Banca de QUALIFICAÇÃO: JULIANA FERNANDES DOS SANTOS DAMETTO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JULIANA FERNANDES DOS SANTOS DAMETTO
DATA : 28/03/2018
HORA: 08:30
LOCAL: Sala de Reuniões do Centro de Biociências
TÍTULO:

EVALUATION OF MATERNAL SUPPLEMENTATION WITH VITAMIN AND ON THE CONCENTRATION OF RETINOL IN SERUM AND MATERNAL MILK

PALAVRAS-CHAVES:

Vitamin A. Alpha-tocopherol. Dietary supplementation. Puerperium. Human milk. Vitamin A Deficiency.

PÁGINAS: 143
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

One of the major public health problems in the world is vitamin A deficiency, which may occur due to insufficient dietary intake or poor physiological utilization. Vitamin E deficiency, while causing silent symptoms, is also characterized by a serious health problem and can lead to complications for the development of newborns and children. Supplementation of postpartum women may be a strategy to combat vitamin A and E deficiency. Animal studies have shown that vitamin E has a positive synergism with vitamin A bioavailability. The main objective was to show the effect of maternal supplementation with alpha-tocopherol on serum retinol concentration and human milk up to 60 days postpartum. This study was prospective, controlled, randomized, starting with 80 women attended for delivery in two public maternity hospitals in Rio Grande do Norte. In the immediate postpartum period, these women were allocated to the control groups (n = 18) without any intervention; supplemented 1 (n = 16) receiving the dose of 400 IU RRR-alpha-tocopherol; and supplemented 2 (n = 19) receiving the dose of 800 IU of RRR-alpha-tocopherol and being followed up to 60 days postnatal. Maternal blood was collected in four moments: 1th (0 hour), 20th and 30th and 60th days, as well as the collection of maternal milk in the following moments: 1th (0 hours), 2th (24 hours), 7th, 20th, 30th and 60th days. On the 7th, 20th, 30th and 60th days, dietary information was also collected. Retinol and alpha-tocopherol were analyzed by High Performance Liquid Chromatography. The intake of vitamin A from the puerperae and the supply of the vitamin to the infant through breast milk were evaluated according to consumption recommendations for the age group. The concentration of maternal serum retinol at the 0 hour time point for the control group was 43.8  14 μg / dL, for the supplemented group 1 it was 42.5 12.6 μg / dL and for the supplemented group 2 it was 34, 9 7.5 μg / dL, not differing between groups (p> 0.05), being indicative of adequate nutritional status. After supplementation, the percentage of retinol increased in the supplemented group 2 until the 30th day after delivery. Regarding vitamin E, at 0 hour the serum alpha-tocopherol concentration for the control group was 1212.6  341.3 μg / dL, for the supplemented group 1 it was 1201.5 420 μg / dL and for the group group 2 was 1020.7 260.3 μg / dL, with no significant difference between the groups (p> 0.05), with a satisfactory nutritional status as a diagnosis. After supplementation, the percentage of alpha-tocopherol increased in the supplemented group 2 until the 20th day postpartum. It was possible to suggest an intervention strategy to improve the status of both vitamins, combating the deficiencies, as it was found that supplementation with the dose of 800 IU RRR-alpha-tocopherol, administered in the immediate postpartum, ensured higher circulating levels of alpha-tocopherol up to 20 days postpartum and retinol up to 30 days postpartum. When the effect of alpha-tocopherol supplementation on the concentration of retinol in breast milk was observed, the three groups evaluated had a similar concentration of retinol in the milk (p> 0.05). The impact of supplementation on breast milk in the immediate postpartum period can be observed both in the group that received the dose of 400 IU RRR-alpha-tocopherol and the one that received the 800 IU, because it caused a percentage increase in the concentration of retinol 24 hours after the supplementation of ~ 74.1% and ~ 68.7%, respectively. Evaluating the supply in relation to the daily requirement of vitamin A, supplementary group 1 contemplated the requirement established for infants up to 6 months of age (400 g / day) only in milk production up to 24 hours postpartum. Supplementary group 2, however, supplied the daily vitamin A requirement of the infant until the 20th day after delivery. Given this scenario, it is possible to evidence the synergy of vitamin E over vitamin A, which may be a favorable factor in combating its deficiency. Regarding the analysis of alpha-tocopherol in breast milk, its concentration in the 0 hour milk was similar in all groups evaluated (p> 0.05). The percentage increase of alpha-tocopherol provided 24 hours after supplementation was high in both supplemented groups, but group 2 presented a higher percentage, remaining increased until the 7th day of the research. Thus, it was concluded that vitamin E supplementation administered in the immediate postpartum period offered improvements in the maternal nutritional status in relation to vitamin A and also its milk supply during the course of this study, and this increase in retinol is greater dose of vitamin E administered. This situation occurred in a group where maternal nutritional status was diagnosed as adequate for both vitamin A and vitamin E, and thus, alpha-tocopherol can act as a helper in the biochemical process of retinal transport in both serum and milk maternal health. This situation should be reassessed in conditions of nutritional deficiency.

MEMBROS DA BANCA:
Presidente - 2578619 - ANA HELONEIDA DE ARAUJO MORAIS
Externo ao Programa - 1837366 - DANIELLE SOARES BEZERRA
Externo ao Programa - 1674112 - RENATA ALEXANDRA MOREIRA DAS NEVES