Banca de DEFESA: LARISSA QUEIROZ DE LIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : LARISSA QUEIROZ DE LIRA
DATA : 08/12/2017
HORA: 13:30
LOCAL: Sala Carl Peter von Dietrich - Departamento de Bioquímica
TÍTULO:

Effect of two maternal supplementation protocols with alpha-tocopherol on serum and milk of breastfeeding women up 60 days after delivery.

PALAVRAS-CHAVES:

Alpha-tocopherol. Clinical trial. Nutritional Supplementation. Lactation. Breastfeeding. Vitamin E deficiency.

PÁGINAS: 125
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Vitamin E deficiency (VED) is associated with serious complications to the health and development of newborns and children, generating subsequent effects in their adult life. Among the strategies to combat this deficiency, maternal supplementation during lactation presents itself as a viable conduct, even though there is no definition of an ideal protocol, mainly due to a lack of human studies. Thus, for the first time in humans, this study had as main objective analyzing the effect of two maternal supplementation protocols with alpha-tocopherol on the maternal biochemical nutritional status up to 60 days postpartum. For this purpose, eighty healthy lactating women were recruited at two public maternity hospitals in Natal – RN between 2013 and 2016. Eligible participants were randomly assigned to control, supplemented 1 or supplemented 2 groups, in a 1:1:1 ratio. Dietary information was collected on the 7th, 20th, 30th and 60th days postpartum. Serum and milk samples were collected at the 1st (0h), 20th, 30th and 60th days, with an additional milk collection on the 7th day. Immediately after the collection at day 1, the supplemented groups 1 and 2 received a (01) dose of 400 IU of RRR-alpha-tocopherol, and after the collection at day 20, the supplemented group 2 received the second supplemental dose. No supplementation was given to the control group. Alpha-tocopherol was quantified by high performance liquid chromatography. The usual vitamin E intake and its supply through milk were assessed according to specific recommendations (16 mg and 4 mg daily, respectively). Based on the results, none of the participants had adequate vitamin E intake throughout lactation. This characteristic was similar between the groups (~ 5.0 mg/day, p = 0.603), thus assuring the non-influence of maternal diet on the results of the supplementation schemes. For the three groups, serum alpha-tocopherol concentrations corresponding to the four postpartum periods were indicative of adequate nutritional status, with no differences between them regarding serum 0h (p > 0.05). Values in milk 0h were also not different between the groups (p > 0.05). As lactation progressed, due to the physiological decline of alpha-tocopherol in serum (p < 0.01) and breast milk (p < 0.01) in the three groups, it was observed an increase in both the percentage of maternal VED and inadequate supply through milk. However, such increases were less intense in the supplemented group 2 as result of the double alpha-tocopherol dose. Compared to the control group, the double supplementation protocol enabled an increase of 36% in serum alpha-tocopherol and 160% in milk at day 30, reaching the 60th day with values 27% greater in serum and 59% in milk, and an 84% adequacy in the vitamin E supply to the infant (3.2 mg/day). On the other hand, the single-dose supplementation showed no effect on maternal serum during lactation and promoted an increase in milk vitamin E only up to the 20th day (35%), not being able to maintain the adequacy of vitamin supply to the infant at the end of the study (2.5 mg/day). Hence, since the maternal supplementation protocol with two doses of 400 IU of RRR-alpha-tocopherol showed a greater and longer lasting effect on vitamin E in serum and breast milk over the sixty days postpartum, it can thus be established as a starting point for the definition of a viable and safe protocol that optimizes and maintains the maternal nutritional status, as well as the quality of milk composition throughout lactation.

MEMBROS DA BANCA:
Externo ao Programa - 1837366 - DANIELLE SOARES BEZERRA
Externo à Instituição - HERYKA MYRNA MAIA RAMALHO - UNP
Externo à Instituição - ILLANA LOUISE PEREIRA DE MELO - UnP
Presidente - 180.956.404-25 - ROBERTO DIMENSTEIN - UFRJ
Interno - 1149522 - SUELY FERREIRA CHAVANTE