Banca de DEFESA: TATIANA KUMMER DA ROCHA PINHEIRO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : TATIANA KUMMER DA ROCHA PINHEIRO
DATA : 05/10/2017
HORA: 16:00
LOCAL: Sala Carl Peter von Dietrich
TÍTULO:

 

Genetic factors of susceptibility to leprosy in Rio Grande do Norte, Brazil

PALAVRAS-CHAVES:

 

Leprosy reactions. M. leprae. Erythema Nodosum Leprosum. Reversal reaction. Immunochip. SNP.

PÁGINAS: 69
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

 

Leprosy is a chronic, slowly evolving infectious disease with a broad spectrum of clinical manifestations caused by Mycobacterium leprae. The state of Rio Grande do Norte has a low detection coefficient for new cases, but some localities have focal areas with high detection rates, such as the city of Mossoró, which had a detection rate of 39.73 per 100,000 habitants in 2012. After exposure to the bacillus, about 10% of people develop disease, with a spectrum of presentations, ranging from the tuberculoid pole (tuberculoid-tuberculoid, borderline-tuberculoid), the borderline (borderline-borderline), to the lepromatous pole (lepromatous-lepromatous). The World Health Organization also classifies the disease according to the number of lesions, for therapeutic purposes, so cases with up to five lesions are classified as paucibacillary (BP), and cases with more than five lesions, multibacillary (MB). Approximately one third of people with leprosy develop immunopathological reactions, classified as type I, type II and neuritis. The post-infection evolution with M. leprae is influenced by environmental factors and by the genetic repertoire of the host. Therefore, in order to contribute to the knowledge of the association of genetic factors with susceptibility to leprosy, the objective of the present study was to analyze single nucleotide polymorphisms (SNPs) associated with susceptibility to leprosy in the newborn. The study design was casecontrol with recruitment of cases of leprosy and contacts. The participants were phenotypically characterized according to exposure to M. leprae, considering the presence of anti-LID-NDO antibody and clinical presentation. The amount of antibodies varied according to the operational classification of the leprosy case and the type of reaction, being higher in MB cases and type II reaction. All participants were also genotyped using Immunochip. For analysis of the genotyping data were considered: leprosy vs contacts, reaction vs non-reaction, and anti-LIDNDO antibody rate. A total of 55 SNPs showed association with leprosy and antibody levels. In the leprosy vs contacts group, 10 SNPs showed association, 3 related to the immune response (FASLG, TNFS18, EBF1 and ICOSLG), as well as one SNP close to VDR, whose protein is related to immunomodulation associated with vitamin D3 in monocytes, macrophages and activated lymphocytes. In the reaction vs. non-reaction group, the association of 30 SNPs, 20 close to genes known as susceptibility to psoriasis, and one SNP close to the UBD gene were observed. In the continuous LID-NDO antibody rate results, the association with 15 SNPs, one related to the THEMIS gene, which encodes a regulatory protein in T-cell selection was seen. Among the 55 SNPs associated with one of the phenotypes, 4 are found in coding regions. The results indicate that Immunochip is a tool to identify genes susceptible to leprosy development and its reactions. Together the data suggest that susceptibility to leprosy and the development of leprosy reactions are linked to the cellular and humoral immune response of the host and could potentially be modulated.

MEMBROS DA BANCA:
Externo ao Programa - 1714243 - DANIELLA REGINA ARANTES MARTINS SALHA
Externo à Instituição - LUCAS PEDREIRA DE CARVALHO - UFBA
Presidente - 350647 - SELMA MARIA BEZERRA JERONIMO