Banca de DEFESA: JOHNY WYSLLAS DE FREITAS OLIVEIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOHNY WYSLLAS DE FREITAS OLIVEIRA
DATA : 30/08/2019
HORA: 09:00
LOCAL: Sala do POP - Centro de Convivência
TÍTULO:

CHARACTERIZATION OF ANTIPARASITY ACTIVITY OF DIETHYLDITICARBAMATE AGAINST DIFFERENT STRAINS OF Trypanosoma cruzi

PALAVRAS-CHAVES:

Chagas disease, Trypanosoma cruzi, DETC, Antiparasitic activity, DTU's.

PÁGINAS: 134
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Chagas disease prow by the protozoan Trypanosoma cruzi affecting millions of people. The drugs were later that cytotoxic are not recognized in the chronic phase of the disease. In addition, the parasite has a genetic variable that causes different clinical manifestations and resistance to treatments. Potassium diethyl dithiocarbamate belongs to the class of dithiocarbamates which are highly versatile chemical compounds that allow interaction with metals and cause the increase of oxidative free radicals. T. cruzi strains were characterized as cultivated in the environment. This is an epimastigote and trypomastigote assay of T. and is evaluated by the resistance reduction technique and clear camera counting. It analyzed the mechanisms of parasitic death triggered by the compound against T. cruzi epimastigote within 24 hours. Mitochondrial damage due to DETC under T. cruzi epimastigote after 24 hours of treatment by membrane potential assay. They were also analyzed as morphological changes by scanning electron microscopy of the DETC action after 24 hours of treatment. Cellular cytotoxicity of DETC was also analyzed for 3T3 and RAW plants 24 hours after compound application and MTT assay analyzes. Researchers examined the inhibitory action of DETC on proteolytic proteins of T. cruzi extracts through zymography and analyzed by densitometry. To have that which dithiocarbamate has a high case in the development of T. cruzi carbonic one sought to evidence the anchorage of the DETC. From the antiparasitic assays was the first action antiparasitic activity under the strains variations and the concentration required to eliminate the amount of 0.235 µm to 0.790 µM up to 0.664 µM for epimastigote and 0.235 µM up to 0.790 For trypomastigote according to strain . DETC cellular cytotoxicity analysis showed moderate cytotoxicity against animal species. When analyzing the mechanisms of cell death, it was noted the need to determine the cell death processes by the disease anchorage, ie, evidencing the non-expression of phosphatidylserine. Through mitochondrial damage testing, it is possible to perform a strong movement of DETC in different strains inhibiting mitochondrial membrane potential, reaching 80% reduction. Through the analysis of scanning electron microscopy, we observed the discontinuity in the parasite's cell membranes, as well as the rupture and formation of pores. The zymography showed a reduction in the potential of proteolytic activity of parasite extracts that reached 36%. Finally, which is DETC, it has a high interaction capacity to inhibit carbonic α-anhydrase by analyzing the positioning of its anchorage in the protein structure. Therefore, the DETC is an excellent alternative for the alternative treatment of Chagas disease due to antiparasitic activity, not presenting high cytotoxicity and triggering actions on the specific parasite compartments. In addition, an α-Anhydrase was identified. as possible the DETC.

MEMBROS DA BANCA:
Externa à Instituição - ALINE RIMOLDI RIBEIRO - Bordeaux
Externo ao Programa - 1715109 - DANIEL DE LIMA PONTES
Interno - 2195251 - HUGO ALEXANDRE DE OLIVEIRA ROCHA
Presidente - 2275890 - MARCELO DE SOUSA DA SILVA