Banca de DEFESA: ANDERSON FELIPE JÁCOME DE FRANÇA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ANDERSON FELIPE JÁCOME DE FRANÇA
DATA : 31/08/2018
HORA: 08:00
LOCAL: Sala SS1 - Departamento de Biologia Celular e Genética
TÍTULO:

GLOBULIN PROTEIN OF SEEDS OF Lachesodendron viridiforum: AN ALTERNATIVE FOR COMBATING MICROBIAL AGENTS AND TUMOR CELLS

PALAVRAS-CHAVES:

Lachesiodendron viridiflorum, proteins, multifunctional, promiscuous.

PÁGINAS: 73
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Plant seeds are reservoirs of molecules with great potential for elaboration of bioproducts and for this reason a special attention has been directed in the search of bioactive proteins with antimetabolic action and pharmacological properties. Some seeds present proteins and peptides that alone play multiple roles, such as interactions with membranes of bacteria causing disruption, fungicidal and antitumor activity, in the recruitment of macrophages and neutrophils an action defined as immunomodulation and hemolytic activity. A prospection of proteins with multifunctional activity was carried out in four final strata of the Fabaceae family: Senna spectabilis (Cassia-do-northeast), Anadenanthera colubrina (Angico), Adenanthera pavonina (Carolina) and Lachesiodendron viridiflorum (Jurema-Juquiri). A protein was found in L. viridiflorum, called LvP. When analyzed by SDS-PAGE, it had a molecular mass of approximately 17 kDa, with high hemolytic activity and cytotoxic activity against mononuclear cells. It did not present toxic activity to 3T3 cell line. However, when confronted with the HeLa, Hep G2, HT29, B16, A-375 and A2058 tumor lines, a high inhibition rate of cell viability was observed at low contractions. The same was observed for fungi Candida albicans, C. tropicalis, C. dubliniensis, C. glabrata and C. parapilosis. However, when tested against pathogenic bacteria Staphylococcus aureus, Escherichia coli and Methicillin-resistant Staphylococcus aureus - MRSA, no bactericidal activity was detected at the concentrations tested. When LvP was associated with the reference antibiotics, and tested again against bacteria, it was able to reduce the antibiotic MIC up to one dilution for all three species, thus characterizing a synergistic activity of LvP. Based on the data obtained, the isolated LvP corresponds to a multifunctional protein structure, and its anti-tumor, antimicrobial, fungicide and bactericidal properties, need to be better investigated so that its biotechnological potential is explored.

MEMBROS DA BANCA:
Interno - 1549705 - ADRIANA FERREIRA UCHOA
Externo à Instituição - ANA KATARINA MENEZES DA CRUZ SOARES - UnP
Presidente - 1149356 - ELIZEU ANTUNES DOS SANTOS
Externo à Instituição - JAILMA ALMEIDA DE LIMA - CRI
Externo à Instituição - RANIERE FAGUNDES DE MELO SILVEIRA - F.M.Nassau