Banca de QUALIFICAÇÃO: YAGO QUEIROZ DOS SANTOS
Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.STUDENT : YAGO QUEIROZ DOS SANTOS
DATE: 07/02/2020
TIME: 14:00
LOCAL: Sala Carl Peter von Dietrich - Departamento de Bioquímica
TITLE:
The current demand for renewable and green energy sources has spurred the search for alternatives that could replace the use of fossil fuels. One of the most promising innovations to positively impact the world energy scenario is the production of second generation bioethanol (2G-ethanol) from reducing sugars derived from enzymatic degradation of lignocellulosic material that is normally discarded in agroindustrial processes. Cellulose is the most abundant linear homopolysaccharide on the planet formed by glucose units linked by β- (1-4) glycosidic bonds. In addition to their primary structure formed by a sequence of glucose residues, cellulose microfibrils aggregate to form fibrils that are stabilized through the formation of various hydrogen bonds between the intra and interchain hydroxyl groups, a feature responsible for the crystalline structure. recalcitrant of this carbohydrate. The present work aimed the structural modeling as well as establishment of optimized enzymatic bioprocesses for the conversion of processed lignocellulosic material into fermentable reducing sugars. After the optimization process the maximum practical yield obtained was 318.809 ± 0.784 U/mL while in silico modeling showed the presence of monomeric enzymatic structure for the main endoglucanase involved in the process. According to the data obtained in this work, it was possible to conclude by the potential application of this optimized bioprocess in the industrial processes for bioethanol generation.
KEY WORDS:
Sugarcane bagasse-degrading bacteria; Thermostable enzymes; Bioprocessing.
PAGES: 102
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUMMARY:
The current demand for renewable and green energy sources has spurred the search for alternatives that could replace the use of fossil fuels. One of the most promising innovations to positively impact the world energy scenario is the production of second generation bioethanol (2G-ethanol) from reducing sugars derived from enzymatic degradation of lignocellulosic material that is normally discarded in agroindustrial processes. Cellulose is the most abundant linear homopolysaccharide on the planet formed by glucose units linked by β- (1-4) glycosidic bonds. In addition to their primary structure formed by a sequence of glucose residues, cellulose microfibrils aggregate to form fibrils that are stabilized through the formation of various hydrogen bonds between the intra and interchain hydroxyl groups, a feature responsible for the crystalline structure. recalcitrant of this carbohydrate. The present work aimed the structural modeling as well as establishment of optimized enzymatic bioprocesses for the conversion of processed lignocellulosic material into fermentable reducing sugars. After the optimization process the maximum practical yield obtained was 318.809 ± 0.784 U/mL while in silico modeling showed the presence of monomeric enzymatic structure for the main endoglucanase involved in the process. According to the data obtained in this work, it was possible to conclude by the potential application of this optimized bioprocess in the industrial processes for bioethanol generation.
BANKING MEMBERS:
Externo à Instituição - ANDERSON FELIPE JÁCOME DE FRANÇA
Presidente - 1267860 - GUSTAVO ANTONIO DE SOUZA
Externa à Instituição - LUCIANA MARIA ARAÚJO RABÊLO - IFRN