Banca de QUALIFICAÇÃO: GÉSSICA LAIZE BERTO GOMES

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : GÉSSICA LAIZE BERTO GOMES
DATE: 24/04/2020
TIME: 09:00
LOCAL: videoconferencia
TITLE:

PROTEIN INTERACTION CHARACTERIZATION OF THE OF AUXIN REPRESSED PROTEIN (ARP) IN RESPONSE TO TOMATO FLOWERING CONTROL

KEY WORDS:

Solanum lycopersicum, Auxin Repressed Protein, Floral Transition, Two Hybrid, PHYTOEN SYNTHASE, CBS, Myf5, APE1 e GRX1.

PAGES: 119
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Biologia Molecular
SUMMARY:

Flowering is an important process for the plants and it corresponds to the transition from the vegetative to the reproductive phase. This transition is an important process that assure the reproductive success. Previous data from our laboratory using subtractive libraries identified a sequence that had homology to Auxin Repress Protein (ARP). This protein participates in the auxin signaling pathways and it regulates several important plant physiological processes. Therefore, the aim of this work was to understand the role of ARP protein in tomato and in flowering transition. First, it was done a sequence alignment using 13 protein sequences and omega clustal. In this alignment it was observed that the tomato ARP protein had the four domains that were characteristic from this family. The domain I is a leucine-rich motif, which has an amphiphilic repression region at its N-terminus. Domain II that contains an internal motif core composed of glycine, tryptophan and proline. Furthermore, at the C-terminal region, there are the domain III and IV, which together form the PB1 domain (Phox / Bem1p). This domain has a typical lysine and a series of acid residues. Using the STRING 10.0 database (http://www.string-dg.org) with a confidence index of 0.7, it was observed an interaction network where ARP1 protein interacts with more than 21 different proteins. The proteins CBS, Myf5, APE1 and GRX1 proteins, directly interact to ARP1 protein in this network. Moreover, these proteins are also expressed in tomato reproductive tissues as it was observed using the eFPBar tool (http://bar.utoronto.ca/efp_tomato/cgi -bin/efpWeb.cgi). In addition, using two hybrids tool, it was observed eight different protein-protein interactions in vivo. Three of these sequences codify two putative proteases and the third codify the CYTOCHROME C-OXIDASE protein. These proteins may be associated to cell energy metabolism. The other five sequences identified codify the PHYTOEN SYNTHASE protein. In order to better understand the possible role of ARP1 protein, then, it was used the Cytoscape 3.7.2 program in order to make an interactome network using the in silico and in vivo data. It was possible to identify the presence of five clusters, which are associated to plant energy metabolism, growth mechanism and cell differentiation. These pathways may be acting in the floral transition for tomato plants. Based on these results, it will be interesting to analyze using proteomic tools the tomato transgenic plants having the superexpression cassetes and it will be important to measure the auxin hormone level on these plants in order to understand the ARP1 protein role.

BANKING MEMBERS:
Presidente - 1549705 - ADRIANA FERREIRA UCHOA
Interno - 1228866 - EDUARDO LUIZ VOIGT
Externo à Instituição - CARLOS HENRIQUE SALVINO GADELHA MENESES - UEPB