Banca de QUALIFICAÇÃO: JOANNY RAYANE DA SILVA COUTINHO

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JOANNY RAYANE DA SILVA COUTINHO
DATE: 06/01/2023
TIME: 08:30
LOCAL: virtual
TITLE:

Evaluation of the antibacterial activity of ciprofloxacin and ceftriaxone
in isolates from the intestinal microbiota of cancer patients

KEY WORDS:

intestinal microbiota; cancer; resistance; chemotherapy treatment; opportunistic
bacteria.

PAGES: 78
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUMMARY:

Several studies show that the intestinal microbiota may change due to the damage
caused by chemotherapy treatment in the intestinal epithelium, facilitating the dissemination
of opportunistic multiresistant microorganisms resistant to antimicrobials during the
treatment of cancer patients. However, there is still little information in the literature about
this resistance generated by intestinal mucositis in patients undergoing chemotherapy.
Therefore, the objective of this study was to evaluate the minimum inhibitory concentration
(MIC) and the minimum bactericidal concentration (MBC) of ciprofloxacin and ceftriaxone
in cancer patients, in addition to associate their social characteristics and general aspects to
antibiotic use. For this, fresh stool samples were collected from 30 cancer patients. The

samples were seeded on Nutrient Agar (AN), Macconkey Agar (MC), and Salmonella-
Shigella Agar (SS) media, and the MIC and MBC of each patient were performed. Then a

questionnaire was applied on socioeconomic aspects, use of antibiotics and hygiene. The
collected data were analyzed using the chi-square test, analysis of variance (ANOVA), and
the Newman- Keusl test at a 5% significance level. Most patients declared themselves as

white (60%), female (93%), urban area residents (93%), had completed high school (30%),
and most made use of the antibiotic amoxicillin (50%) before searching. In the AN and MC
media, MIC results showed a resistance to the drug ciprofloxacin of 60% and 56.7%,
respectively; in SS medium, the MIC data showed a susceptibility of 73.3% to the drug
ciprofloxacin; regarding CBM, in the study most patients (80%) were bactericidal, killing
99.9% of exposed bacteria. The most frequent age group in the study was between 46 and 65
years (53.3%), and concerning the tumor location, 83.3% of the parasitized patients had
breast cancer. Regarding the stage of cancer, the majority (33.3%) were unable to inform,
but it was followed by a relevant number (30%) who were in stage III of the disease. There
was a higher symptomatological incidence of vomiting (88.7%) in patients in grade 0,
followed by diarrhea (66.7%) and nausea (50%). It is concluded that cancer patients
undergoing chemotherapy are more likely to trigger an imbalance in the intestinal microbiota
and increase the resistance of invasive microorganisms.

COMMITTEE MEMBERS:
Interna - 1049952 - DEYSIANE OLIVEIRA BRANDAO
Presidente - 1714262 - LILIAN GIOTTO ZAROS DE MEDEIROS
Interna - 1452833 - MARIA CELESTE NUNES DE MELO
Externo ao Programa - 1714290 - RENATO MOTTA NETO - null