Banca de QUALIFICAÇÃO: MARIA LÚCIA DA SILVA CORDEIRO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : MARIA LÚCIA DA SILVA CORDEIRO
DATA : 25/04/2018
HORA: 14:00
LOCAL: Sala Carl von Dietrich
TÍTULO:

PHYTOCHEMICAL CHARACTERIZATION FROM LEAVES EXTRACTS FROM OF Commiphora leptophloeos (Mart.) J.B. Gillett. (BURSERACEAE)  AND EVALUATION OF ANTIOXIDANT EFFECT AND CYTOTOXIC

PALAVRAS-CHAVES:

Prospection, Imburana de espinho, extract, Bioactive compounds, Caenorhabditis elegans

PÁGINAS: 88
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Biologia Molecular
RESUMO:

Commiphora leptophloeos is a plant Caatinga biome. In this work, it was obtained extracts from fresh leaves. It was done the phytochemical characterization and the antioxidant potential in vitro and in vivo. It was also analyzed the cytotoxicity effect on 3T3 (normal cell) and B16 tumor lineage cells.Through a serial extraction, with solvents in growing order of polarity extracts were obtained: hexanico (EH), chloroformic (CE) ethanolic (EE), methanolic (EM), residual aqueous (EAR). Besides, it was done another extract called as water extract (EA) similar as it was used in folk medicine. The phytochemical characterization was done by measuring the phenolic compounds, total proteins and sugars. It was also done a qualitative tests, analysis by thin-layer chromatography (CCD) and ultra-high performance liquid chromatography (UHPLC). It was also analyzed the in vitro antioxidant activity and cell viability assay by MTT assays. The results from the qualitative phytochemical screening allowed the identify different groups from secondary metabolites. The TLC showed the presence of phenolic compounds, terpenes, flavonoids, phenolic acids, tannins and saponins in special at the EE and EM. The Co-TLC analysis showed the presence of flavonoids luteolin and isoquercetina at EE and EM. UHPLC analysis identified rutin in these samples. The antioxidant assays showed that all extracts have the antioxidant capacity in vitro, in special the EE and EM. Based on these results, it was analyzed the effect from EE on the animal model Caenorhabditis elegans. These in vivo assays showed that the EE was able to reduce in 50% of the intracellular levels of ROS from this animal model. Viability assays showed that these extracts were not cytotoxic on 3T3 cells (normal) and they were able to reduce the viability from B16 tumor cells on 50%. Taken together, these results indicate that C. leptophloeos has a potential source of bioactive compounds with antioxidant and antitumor potential. 

MEMBROS DA BANCA:
Externo ao Programa - 2492944 - CAROLINE ADDISON CARVALHO XAVIER DE MEDEIROS
Externo à Instituição - DIEGO DE ARAUJO SABRY - IFRN
Presidente - 1871916 - RAQUEL BRANDT GIORDANI