Banca de DEFESA: JÉSSICA MARINA DE PAIVA PEREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JÉSSICA MARINA DE PAIVA PEREIRA
DATA : 29/06/2018
HORA: 14:00
LOCAL: Anfiteatro das Aves
TÍTULO:

Development of molecular tools to identify genotypic variants of the virus that causes White Spot Syndrome in shrimps

PALAVRAS-CHAVES:

WSSV, ORF94, ORF75, variable-number tandem repeats, Genotyping, Whispovirus

PÁGINAS: 94
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

The northeast region is responsible for more than 90% of the shrimp production in Brazil, and the state of Rio Grande do Norte is the second largest producer in the country. White spot syndrome virus (WSSV) has been considered one of the main challenges faced by global shrimp farming in the last decades, but its epidemiological status has been little studied in Brazil to date. The genetic variability of WSSV has been investigated mainly for conducting studies concerning viral dispersion and in attempts to correlate genotype and virulence. This variability is accessed primarily through the variable regions ORF23 / 24 and ORF14 / 15, and tandem repetitive regions occurring within ORF75, ORF94 and ORF125. The primers used to genotype WSSV based on these regions have been described for at least 10 years, and some do not work efficiently to identify new WSSV variants including those occurring in Brazil. In this work, new sets of PCR primers were developed based on alignments of all sequences available so far. The new primers were synthesized and new PCR reactions were standardized and had their efficiency compared with protocols and primers already published. The new primers were used in a pilot epidemiological study in which shrimp samples were collected from farms located along the coast of Rio Grande do Norte. The prevalence of WSSV in the state was verified and the samples with the highest viral load were genotyped. The new set of primers proved to be more efficient, allowing a larger number of samples to be genotyped. The results indicate that WSSV prevalence in the state of Rio Grande do Norte was high at the time the samples were collected. The genetic variability of WSSV in the state is low and was observed mainly in the ORF94 marker. The results allowed to observe some evidence that events of genetic recombination may have originated the Brazilian isolate of WSSV.

MEMBROS DA BANCA:
Presidente - 1880243 - DANIEL CARLOS FERREIRA LANZA
Interno - 1674709 - VIVIANE SOUZA DO AMARAL
Externo à Instituição - PATRICIA RAQUEL NOGUEIRA VIEIRA - SEAP