Banca de QUALIFICAÇÃO: GUSTAVO HENRIQUE OLIVEIRA CAVALCANTE

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : GUSTAVO HENRIQUE OLIVEIRA CAVALCANTE
DATA : 22/11/2017
HORA: 14:30
LOCAL: Sala de Reuniões do Centro de Biociências
TÍTULO:

Study of structural elements in human papillomavirus genome and development of molecular tools to aid diagnosis.

PALAVRAS-CHAVES:

Human papillomavirus, cervical cancer, PCR, genotyping, selective pressure, diversity

PÁGINAS: 93
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Human papillomavirus (HPV) is a small DNA virus characterized as one of the most common sexually transmitted agents worldwide, whose accurate detection and genotyping is only possible by using molecular biology techniques. Among the more than 180 different genotypes already characterized, different evolutionary dynamics and biological properties have been reported, so that a particular group of HPVs is strongly related to chronic infections and progression to cancers such as cervical, anal, vulvar, vaginal, oropharyngeal and penile. A new set of primers was developed for HPV detection and genotyping using the polymerase chain reaction (PCR) technique, targeting a conserved region of variable size within the E1 viral gene. This system allowed the discrimination of the major oncogenic genotypes by analyzing the size of the amplified fragments by agarose/polyacrylamide gel electrophoresis, with confirmation by direct sequencing, reducing the time and cost required for the diagnosis. From the data obtained in molecular analyzes during the development of the proposed method, analyzes of genomic variability and selective pressure were also performed, through phylogenetic reconstructions by different methods, estimation of variability parameters and application of regression models for the identification of sites under positive selection throughout the coding regions. Understanding the viral diversity and evolution is an important tool for the future development of new diagnostic and therapeutic approaches to this global public health issue.

MEMBROS DA BANCA:
Interno - 1513597 - JOAO PAULO MATOS SANTOS LIMA
Externo ao Programa - 348473 - JOSE VERISSIMO FERNANDES
Presidente - 1046922 - LEONARDO CAPISTRANO FERREIRA