Banca de QUALIFICAÇÃO: FABIANA MARIA COIMBRA DE CARVALHO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : FABIANA MARIA COIMBRA DE CARVALHO
DATA : 27/09/2019
HORA: 08:30
LOCAL: Sala A do Departamento de Nutrição (DNUT)
TÍTULO:

Evaluation of the effect of purified tamarind seed trypsin inhibitor on metabolic changes induced by experimental diet in a wistar rat model.

PALAVRAS-CHAVES:

Tamarindus indica L .. Lipid profile. Blood glucose. PPAR- γ. TNF-α.

PÁGINAS: 83
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

The increasing prevalence of obesity and all associated noncommunicable chronic diseases (NCDs) has increased the search for new methods to combat them. To investigate the effect of the nutritional pathways of nutritionally unbalanced diets on markers and/or parameters metabolic/biochemical, inflammatory and molecular, and to look for molecules that can act by reversing this situation is essential. In this study, an experimental diet was produced and its centesimal composition, glycemic index and glycemic load were analyzed. For glycemic index and glycemic load analyzes two groups of Wistar rats (n = 5) were used. The effect of this diet and tamarind seed purified trypsin inhibitor (ITTp) on metabolic changes induced by the experimental diet in Wistar rats was also evaluated. The experiments were conducted in two phases: 1) 17 weeks, in which two groups of Wistar rats (n = 5) were used, one group of animals were fed with the experimental diet, while the other group with the standard diet (Labina®). At the end of this phase, the animals were analyzed: nutritional status, markers and parameters biochemical, inflammatory, hormonal and molecular. 2)four groups of Wistar rats (n = 5) were used. Of these, three groups of animals were initially fed for 17 weeks with an experimental diet and presented obesity and then two of these groups were treated for 10 days a) standard diet b) ITTp (730 µg / kg). The third group of obese Wistar rats received no treatment (negative control) and one more group of eutrophic Wistar rats were added to the experiment as a positive control. On the 11th day of the experiment were evaluated: zoomometric measurements; consumption and weight gain; markers and parameters biochemical and molecular. The experimental diet showed high glycemic index (77.6) and high glycemic load (38.8). There was a statistically significant difference (p <0.0001) in the cumulative weekly difference in Lee Index values, evidencing an increase in the 17 weeks of study in the group that received the experimental diet. The animals that received the experimental diet presented increased and statistically different fasting glucose (p = 0.008) than the animals that received the standard diet. There were also statistically significant difference in relation to VLDL-c (p = 0.016) and TG (p = 0.016), being higher in the group of animals that received the experimental diet. Rats from the experimental diet group had significantly higher PPARγ mRNA relative expression in the gonadal and retroperitoneal tissues (p = 0.0121 and p = 0.0024, respectively). Related to the effect of pTTI on food intake (g), the pTTI-treated group had the lowest medians, significantly different from the experimental diet group (p = 0.0065). For the effect of ITTp on biochemical variables, the group of obese Wistar rats treated with ITTp had the lowest TG and VLDL-c concentrations (p = 0.0108) compared to the other groups. In addition, the group of animals treated with ITTp had significantly lower TNF-α mRNA relative expression (p = 0.025) compared to the other groups, regardless of PPAR-γ mRNA relative expression. The present study presents the ITTp as a bioactive molecule that acts on the modulation of metabolic changes caused by an high glycemic index experimental diet. Therefore, ITTp is of great biotechnological interest, especially for the development of biopharmaceuticals with potential application with anti-TNF-α function.

MEMBROS DA BANCA:
Externa ao Programa - 1879430 - ANA PAULA TRUSSARDI FAYH
Presidente - 1149356 - ELIZEU ANTUNES DOS SANTOS
Externa à Instituição - MAIRA CONCEIÇÃO JERONIMO DE SOUZA LIMA - UnP