Banca de DEFESA: YAGO QUEIROZ DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : YAGO QUEIROZ DOS SANTOS
DATA : 16/06/2016
HORA: 14:00
LOCAL: Sala Carl Peter von Dietrich - Depto. de Bioquímica - CB/UFRN
TÍTULO:

Production, purification and characterization of a salt-tolerant thermostable cellulase from a marine Bacillus sp. strain 

PALAVRAS-CHAVES:

Sugarcane bagasse-degrading bacteria, Thermostable enzymes, Bioprocessing

PÁGINAS: 13
GRANDE ÁREA: Ciências Biológicas
ÁREA: Bioquímica
RESUMO:

The current demand for environment-friendly renewable energy sources has driven the search for alternatives capable of replacing the use of fossil fuels. One of the most promising innovations to positively impact the world energy scenario is the second-generation bioethanol production (B2G) from reducing sugars derived from enzymatic degradation of lignocellulosic material that is ordinarily discarded at agroindustrial processes. In this work, a salt-tolerant cellulase  secreted by a  marine Bacillus sp. SR22 strain  with wide resistance to  temperature and pH was isolated and characterized. The 37.35 kDa endoglucanase named as Bc22Cel was purified by ammonium sulphate precipitation, gel filtration chromatography and extraction from the gel after nonreducing sodium dodecylsufate-polyacrylamide gel electrophoresis. The optimal pH value and temperature of Bc22Cel were 6.5 and 60 °C, respectively. The purified Bc22Cel showed a considerable halophilic property being able to maintain more than 70% of residual activity even when pre-incubated with 1.5 M NaCl for 1 hour. Kinetic analysis of purified enzyme showed the Km and Vmax to be 0,3953 nmol ml-1 and 0,0167 µmol ml-1 min-1, respectively. Taking together, the present data indicates the Bc22Cel as a potential and useful candidate for industrial applications.

MEMBROS DA BANCA:
Externo à Instituição - CICERA RAQUEL FERNANDES RODRIGUES - UNILEÃO
Presidente - 1149356 - ELIZEU ANTUNES DOS SANTOS
Externo ao Programa - 1452833 - MARIA CELESTE NUNES DE MELO
Interno - 1997012 - RAQUEL CORDEIRO THEODORO