Banca de DEFESA: YARA CAMPANELLI DE MORAIS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : YARA CAMPANELLI DE MORAIS
DATA : 30/09/2019
HORA: 09:00
LOCAL: Sala Carl Peter von Dietrich - Departamento de Bioquímica
TÍTULO:

EVALUATION OF IN VITRO AND IN VIVO EFFECT OF CHROMIUM PICOLINATE

PALAVRAS-CHAVES:

Genotoxicity. Cytotoxicity. Histological analysis. Metalomic analysis.

PÁGINAS: 72
GRANDE ÁREA: Ciências Biológicas
ÁREA: Biologia Geral
RESUMO:

Chromium picolinate (PICCr) is a complex made up of three picolinic acid residues (in salt form) linked by coordination to a chromium III atom. PICCr supplementation by athletes and others has been increasing every year and without a determined dose. The hypothesis of this study is the safety of prescribing varying doses of chromium picolinate. After commercial acquisition of PICCr capsules from two different countries, England and Brazil, this work began by checking by Atomic Absorption Spectrometry, the masses contained in these capsules. We found a higher concentration of the mineral in the capsules of English origin, which was selected as a sample for the research. Four cell lines: murine macrophages (RAW-264.7), Chinese hamster ovary (CHO) cells, murine fibroblast (3T3) and human human hepatic adecarcinoma (HepG2) cells were exposed to varying concentrations of PICCr (5 to 20 µg /mL) CHO, 3T3 and HepG2 were found to have their ability to reduce (10 to 80%) decreased MTT (3- (4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazole). RAW 264.7 cells were not affected in this analysis. HepG2 cells when exposed to PICCr (20 to 50 µg / mL) had micronucleus numbers significantly similar to cells that were not exposed to PICCr. RAW 264.7 cells showed a decrease in nitric oxide release when they were exposed to bacterial wall liposaccharides (LPS). In in vivo assays, male Swiss mice were exposed to different doses of PICCr (1000 and 2000 µg / mL) for 15 days. After this period, it was found that the treated animals had lower weight gain and decreased plasma cholesterol, triglycerides and glucose levels. They did not present macroscopic or histological changes in the hepatic and renal tissues. Treated animals were also found to have 50% decrease in serum plasma iron levels. However, metallomics analyzes show that iron and hepatic tissue iron levels have not been affected, however, human studies are necessary to confirm these observations, and may boost future studies to better understand these effects and add economic and pharmacological value to the study. PICCr.

MEMBROS DA BANCA:
Presidente - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Externa à Instituição - JAILMA ALMEIDA DE LIMA - CRI
Interna - 1453487 - KATIA CASTANHO SCORTECCI