Banca de DEFESA: CAMILA RAYANE PEREIRA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAMILA RAYANE PEREIRA DA SILVA
DATA : 07/06/2019
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich - Departamento de Bioquímica
TÍTULO:

IN SILICO CHARACTERIZATION AND ANALYSIS OF EXPRESSION OF A HOMOLOGOUS SEQUENCE TO SEC14 IN SUGARCANE

PALAVRAS-CHAVES:

SECRETION14;Saccharum spp; RT-qPCR; Lipid binding domain; abiotic stress.

PÁGINAS: 103
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Brazil is the world's largest producer of sugarcane. All parts of this cultivar are used as energy matrix for production not only of sugar, but also of biofuels and other products. Molecular studies with sugarcane develop slowly due to their genomic complexity; however, the discovery of new genes that may interfere in key processes of this organism is valuable for future contributions in the sugar- alcohol industry. This work aimed to characterize a sequence homologous to PHOSPHATIDYLINOSITOL-PHOSPHATIDYLCHOLINE TRANSFER PROTEIN SEC14 in sugarcane that has been identified in previous studies in subtractive libraries for the flowering process. The in silico characterization was performed by functional analysis of the gene, construction of protein-protein interaction networks, phylogeny, molecular dynamics of the three-dimensional model of the protein and co-expression analysis, in addition, an RT-qPCR expression assay was performed in plant tissues for late and early varieties, as well as in leaf tissues submitted to drought and oxidative stress, as well as expression analysis in different tissues of the plant. The results obtained using the bioinformatics tools show the existence of the CRAL-TRIO domain that is present in all homologous sequences essentially in the same position, revealing high conservation of the sequence SEC14 in plants. Interaction networks reveal that the protein homologous to SEC14 in A. thaliana can interact with the proteins of the PATTELIN family (PTL), SYNTAXIN (SYP), OXYSTEROL BINDING PROTEIN (ORP) , PHOSPHOLIPASE D (PLD), among others, whose main functions include transport of the lipid phosphatidylinositol, vesicle traffic, and can act on the action of phytormones such as abscisic acid, stress responses, development of floral organs and membrane integrity. The phylogenetic tree suggests that there are likely duplications of the SEC14 protein in plants. The three-dimensional model presents a probable hydrophobic pocket for lipid binding, described in other consolidated SEC14 models, indicating its structural conservation. Expression data reveal that the SEC14 gene was differentially expressed in the early juvenile phase variety and in the late mature variety; in addition, there was no significant difference in expression in leaf tissues of plants submitted to drought and oxidative stress, probably due to the juvenile stage of the analyzed organism. Finally, this study provides information for a better understanding of how the gene / protein of SEC14 acts on sugarcane. They reveal new knowledge about the three-dimensional structure of this protein in this culture; also brings new perspectives for molecular studies with plants of economic interest such as sugarcane.

MEMBROS DA BANCA:
Externa à Instituição - DAIANE CRISTINA FERREIRA GOLBERT - UFRN
Presidente - 1453487 - KATIA CASTANHO SCORTECCI
Externo à Instituição - TERCILIO CALSA JUNIOR - UFPE