Banca de DEFESA: MIGUEL HIGINO NETO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MIGUEL HIGINO NETO
DATE: 03/09/2021
TIME: 14:30
LOCAL: Google meet
TITLE:

Development and characterization of polycaprolactone/chitosan blends with N-methyl-D-glucamine by Solution Blow Spinning method for application in cutaneous leishmaniasis

KEY WORDS:

Blends scaffolding; Polycaprolactone (PCL); Chitosan; Solution Blow Spinning (SBS); Leishmaniasis.

PAGES: 68
BIG AREA: Engenharias
AREA: Engenharia Química
SUBÁREA: Tecnologia Química
SPECIALTY: Têxteis
SUMMARY:

The concern with a rapid healing of chronic wounds, such as those observed in skin lesions caused by Leishmania parasites, has become the reason for several study groups to develop high-performance dressings. This new approach to textile nanotechnology seeks to develop dressings that promote the controlled release of drugs directly to the wound site more quickly and effectively. This study aimed to develop nanofiber blends using the Solution Blow Spinning (SBS) technique, based upon the combination of the biopolymers Polycaprolactone (PCL) and Chitosan, with the incorporation of N-methyl-D-glucamine, the drug of choice for the treatment of cutaneous leishmaniasis. Initially, a solution was prepared containing the concentration of 25% (w/v) and 75% (w/v) of Chitosan and PCL, respectively, diluted in 99.8% glacial acetic acid. In a second solution, under the same conditions described above, the drug N-methyl-D-glucamine at a concentration of 6.7% (w/v) was included. Both solutions were homogenized by stirring a magnetic bar for 24 hours at room temperature. After obtaining, the solutions were transferred to a syringe attached to the ejection pump to obtain the nanofiber. Scanning electron microscopy (SEM) and infrared spectroscopy (FTIR), were used to characterize the nanofibers. The release of NMDG from this new drug delivery system was analyzed using visible light spectroscopy (UV-VIS). The production process developed in this research allowed to obtain blending scaffolding by combining the biopolymers PCL and Chitosan, without and with the drug N-methyl-D-glucamine, using the solution blowing technique (SBS). From the SEM analysis, it was possible to observe significant differences in the mean diameter of the scaffolding blends, which showed values of 320.9 ± 93.5 nm in the nanofiber without drug and 365.8 ± 113.2 nm for the nanofiber with the drug (p <0.001). The FTIR characterization indicates in the fibers the presence of functional groups characteristic of each material used and produced, allowing to detect the incorporation of the drug with an increase of 6.2% in the transmittance of the nanofibers. The mixing of two polymers proved to be an effective technique to obtain a new material with desirable properties, which may be relevant to optimize drug incorporation the drug delivery, and targeting properties. The study also proved the effectiveness of the SBS technique for obtaining nanofibers with chitosan. Although in vitro biological activity studies are necessary to prove the biological application of nanofibers, our data point to the promising use of PCL / chitosan nanofibers incorporated with NMDG for the treatment of cutaneous leishmaniasis.

BANKING MEMBERS:
Presidente - 1481705 - SALETE MARTINS ALVES
Interno - 2239386 - JOSE IVAN DE MEDEIROS
Externo à Instituição - FABIO ANDRE BRAYNER DOS SANTOS - Fiocruz - PE