Banca de QUALIFICAÇÃO: MATHEUS ANSELMO MEDEIROS

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : MATHEUS ANSELMO MEDEIROS
DATA : 25/02/2019
HORA: 10:00
LOCAL: Sala Carl Peter von Dietrich - Departamento de Bioquímica
TÍTULO:

Effects of creatine supplementation in redox state in diabetic rats induced by streptozotocin

PALAVRAS-CHAVES:

creatine, diabetes, kidney

PÁGINAS: 45
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Introduction: Creatine is a supplement that has been the subject of research in several pathologies, with a wide possibility of clinical applications. Studies have shown the effect of creatine on increasing glucose uptake by elevating the expression of GLUT-4 receptors in metabolic disorders such as Type 2 Diabetes Mellitus, however, there are no studies that report the effect of creatine on the glucose metabolism of type Diabetes Mellitus 1. Thus, this work aims to evaluate the effect of creatine supplementation in diabetic rats induced by streptozotocin. Methods: Thirty-two Wistar rats were divided into 4 groups: (C) animals without diabetes and without creatine supplementation (n = 8), (CCr) animals without diabetes and with creatine supplementation (n = 8), (D) animals streptozotocin-induced diabetes mellitus (n = 8), (DCr) creatine-treated streptozotocin-induced diabetes mellitus (n = 8). Groups D and DCr will receive a single dose of streptozotocin (40 mg/kg i.p.). The following evaluations were performed: clinical, biochemical, as well as the analysis of redox state parameters in the renal tissue of the animals. Results: The groups D and DCr showed higher water and feed intake compared to groups C and CCr. In the weight variation in the experimental period, the diabetic groups showed significant weight loss in relation to the control groups. In the relative renal weight, the diabetic groups presented significant hypertrophy; in addition, the DCr group presented significantly greater renal hypertrophy than the D group. In fasting glycemia, the diabetic groups showed significant hyperglycemia in comparison with the control groups, however, the DCr group showed a significant improvement in relation to the group D. There was no statistical difference between the groups in serum creatinine and aspartate transaminase levels. In the quantification of urea and alanine transaminase, the diabetic groups showed a significant increase in comparison with the control groups, however, the DCr group showed a significant improvement in relation to the group D in both parameters. The enzymatic activity of catalase, superoxide dismutase and glutathione peroxidase were significantly lower in group D compared to control groups, however, the DCr group had restored activity like the control groups in both enzymes. In the quantification of the hydrogen peroxide content, the D group had significantly higher levels in comparison to the control groups, however, the DCr group had a level like the control groups in this parameter. We observed in the quantification of thiobarbituric acid reactive substances in the CCr group a significant increase in relation to the other experimental groups. In the quantification of carbonylated proteins, group D showed levels significantly lower in relation to the other groups, while the DCr group showed values statistically like the control groups. In the quantification of soluble thiols, there was no difference between the experimental groups. Conclusion: Creatine can improve metabolic profile in DM and restore redox state parameters in this condition. These results support creatine supplementation as a potential therapeutic agent.

MEMBROS DA BANCA:
Externo ao Programa - 1667882 - BENTO JOAO DA GRACA AZEVEDO ABREU
Interno - 1267860 - GUSTAVO ANTONIO DE SOUZA
Presidente - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN