Banca de QUALIFICAÇÃO: AMANDA JUSSARA FIGUEIREDO RÊGO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : AMANDA JUSSARA FIGUEIREDO RÊGO
DATA : 27/05/2019
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich - Departamento de Bioquímica
TÍTULO:

DNA methylation and dietary profile of non-syndromic oral cleft children

PALAVRAS-CHAVES:

cleft lip and palate, dietetic profile, epigenetic, DNA methylation

PÁGINAS: 97
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Non-syndromic cleft lip and/or palate (NSCL/P) is a birth defect with environmental and genetic causes. Epigenetic factors, such as DNA methylation, have been thought to influence heritability of this complex malformation and nutrition is strongly associated with the methylation pattern variability throughout life. Therefore, this work aimed to analyze the DNA methylation pattern of global genome and BRCA1 promoter in children with oral clefts. In addition, it was investigated the dietary profile of this patients to discuss the association between these factors and the risk of developing chronic diseases. The study was conducted with NSCL/P children attending at the Pediatric Hospital Professor Heriberto Ferreira Bezerra, in Natal/RN, and their mothers; it also included a control group composed by apparently healthy children without clefts and their mothers. Exfoliated buccal cell from all children were collected to perform DNA extraction. To determine global genome methylation, it was performed combined bisulfite restriction analysis of 5-methylcytosine (5-mC) from LINE-1 regions, and methylation specific polymerase chain reaction for BRCA1 promoter. Besides, an interview were carried out to obtain nutritional, socio-demographic and others information from the participants. The median percentage of LINE-1 5-mC for control and case groups were, respectively, 48% and 45.3%. The difference between this methylation levels were statistically significant (p-value < 0.05). The BRCA1 promoter was methylated in 40.9% and 59.1% of control and case groups, respectively and in 60% and 40% of the same groups, it was both methylated and unmethylated. Regarding the dietary status, it was observed that NSCL/P group had a high consumption of simple carbohydrates (e.g. refined wheat flour, white rice and sugar) and ultra-processed foods (such as snack, artificial seasoning and candy) together with a low intake of plant based and raw foods (like nuts, grain, vegetables and tubers). This profile signals to a health imbalance and low support for epigenetic regulation required for physiological homeostasis and health maintenance.The results indicate that NSCL/P children have a global hypomethylation pattern in comparison with children not affected and a poor food intake quality. Thereby, it is necessary to improve lifelong health guidelines for these children in order to prevent other chronic metabolic diseases and the inheritance of this phenotype to other generations.

MEMBROS DA BANCA:
Presidente - 1199127 - SILVIA REGINA BATISTUZZO DE MEDEIROS
Interna - 2261797 - TIRZAH BRAZ PETTA LAJUS
Externa ao Programa - 2323511 - ADRIANA AUGUSTO DE REZENDE