Banca de QUALIFICAÇÃO: CAMILA RAYANE PEREIRA DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAMILA RAYANE PEREIRA DA SILVA
DATA : 18/12/2018
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich - Departamento de Bioquímica
TÍTULO:

CHARACTERIZATION OF A HOMOLOGOUS SEQUENCE TO SEC 14 OF SUGAR CANA

PALAVRAS-CHAVES:

SECRETION14; Saccharum spp; RT-qPCR; CRAL-TRIO; abiotic stress.

PÁGINAS: 100
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Brazil is the world's largest producer of sugarcane. All parts of this cultivar are used as energy matrix for production not only of sugar, but also for biofuels and other products. Molecular studies with sugarcane develop slowly due to their genomic complexity; however, the discovery of new genes that may interfere in key processes of this organism is valuable for future contributions in the sugar-alcohol industry and advances in the molecular studies of this cultivar. This work aimed to characterize a sequence homologous to PHOSPHATIDYLINOSITOL-PHOSPHATIDYLCHOLINE TRANSFER PROTEIN (SEC14) in sugarcane that has been identified in previous studies in subtractive libraries for the flowering process. The characterization in this work was made by in silico analysis and assay of expression in plant tissues for late and early variety by RT-qPCR, besides analysis of the expression in leaf tissues submitted to hydric and oxidative stress. The results obtained using the bioinformatics tools show the existence of the CRAL-TRIO domain that is present in all homologous sequences essentially in the same position, revealing high conservation of the sequence SEC14 in plants. Interaction networks reveal that the protein homologous to SEC14 in A. thaliana can interact with the proteins of the family PATTELIN (PTL), SYNTAXIN (SYP), OXYSTEROL BINDING PROTEIN (ORP), PHOSPHOLIPASE D (PLD), among others, whose functions lipid transport phosphatidylinositol, vesicle traffic, and may act on the action of phytormones, such as abcisic acid, stress responses, development of floral organs and membrane integrity. The phylogenetic tree suggests that there are probable duplications of the SEC14 protein in plants. The three-dimensional model presents the probable hydrophobic pocket for lipid binding described in other consolidated SEC14 models, indicating its structural conservation. Expression data revealed that the SEC14  gene differentially expressed in the early juvenile phase variety and in the late mature variety, in addition, there was no significant difference in expression in leaf tissues of plants submitted to hydric and oxidative stress. The data corroborate for a better characterization of SEC14 in sugarcane and an understanding of how this gene / protein acts in this cultivar, bringing new perspectives to molecular studies with plants of economic interest such as sugarcane.

MEMBROS DA BANCA:
Externo à Instituição - CARLOS HENRIQUE SALVINO GADELHA MENESES - UEPB
Interno - 1228866 - EDUARDO LUIZ VOIGT
Presidente - 1507794 - RODRIGO JULIANI SIQUEIRA DALMOLIN