Banca de QUALIFICAÇÃO: ANDREW DOUGLAS MOURA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ANDREW DOUGLAS MOURA
DATA : 28/02/2018
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich - Departamento de Bioquímica
TÍTULO:

MAPPING OF B AND T-CELLS EPITOPES ON THE SEQUENCES OF AMA-1, GRA-7 AND SAG-1 PROTEINS from t. GONDII AND CONSTRUCTION OF CHIMERIC antigens WITH POTENTIAL USE FOR DIAGNOSIS AND VACCINE AGAINST TOXOPLASMOSIS.

PALAVRAS-CHAVES:

Toxoplasmosis, Immunoinformatic, epitopes, vaccines, diagnosis

PÁGINAS: 110
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Toxoplasmosis is a worldwide zoonosis caused by the protozoan Toxoplasma gondii (T. gondii), which can infect several warm-blooded animals, including humans. The most severe symptoms are usually observed in immunocompromised patients, could be even fatal. Due to the great biological diversity of T. gondii non-clonal isolates circulating in the southern hemisphere of the terrestrial globe, severe complications of the disease can also be observed in immunocompetent individuals, such as myocarditis, encephalitis, and ocular complications. Recently, a study revealed that T. gondii is also associated in the delineation of neurodegenerative diseases, such as Parkinson's and Alzheimer's, and some cancers. Advances in immunoinformatics has been collaborating with strategies to improve the search and identification of immunodominant epitopes contained in proteins of microorganisms, which can be recognized by host B and T cells. The aim of this study was to map, analyze and select B and T epitopes contained in the Toxoplasma gondii AMA1, GRA7 and SAG1 proteins, as well as to construct chimeric peptides formed by the junction of B and T epitopes, analyzed their immunogenic potential in silico and in vitro.Four chimeric peptides were constructed, which showed binding affinities with murine and human MHC molecules in through in silico analyzes. A multi-antigenic chimera (TgAGS / BsT) composed of all the epitopes of these different proteins, was constructed, analyzed in silico, and later obtained through the expression in recombinant system. Our preliminary in vitro results, through antigenic stimuli in PBMC culture of immunocompetent IgG negative and Toxoplasmosis positive patients, induced the expression of CD25 + in CD3 + in the group of positive patients with significance when compared to the negative patients. These findings open perspectives for the development of vaccines and diagnostic reagents for toxoplasmosis through chimeric products formed by B and T epitopes.

MEMBROS DA BANCA:
Externo ao Programa - 1046091 - JOAO FIRMINO RODRIGUES NETO
Interno - 1046922 - LEONARDO CAPISTRANO FERREIRA
Presidente - 2261797 - TIRZAH BRAZ PETTA LAJUS