Banca de QUALIFICAÇÃO: NATÁLIA CABRAL SOUZA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : NATÁLIA CABRAL SOUZA
DATA : 05/06/2017
HORA: 13:00
LOCAL: Sala SS1 - DBG/CB
TÍTULO:

Anti-inflammatory and antioxidant properties of Turnera subulata and Anarcadium occidentale

PALAVRAS-CHAVES:

Oxidative stress. Inflammation. Lipopolysaccharide (LPS)

PÁGINAS: 67
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Extract of plants are widely used in traditional medicine, mainly in the northeastern region of Brazil. The use of extract the leaves of Turnera subulata and Anacardium Occidentale, for example, is made as an alternative in the treatment of various diseases such as diabetes, hypertension, chronic pain, cancer and inflammation in general. Despite their wide use, the effects of these compounds are not yet well described in the literature. Thus, we sought to evaluate the antioxidant and anti-inflammatory properties of leaf extracts of T. subulata and A. occidentale in a model of inflammation in vitro, using as a stimulus the LPS under the macrophage cells of strain RAW 264.7. Evaluating the ability to induce and/or inhibit phosphorylation in MAPK (p38, ERK ½ and JNK). In addition to the quantification of inflammatory response markers in the lineage. From this we observed that the extracts presented antioxidant and anti-inflammatory activities. Therefore, we observed that the co-treatment with leaf extract of T. subulata was able to reduce the oxidative stress in the cells, generated by the inflammatory response. One of the main points was that it was observed that this extract was able to directly modulate the inflammatory response, altering the activity of the members of the MAPK pathways. Our results also show that extract of A. occidentale exhibits antioxidate activity. The antioxidant effect was observed when the extract decreased the oxidative damage in cells of macrophages treated with the dosages of 0.5 μg / mL, 5 μg / mL. In addition, this extract reversed the oxidative damage and inflammatory parameters induced by lipopolysaccharide (LPS) in the cells tested. The extract at the dosages of 0.5 μg / mL, and 5 μg / mL was also able to inhibit the release of TNF-α and IL-1β in LPS-induced cells. Inflammatory markers such as TLR4, RAGE and CD40 that are induced by LPS were also modulated. Later, we evaluated the signaling pathways involved in the LPS-mediated inflammatory response. The extract of A. occidentale blocked the effects of LPS on the phosphorylation of ERKÎ ±, SAPK / JNK and P38. Taken together, our results indicate the antioxidant and anti-inflammatory effects of the above aqueous extracts and reveal possible biological mechanisms involved in these effects.

MEMBROS DA BANCA:
Interno - 1228866 - EDUARDO LUIZ VOIGT
Externo à Instituição - JAILMA ALMEIDA DE LIMA - CRI
Presidente - 1453487 - KATIA CASTANHO SCORTECCI