its isolated (ITT) and purified (ITTp) states. Due to the potential biological effect, there was a need to evaluate the safety of its use. ITT had already been analyzed from a toxicological point of view. Thus, in this study, the ITTp was evaluated for possible toxic effects, focusing on the organs involved in its metabolism (liver and pancreas) and the tissues affected by the obesity model (intestine and visceral adipose tissue). For this, Wistar rats (n = 5) were divided into a control group, fed a standard diet; obese group, fed with HGLI diet; experimental group, fed with HGLI and treated with ITTp. The treatment lasted 10 days. The tissues were analyzed (histopathological and stereological). It was observed that the administration of ITTp did not affect stereological parameters of the analyzed tissues, still acting as a protective factor of the intestinal mucosa, and that the histopathological changes were more consistent with the obesity model, leading to believe that ITTp, in addition to its biological activities, security in use.

BANKING MEMBERS:
Presidente - 1549705 - ADRIANA FERREIRA UCHOA
Externo à Instituição - ANDRE LUIZ SILVA DAVIM - UNI-RN
Interna - 2085604 - SUSANA MARGARIDA GOMES MOREIRA