Banca de QUALIFICAÇÃO: ADRIANA MARINA E SILVA PARENTE

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : ADRIANA MARINA E SILVA PARENTE
DATA : 23/08/2017
HORA: 09:00
LOCAL: Sala SS1 - Departamento de Biologia Celular e Genética
TÍTULO:

STRUCTURAL EVALUATION AND ANALYSIS OF THE BIOLOGICAL ACTIVITIES OF STIGMURIN

ANALOGUE PEPTIDES IN THE Tityus stigmurus VENOM

PALAVRAS-CHAVES:

Antimicrobial peptide; Analog peptides; Antiproliferative activity; Bioactive peptides; Scorpion; Tityus

stigmurus.

PÁGINAS: 46
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Tityus stigmurus it’s the predominant scorpion specie in Northeast region from Brazil, it’s considered one of the main cause of scorpion accidents. In T. stigmurus venom, which is a complex mixture of molecules of high and low molecular mass, was identified an antimicrobial peptide called Stigmurin. Antimicrobial peptides are small molecules that are considered the first line of defense against microorganisms, they show broad spectrum action. Many authors have proved that these peptides can also actuate on cancerous cells. Mutation in the sequence of these molecules has been held aiming the activity increase with low toxicity. Therefore, it is proposed the characterization of the structure in silico and by circular dichroism, as well the antimicrobial, anti-proliferative and hemolytic activity of two analog peptides from Stigmurin was evaluated. We performed an in silico analysis for these peptides, we were able to observe α-helix structure, which was confirmed by circular dichroism. We were also able to find that the analog peptides net charge and hydrophobic moment were higher than Sitgmurin’s, which which can explain the increase in antimicrobial activity. The peptides StigA3 e StigA4 showed activity on cancerous cells similitar to the native peptide, except when tested on a normal cell we were able to find that they’re less toxic. Therefore, these results indicate a potential biotechnological application for the analogs peptides, even as prototype to new therapeutic agents.

MEMBROS DA BANCA:
Externo à Instituição - ARIANE FERREIRA LACERDA - UFRN
Externo ao Programa - 3652554 - FRANCISCO CANINDE DE SOUSA JUNIOR
Presidente - 1513597 - JOAO PAULO MATOS SANTOS LIMA