Banca de DEFESA: ALINE GABRIEL FREIRE

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ALINE GABRIEL FREIRE
DATA : 27/02/2020
HORA: 14:00
LOCAL: A DEFINIR
TÍTULO:

NANOENCAPSULATION OF BIOACTIVE HEXAPEPTIDE WITH TEXTILE BIOPOLYMER FOR DERMOCOSMETICS APPLICATION

PALAVRAS-CHAVES:

Nanobiotechnology. Silk Fibroin. Nanocapsules. Acetyl Hexapeptide, Nanocosmetics. Textile Fibers

PÁGINAS: 85
GRANDE ÁREA: Engenharias
ÁREA: Engenharia Química
SUBÁREA: Tecnologia Química
ESPECIALIDADE: Têxteis
RESUMO:

The use of biopolymers derived from textile fibers in nanostructured complexes to deliver, administration and controlled release of drugs has emerged in several areas of technology. In the cosmetic area, the use of nanocapsules as encapsulating agent of active molecules has the advantage of protection against oxidation, photolysis and hydrolysis, better skin permeation, increased shelf life, controlled release, reduction of skin irritation, improvement of bioavailability, targeting from the drug to the dermis, among others. The extracted silk fibroin in fiber is a promising biopolymer for application in the biomedical area due to its characteristics of biocompatilidade, biodegradability, non-toxicity and microbial resistance. Acetyl hexapeptide-8 or acetyl hexapeptide-3, commercially known as Argireline ® is a synthetic macromolecule developed to mimic the botulinum toxin in the reduction of wrinkles and expression lines. The hexapeptide has a high molecular weight and has hydrophilic characteristic, which hinders the permeation to the deeper layers of the skin, crowding together only on the surface of the stratum corneum. The aim of this study was to develop a nanovetor to the molecule of acetyl hexapeptide using silk fibroin as an encapsulant, for potential implementation in dermocosmetics topical formulations that allow the controlled release of in a deep layer of the skin. The standard techniques have been prepared by the method of dessolvatation with nanoprecipitation in different concentrations. Nanoparticles have been analyzed as the morphology, size, efficiency of encapsulation, active release and cytotoxicity using dynamic light scattering techniques (DLS), zeta potential, scanning electron microscopy with effect from field (SEM-FEG), absorption spectroscopy in Fourier transform infrared (FTIR), cell viability and controlled release in vitro. Nanoparticles obtained had diameters between 70 and 150 nm, with spherical morphology. The FTIR results demonstrated the presence of the drug in the nanosystem. Nanoparticles loaded showed no cytotoxicity at a concentration of 0.1 mg/ml 0.05 mg/ml, making feasible the implementation in epithelial tissue.

MEMBROS DA BANCA:
Presidente - 2941160 - JOSE HERIBERTO OLIVEIRA DO NASCIMENTO
Interna - 2212519 - JULIANA DE SOUZA NUNES
Interna - 2614285 - KESIA KARINA DE OLIVEIRA SOUTO SILVA
Externa à Instituição - MARIA APARECIDA MEDEIROS MACIEL - UnP
Externa ao Programa - 1754360 - WALDENICE DE ALENCAR MORAIS LIMA