Banca de QUALIFICAÇÃO: LUCIANA MARIA ARAUJO RABELO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : LUCIANA MARIA ARAUJO RABELO
DATA : 17/10/2016
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich - Depto. de Bioquímica - CB/UFRN
TÍTULO:

Cytotoxic Effects of a Serine Protease Inhibitor Purified from Juquiri (Mimosa regnellii Benth) Seeds on Melanoma B16-F10 Cells

PALAVRAS-CHAVES:

Mimosa regnellii; Trypsin inhibitor; B16-f10; Cell death; Leguminosae.

PÁGINAS: 137
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

Cancer is a term used to represent a set of more than 100 diseases, including malignant tumors of different localizations. Among the main existing types, cutaneous melanoma stands: is a type of skin cancer that originates in melanocytes and is the most common in Brazil, accounting for 30% of all malignant tumors registered in the country. In most cases, melanoma at an early stage can be treated with surgery alone, but in more advanced cancers it is required other treatments. Sometimes, more than one type of treatment is used. In this study, a purified Kunitz trypsin inhibitor from Leguminosae Mimosa regnellii Benth (ITJ) seeds was tested for its ability to induce cell death by apoptosis pathway in mouse melanoma cells B16-F10 and to evaluate its apoptotic capacity; the inhibitor was also evaluated for its ability to influence the release of reactive oxygen species, calcium release, cell morphologic changes and inhibition of angiogenic activity of rabbit endothelial cells (ClPs). Tests for toxicity against normal cells were also performed, confirming that ITJ acts specifically on melanoma cells with an IC50 of 0,65 uM. These results suggest that ITJ has potential for use as drug in the treatment for melanomas.

MEMBROS DA BANCA:
Externo à Instituição - DIEGO DE ARAUJO SABRY - IFRN
Externo ao Programa - 2524706 - GIULIANNA PAIVA VIANA DE ANDRADE SOUZA
Externo à Instituição - LUDOVICO MIGLIOLO - UCB