Banca de QUALIFICAÇÃO: MARIA TATIANA ALVES OLIVEIRA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : MARIA TATIANA ALVES OLIVEIRA
DATE: 26/11/2019
TIME: 14:00
LOCAL: Sala Carl Peter von Dietrich
TITLE:

ANTIMICROBIAL AND MODULADULATOR PROSPECTION OF GROSS EXTRACT AND FRACTIONS OF SEEDS OF Amburana cearensis

KEY WORDS:

Key-words: Amburana cearensis, Bacterial resistance, Candida spp., Phenolic compounds, Synergism.

PAGES: 113
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Química de Macromoléculas
SPECIALTY: Proteínas
SUMMARY:

Amburana cearensis (Fabaceae), popularly known as imburana or cumaru, its very used in traditional medicine, what awakens the search for evidence of its pharmacological properties. The aim of this study was to evaluate the antimicrobial and modulating activities of crude extract and fractions obtained from seeds of A. cearensis, as well as to characterize the plant material by UPLC-MS-QTOF. The aqueous extract obtained in 50 mM sodium phosphate buffer was sequentially fractionated into ammonium sulfate and subjected to characterization by the Acquity UPLC (Waters) system coupled to a Quadrupole/Flight Time (QtoF, Waters) system. The antimicrobial activity was verified by microdilution assay, while the possible interactions between the natural product and penicillin, norfloxacin, gentamicin and fluconazole were determined using a sub-inhibitory concentration. Samples at concentration of 128 µg/mL showed significant interaction with a possible synergistic effect when associated with antibiotics and antifungal against Escherichia coli, Sthaphylococcus aureus and Candida albicans. In the characterization it was possible to identify in the crude extract and fractions, several phenolic compounds, organic acids, and some polyphenols in positive ionization mode. The present study with crude extract and fractions of A. cearensis may be an alternative source of antimicrobial isolated compound.

 

 

BANKING MEMBERS:
Interno - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Interna - 1837354 - JULLIANE TAMARA ARAUJO DE MELO CAMPOS
Interna - 1453487 - KATIA CASTANHO SCORTECCI