Banca de QUALIFICAÇÃO: MILENA SIMÕES PEIXOTO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : MILENA SIMÕES PEIXOTO
DATA : 05/08/2016
HORA: 09:00
LOCAL: Sala de reuniões do CB
TÍTULO:

Oxidative stress and cell death by particulate matter from the Amazon burning and isolated compounds

PALAVRAS-CHAVES:

biomass burning, retene, oxidative stress, mitochondrial integrity, cell death

PÁGINAS: 81
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

In recent discussions on environmental issues, air pollution has been growing as a concerning topic, as it  may lead to serious complications for both the environment and public health. Several poluents are released daily by natural or human activities, causing the air to be improper and harmful to the welfare of humans and ecosystems. In the Amazon region, deforestation and forest fires have been causing damage to the exposed population. Therefore, the aim of this study was to evaluate oxidative stress, mitochondrial integrity and cell death of organic chemical compounds from particulate matter smaller  than 10 μm (PM₁₀) originated from biomass burning of the Amazon forest, as well as the effects of retene, a biomass burning marker, in lung epithelial cells (A549). Regarding the extracted organic particulate matter, this led to the increased production of reactive oxygen species and intracellular mitochondrial superoxide. Additionally, PM₁₀ exposure triggered the formation of autophagosomes, suggesting increased autophagy. In the biological analysis with retene, the data showed that this compound led to an increase in reactive oxygen species and mitochondrial superoxide, hyperpolarization of the mitochondrial membrane, as well as increased mitochondrial content at all times. However, the retene was only able to induce cell death in the greatest concentration used and over a 72-hour period. From these results, it is important to emphasize the reduction of emissions by biomass burning, searching for new control policies. In addition, the toxicity of the retene raises an alert about the inclusion of this compound in the risk assessment of PAHs, mainly because it is the most abundant component in biomass burning.

MEMBROS DA BANCA:
Externo ao Programa - 2087759 - ANDRE DUCATI LUCHESSI
Presidente - 2085604 - SUSANA MARGARIDA GOMES MOREIRA