Banca de QUALIFICAÇÃO: ANA CAROLINA PEREIRA ROCHA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANA CAROLINA PEREIRA ROCHA
DATA : 06/06/2017
HORA: 08:00
LOCAL: Sala Carl Peter von Dietrich
TÍTULO:

Transcriptome analyzis of human mesenchymal stem cell during osteogenesis

PALAVRAS-CHAVES:

RNAseq, stem cell, Osteoblast, ncRNA

PÁGINAS: 7
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

The differentiation of human mesenchymal stem cells (hMSC) into osteoblast follows a specific program of gene expression, committing itself initially under the influence of Wnt pathways and the BPMs that direct the cell to its transformation into osteoblast. However, the specific activation pathways during the early phase of differentiation are still poorly explored.Thus, the objective of the present work was to characterize the transcriptional profile of the beginning of the osteogenesis from hMSC obtained from the vein of the umbilical cord, of data obtained through the sequencing of the new generation Illumina RNA-Seq. Data from the complete transcriptome were analyzed in Transcriptogramer for the production of an ordered list of functionally associated genes, obtained by the mean of the gene expression taken on neighboring genes in this list, thus facilitating their biological interpretation. To study gene ontology and gene expression profile design during osteoblastogenesis, the metabolic processes and molecular functions significantly altered during the course of the differentiation process were analyzed in several tools (REVIGO, GOrila, PANTHER, LNCipedia and NONCODE) and properly described. During hMSC differentiation, an increase in expression of osteoblastic phenotype genes was observed as soon as the first day of differentiation started. Noncoding RNAs were also identified, depending on the evolution of the differentiation process, as well as genes involved in the formation of membrane rafts, on the third day of differentiation. During the third day of induction, cell differentiation regulation genes and other important genes on biological processes which precede differentiation, such as cell adhesion, signaling and external chemical factors response, have already increased expression. The study of gene expression during these first three days also revealed the decrease in the expression of groups of genes crucial to basal metabolism maintenance, RNA degradation and cytoskeleton organization.

MEMBROS DA BANCA:
Externo à Instituição - DANIEL CHAVES DE LIMA - IFRN
Externo ao Programa - 348.475.768-02 - JOSÉ EDUARDO KROLL - USP
Presidente - 1251018 - RIVA DE PAULA OLIVEIRA