Banca de QUALIFICAÇÃO: JULIANA RAYSSA BARROS FELIX

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JULIANA RAYSSA BARROS FELIX
DATE: 07/08/2026
TIME: 09:00
LOCAL: Google Meet
TITLE:

Refining Environmental Metabarcoding for Glomeromycota (AMF): Development of the ALMA Pipeline and Application in Assessing the Impact on AMF Communities under Land-Use Change in the Amazon Deforestation Arc

KEY WORDS:

eDNA, Beta diversity, AMF, Environmental factors, Glomalin.

PAGES: 95
BIG AREA: Ciências Biológicas
AREA: Biologia Geral
SUMMARY:

This dissertation presents an integrative approach to the study of arbuscular mycorrhizal fungi (AMF). Initially, we establish best practices for fungal metabarcoding studies, highlighting essential steps from experimental design, sampling, DNA extraction, and barcode selection to bioinformatics and statistical analyses, complemented by the introduction of the automated pipeline ALMA for taxonomic classification based on phylogenetic placement. In an applied context, this pipeline was utilized and validated in the second chapter, which investigates the impacts of converting pristine Amazon forest into agricultural cropland on AMF communities and glomalin-related soil protein (GRSP) dynamics within the Amazon Deforestation Arc (Mato Grosso, Brazil). Combining morphological analysis and environmental DNA (eDNA) metabarcoding, the study revealed a complete taxonomic reorganization (species turnover) rather than a simple loss of biodiversity; the morphological approach detected 75 morphospecies (39 in the forest and 57 in the cropland), while metabarcoding identified 61 OTUs (41 in forest sites and 40 in cropland). Agricultural management homogenized environmental conditions, increasing soil pH and nutrient availability, which triggered a functional transition in life-history strategies (guilds): ruderal strategies dominant in undisturbed forest gave way to stress-tolerant (Acaulosporaceae) and competitive (Gigasporales) taxa in the cropland. Furthermore, the cropland environment exhibited intense disturbance-driven sporulation and a functional coupling between the AMF community and glomalin accumulation, which contributes to the stabilization of soil organic carbon. Finally, this work contributed several new species records for the Brazilian Amazon, demonstrating that while intensive agriculture disrupts original forest ecological networks, it also activates specialized AMF responses that actively mitigate soil degradation.

COMMITTEE MEMBERS:
Externo ao Programa - 1755051 - BRUNO TOMIO GOTO - nullPresidente - 1513597 - JOAO PAULO MATOS SANTOS LIMA
Externa à Instituição - JULIANA LUIZA ROCHA DE LIMA