Banca de DEFESA: ANA KARINA DE LIMA NASCIMENTO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ANA KARINA DE LIMA NASCIMENTO
DATA : 21/12/2017
HORA: 14:00
LOCAL: sala Carl Von Dietrich
TÍTULO:

IMMUNOMODULATING ACTIVITY OF DIFFERENT EXTRACTS OBTAINED from THE SPECIES Plukenetia volubilis (EUPHORBIACEAE)

PALAVRAS-CHAVES:

citocine, NO, RAW cells, in vivo assays, qRT-PCR, amendoim inca

PÁGINAS: 126
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
SUBÁREA: Química de Macromoléculas
RESUMO:

Inflammation is a response from the organism to aggression and injuries. This process may be finely regulated to prevent the emergence of diseases. Due to the importance of this field, the research of potential plants with immunomodulators activities has receiving an important role.  Considering this aspect, in this work it was analyzed the effect of different plant extracts from Plukenetia volubilis L. (Euphorbiaceae stricto sensu ss) for the immunomodulatory activity. In order to do this, it was used in vitro (RAW 264.7 macrophages) and in vivo (BALB / C mice) models. The extracts used were: methanolic (ME), aqueous (AE), hexane (HE), ethanolic (EE) and chloroform (CE). The results obtained showed that the extracts from P. volubilis stimulated the activity of MTT reduction by the mitochondrial dehydrogenases of the macrophages and that there was no cytotoxicity at the concentrations used (100, 250 and 500 μg / mL). Macrophages were stimulated with LPS and then, it was treated with plant extracts. It was observed a significant reduction in the production of nitric oxide (NO) when it was compared to the control assay. The percentages of NO production for ME, AE, HE, EE and CE extracts were 56%, 64%, 64.1, 65% and 72%, respectively. Furthermore, it was measured the cytokine and the relative expression by qPCR. The data obtained showed in generally a reduction from the cytokines and its expression (qPCR). Only for TNF-α qPCR was observed a relative expression above the control. The extracts: HE, CE and AE probably may be act by the inhibition from COX-2 and iNOS by NfΚB transcription factor pathway. On the other hand, for TNF-α its action mechanism may be related to an independent pathway. The in vivo assay showed that EE and AE inhibited the migration of leukocytes to the peritoneal cavity from mice induced to peritonitis. Thus, these results in vitro and in vivo allowed us to conclude that the extracts from P. volubilis may have potential biomolecules that may be used for the treatment of inflammatory diseases in future as these extracts might be act as modulating the inflammatory response through cytokines.

MEMBROS DA BANCA:
Externo à Instituição - DAIANE CRISTINA FERREIRA GOLBERT - UFRN
Externo à Instituição - JAILMA ALMEIDA DE LIMA - CRI
Presidente - 1453487 - KATIA CASTANHO SCORTECCI
Externo à Instituição - RANIERE FAGUNDES DE MELO SILVEIRA - UFRN
Interno - 1871916 - RAQUEL BRANDT GIORDANI