Dissertation/Thèse

Clique aqui para acessar os arquivos diretamente da Biblioteca Digital de Teses e Dissertações da UFRN

2024
Thèses
1
  • EDUARDO RODRIGUES DE SENA
  • HEMATOLOGICAL EVALUATION AND INFECTIOUS DISEASES ASSOCIATED WITH MANIFESTATION OF GENERALIZED CANINE DEMODICOSIS IN ADULTS

  • Leader : MANUELA SALES LIMA NASCIMENTO
  • MEMBRES DE LA BANQUE :
  • MANUELA SALES LIMA NASCIMENTO
  • RENATA ANTONACI GAMA
  • VICENTE TOSCANO DE ARAÚJO NETO
  • Data: 28 févr. 2024


  • Afficher le Résumé
  • Canine demodicosis is an inflammatory disease of the skin of dogs caused by the proliferation
    excessive growth of the mite Demodex spp., normally a commensal of the skin. The clinical manifestation
    is related to host genetic factors, use of immunosuppressive drugs,
    neoplasms and decreased or failed immune response due to infectious diseases
    concomitants. The objective of the project was to carry out hematological, biochemical and
    infectious diseases associated with the manifestation of generalized canine mange caused
    by Demodex spp. in adult dogs. For this, 16 dogs with symptoms were recruited
    demodectic mange clinic, among which 7 affected the generalized form of the disease.
    The most prevalent skin lesion was alopecia (100% of dogs), followed by
    desquamation in 75% of the dogs evaluated. Pododemodicosis and erythema were present
    only in the 7 dogs diagnosed with generalized demodicosis. Serological tests
    revealed the presence of antibodies against Ehrlichia canis/ewingii in 100% of dogs
    evaluated. Furthermore, the presence of antibodies against Leishmania was detected
    infantum and Anaplasma platys/phagocytophilum in dogs carrying generalization of
    demodectic mange (28.6%), as well as Dirofilaria immitis (14.3%) antigens. All 7
    dogs with generalized demodectic mange showed monocytosis and MCHC values
    below the reference, among which 6 also had leukocytosis and/or
    thrombocytopenia. Furthermore, neutrophilia and eosinophilia were present in 4 dogs with the form
    generalized demodicosis, and lymphocytosis in only 1 of them. Biochemical assessment
    revealed elevated AST values in 3 dogs with generalized demodicosis and increased AST
    in just one of them. The urea and creatinine values were within the
    reference in all dogs with generalized demodectic mange. Taken together, the data
    demonstrate hematological and biochemical changes in dogs with demodicosis
    widespread. A better understanding of the factors that lead to development
    of generalized demodicosis in adult dogs may serve as a basis for the development
    of combined therapeutic strategies that optimize the resolution of the clinical condition, as well as
    how to impede its development.

2
  • MARIA EDUARDA PESSOA LOPES DANTAS
  • Spatial dynamics of SARS-CoV-2 Variants of Concern circulating in the state of Rio Grande Do Norte

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • GONZALO JOSE BELLO BENTANCOR
  • IGHOR LEONARDO ARANTES GOMES
  • JOAO PAULO MATOS SANTOS LIMA
  • JOSELIO MARIA GALVAO DE ARAUJO
  • Data: 29 févr. 2024


  • Afficher le Résumé
  • 4. Resumo em inglês (abstract): The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), initially detected in December 2019 in Wuhan, China, is the causative agent of a pandemic responsible for over 6.5 million deaths worldwide as of March 2022. Brazil accounts for approximately 700,000 of these deaths, with its highest lethality rates recorded during the spread of the Zeta, Gamma, Delta, and Omicron variants, identified by the World Health Organization in January 2021. In the state of Rio Grande do Norte, the circulation of Gamma and Delta variants, for example, coincided with increases in the number of cases and deaths. To understand the spatial dynamics of concern variants in Rio Grande do Norte, phylogenetic techniques with a maximum-likelihood statistical framework were applied to a dataset consisting of sequences between November 2020 and March 2022, representing a significant fraction (>68%) of the total sequences from Rio Grande do Norte (nTOTAL=1702) during this period. The epidemiological profile of the epidemic in the state demonstrated that the Gamma period coincided with the peak in the number of cases, deaths, and lethality (~4%), decreasing only when Delta was introduced. Phylogenetic analysis revealed a large number of external introductions, with 100% from other Brazilian states during the Zeta and Delta periods, 98% from other Brazilian states and 2% from other countries during the Gamma period, while in Omicron, there were 36% from other countries and 64% from Brazil. Regarding internal introductions among immediate regions, Natal stood out as the main origin in all lineages. Therefore, with the resumption of urban mobility and incipient vaccination, the P.2, P.1, AY.99.2, BA.1, and BA.1.1 lineages in Rio Grande do Norte established multiple simultaneous transmission networks, reaching all regions of the state, overloading the local healthcare system, and increasing the infection's lethality. In the face of the continuous circulation of the SARS-CoV-2 virus in the country and the possibility of new viral emergencies, understanding the local dynamics of viral spread is an essential tool in the development of efficient and scientifically-based public policies.

3
  • ELYELTON BESERRA DE CARVALHO
  • FREQUENCY OF INTESTINAL PARASITES IN PRESCHOOL CHILDREN FROM A KINDERGARTEN
    CENTER IN NATAL, RN.

  • Leader : LILIAN GIOTTO ZAROS DE MEDEIROS
  • MEMBRES DE LA BANQUE :
  • HENRIQUE ROCHA DE MEDEIROS
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • VANESSA SANTOS DE ARRUDA BARBOSA
  • Data: 29 févr. 2024


  • Afficher le Résumé
  • This study aimed to identify the frequency of intestinal parasites in preschool children
    at a kindergarten in Natal-RN and relate it to the socio-environmental factors involved. For this, 66 children were
    evaluated, who, with parental authorization, provided socioeconomic data, biological samples of feces, the subungual
    and perianal region. In addition, soil samples were also collected in the kindergarten. The samples were processed
    using the HPJ, Rugai, Willis, Graham and Ritche methods and the results were sent to those responsible. Linked to
    this, a health education action was carried out with kindergarten teachers to assess their prior knowledge about
    intestinal parasites and present the most common ones, especially those that affect children. The study population
    was made up of 53% females and 47% males, with ages ranging from one to six years. The majority (65%) were
    referred to as white, while 35% as black. Parasites were identified in 49% of biological samples, with 56% of
    individuals being monoparasitized and 44% being polyparasitized. The most common parasites were Ascaris 

    lumbricoides (23%), Entamoeba coli (17%) and Hookworms (15%). The presence of Hookworm eggs was identified in
    a single sample of subungual contents. No biological forms were identified in soil analyses. The frequency of parasites
    was related to socioeconomic and biological factors, with individuals living in the North Zone of the city and with low
    income being those with the highest frequency (58% and 55%, respectively). The kindergarten teachers knew, at
    most, three parasites, but did not understand their biological cycles, forms of transmission and prevention. At the end
    of the activities, they became aware of between six and seven parasites, especially those most common in children. It
    was concluded that health educational actions carried out with teachers can contribute to knowledge of preventive
    measures and control of intestinal parasites; that the frequency of parasites in preschoolers is a problem in Natal and
    that socially vulnerable individuals are more affected, especially those residing in the North zone, with low income
    and without basic sanitation. Control and prevention actions are necessary for this scenario to change.

4
  • LUCAS ALVES DE SOUSA GOMEZ
  • Role of Vitamin D and inflammatory markers in hospitalized patients in ICUs during different SARS-CoV-2 pandemic peaks
  • Leader : JANEUSA TRINDADE DE SOUTO
  • MEMBRES DE LA BANQUE :
  • CÁSSIO RICARDO DE MEDEIROS SOUZA
  • JANEUSA TRINDADE DE SOUTO
  • VALTER FERREIRA DE ANDRADE NETO
  • Data: 29 févr. 2024


  • Afficher le Résumé
  • The prognosis of covid-19 relies on several factors, including patient's immune
    response, metabolic and nutritional status and genetic characteristics of the virus. This
    study investigated the inflammatory profile, oxidative stress and vitamin D levels in
    patients admitted to intensive care units at Hospital do Coração (HC) and Hospital
    Giselda Trigueiro (HGT), infected with the B.1.1.33 strain (2020) and P.2 or P.1
    (Gamma) strains of SARS-CoV-2, respectively. Patients with positive RT-PCR were
    organized into two groups: the first group had their blood collected from April to May
    2020 (B.1.1.33). The second group had blood collected in January and February of

    2021 (P.2/P.1). We determined global and differential leukocyte counts, neutrophil-
    lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR), systemic levels of C-reactive

    protein (CRP), lactic dehydrogenase (LDH), creatinine, vitamin D, malondialdehyde
    (MDA), cytokines and chemokines. Based on hematological characteristics and blood
    marker levels, they were characterized by two different groups, observing that patients
    with P.2/P.1 (group 2 – G2) had a higher number of leukocytes, mainly neutrophils,
    with higher proportions of NLR and PLR when compared to patients B.1.1.33 (group 1
    – G1). Furthermore, higher levels of LDH, CXCL8 and MDA and lower levels of vitamin
    D, GM-CSF and IFN- were found in G2 patients. The neutrophilia observed together
    with elevated levels of CXCL8 and LDH in G2 patients suggests greater susceptibility
    to the occurrence of pyroptosis and NETosis, which may play an important role in the
    severity of lung damage and systemic compromise during Covid-19 caused by the P.2
    and P.1 lineages, in contrast to patients with a more ancestral lineage of the virus.

5
  • LUANDERSON CARDOSO PEREIRA
  • Insecticidal activity of fluralaner (Exzolt®) administered in chickens against triatomines

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • ANDRESSA NORONHA BARBOSA DA SILVA
  • PAULO MARCOS DA MATTA GUEDES
  • RITA DE CASSIA MOREIRA DE SOUSA
  • Data: 7 mars 2024


  • Afficher le Résumé
  • Triatoma infestans, Triatoma brasiliensis, Triatoma pseudomaculata and Rhodnius prolixus are vectors of Trypanosoma cruzi (T. cruzi), etiological agent of Chagas disease, and chickens are important blood food source to triatomines. The aim of this study was to evaluate the insecticidal activity of fluralaner (Exzoltâadministrated in chickens against triatomines (R. prolixus, T. infestans, T. brasiliensis and T. pseudomaculata). Twelve non-breed chickens (Gallus gallus domesticus) (n=12) were randomized according to weight into three groups, Group 1: negative control (n=4), Group 2: single dose 0.5 mg/kg fluralaner (Exzoltâ) (n=4), Group 3: two doses 0.5 mg/kg fluralaner (Exzoltâ) (n=4). To carry out the experiment, nymphs of 3rd, 4th and 5th instars of Rhodnius prolixus, Triatoma infestans, Triatoma brasiliensis and Triatoma pseudomaculata (n=10) were allowed to feed on chickens before treatment, and 1, 7, 14, 21, 28, 35 and 56 days after treatment and insect mortality determined. After the blood meal, feeding efficiency and mortality of triatomine nymphs were determined. The results demonstrate higher feeding success for R. prolixus, followed by T. infestans, T. brasiliensis and T. pseudomaculata, respectively. Treatment of chickens with fluralaner does not affect the blood feeding rate of triatomines. Treatment with two doses of fluralaner showed higher insecticidal efficacy for R. prolixus, T. infestans and T. brasiliensis, compared to treatment with single dose. Similar insecticidal efficacy was observed for T. pseudomaculata using treatment with one or two doses of fluralaner. Insecticidal activity of fluralaner (Exzoltâ) against triatomine bugs was observed up to 21 and 28 days after treatment with one and two doses of fluralaner, respectively. Together, our results demonstrate that chicken’s treatment with fluralaner (Exzoltâ) induces insecticidal activity against triatomines up to 28 days after treatment and could be used as a control measure for Chagas disease in endemic areas.

2023
Thèses
1
  • JOANNY RAYANE DA SILVA COUTINHO
  • Minimum inhibitory and bactericidal concentration of antimicrobials ciprofloxacin and ceftriaxone against bacterial isolates obtained from feces of cancer patients
  • Leader : LILIAN GIOTTO ZAROS DE MEDEIROS
  • MEMBRES DE LA BANQUE :
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • MARIA CELESTE NUNES DE MELO
  • RENATO MOTTA NETO
  • CAIO AUGUSTO MARTINS AIRES
  • Data: 27 févr. 2023


  • Afficher le Résumé
  • The intestinal microbiota may undergo changes due to the damage caused by chemotherapy treatment, facilitating the dissemination of opportunistic multiresistant microorganisms resistant to antimicrobials during the treatment of cancer patients. In this context, the aim of this study was to evaluate the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the antimicrobials ciprofloxacin and ceftriaxone in bacterial isolates obtained from the stools of 30 cancer patients treated at a Reference Center in the state of Rio Grande do Norte and 10 healthy individuals called the control group. For this, all research participants answered a questionnaire about socioeconomic conditions, eating and hygiene habits and use of antibiotics and the samples obtained were sown on Nutrient Agar (AN), Macconkey Agar (MC) and Salmonella-Shigella Agar media. (SS) and performed the MIC and MBC of the antimicrobials ciprofloxacin and ceftriaxone considering the concentrations of 0.5μg/mL to 32μg/mL. The collected data were analyzed using chi-square test, ANOVA and Newman-Keusl test. Most patients declared themselves white (60%) female (93%), urban area residents (93%), had completed high school (30%) and most (50%) used the antibiotic amoxicillin before beginning of the study. The most frequent age group observed was between 46 and 65 years (53.3%) and 83.3% of the patients had breast cancer. Regarding the stage of cancer, 30% had cancer in stage III of the disease and 33.3% were unable to inform, with a higher symptomatological incidence of vomiting (88.7%), followed by diarrhea (66.7%) and nausea ( 50%). Considering MIC, ciprofloxacin drug resistance of 60% in AN medium and 56.7% in MC medium, with 73.3% drug susceptibility in SS medium. Regarding ceftriaxone, bacterial samples showed sensitive MIC (100%) in all media. With regard to CBM, 80% of the isolated samples were bactericidal, killing 99.9% of the bacteria exposed to the drugs ciprofloxacin and ceftriaxone, at concentrations of 8μg/mL and 4μg/mL, respectively. In the control group, the MIC was sensitive (100%) , confirming the change in the MIC of fecal bacterial samples from cancer patients. It is concluded that cancer patients undergoing chemotherapy are more likely to trigger an imbalance in the intestinal microbiota and increase the resistance of invasive microorganisms.

2
  • LUCAS ABRANTES BATISTA
  • Natural infection by trypanosomatids in different species of animals in municipalities in the state of Rio Grande do Norte.

  • Leader : ANTONIA CLAUDIA JACOME DA CAMARA
  • MEMBRES DE LA BANQUE :
  • AMANDA SILVA DOS SANTOS ALIANÇA
  • ANTONIA CLAUDIA JACOME DA CAMARA
  • JOAO FIRMINO RODRIGUES NETO
  • Data: 28 févr. 2023


  • Afficher le Résumé
  • Trypanosomatids are uniflagellate protozoans of the Kinetoplastea subgroup that group the genera Trypanosoma and Leishmania, in which several pathogenic species have developed unique mechanisms to regulate their life cycles and adapt to a variety of hosts. The present study aimed to identify natural infection by Trypanosoma cruzi and Leishmania spp. in different species of animals in the state of Rio Grande do Norte, Brazil. Whole blood samples were collected from 206 animals from 3 mesoregions of the rural state and another four bone marrow and lymph node aspirates from dogs in the urban area were used in the study. The DNA extracted from the samples with phenol-chloroform was submitted to three PCR assays with different molecular markers: 18S (SSU) rRNA that detects Trypanosoma spp. and Leishmania spp., kDNA for detection of T. cruzi and HSP70 for detection of Leishmania spp. Trypanosomatid DNA amplified by 18S PCR was detected in 78.1% (164/210) of the samples, with the highest distribution of positive samples in different species of animals observed in the West mesoregion and the highest percentages of positivity observed in Equus caballus with 85.7% (12/14), Bos taurus with 83.3% (5/6) and Ovis aries with 82.0% (50/61). Amplification of the T. cruzi kDNA was observed in 47.1% (97/206) of the whole blood samples of the animals studied, with a greater distribution of positive samples being observed in different species of animals in the western mesoregion, in which Equus caballus and Canis familiaris were the animal species with the highest percentage of positive samples with 57.1% (8/14) and 52.4% (54/103), respectively. HSP70 PCR from Leishmania spp. amplified DNA in 100% of culture samples from urban dogs and was not observed in whole blood samples from rural areas. Positive samples in 18S PCRs and kDNA were compared, and positive samples were observed in all orders of animals, with emphasis on Canis familiaris (43/106), Equus caballus (6/14) and Bos taurus (3/6). The 18S amplified DNAs that resulted in excellent quality electropherograms were from four samples, all obtained from dogs. These sequences were compared with reference sequences, which revealed one DNA from T. cruzi and three from L. infantum. These results revealed a high number and diversity of infected domesticated animals, reinforcing the importance of better understanding the maintenance cycles of the parasites close to human dwellings.

3
  • PAULO ANTÔNIO DA ROCHA FERREIRA
  • Epidemiological Aspects of Rabies in Rio Grande do Norte (2005 – 2021)

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • JOSELIO MARIA GALVAO DE ARAUJO
  • JOSE VERISSIMO FERNANDES
  • JOAO FELIPE BEZERRA
  • Data: 10 mars 2023


  • Afficher le Résumé
  • Rabies is a serious and potentially fatal infectious disease that affects mammals,
    mainly dogs, but also other species of animals. It is caused by the rabies virus, a
    virus of the genus Lyssavirus and family Rhabdoviridae, transmitted through
    infected saliva, usually through a bite. Rabies is considered endemic worldwide, with
    a higher incidence in Africa, Asia and Latin America. The objective of this work is to
    describe epidemiological aspects of rabies in mammalian species in the state of Rio
    Grande do Norte (2005 - 2021). The records and notes book of the Animal Rabies
    Diagnosis Sector - LACEN/RN and the Laboratory Environment Manager - GAL
    were used as a source of information. In the period between october 2005 and
    december 2021, 5,649 samples were sent for rabies laboratory analysis at
    LACEN/RN, 5,632 samples from non-human mammals and 17 samples from
    humans suspected of rabies. Of the total number of samples from non-human
    mammals sent for diagnosis, 611 (10.8%) were positive for rabies. In the same
    period, a human sample was diagnosed positive for rabies (5.89%). Considering the
    studied species, samples of 2,507 bats were analyzed and 416 (16.6%) were
    positive for rabies. A total of 1647 specimens from domestic canines were examined

    and 17 (1.03%) were positive. Additionally, 688 samples of domestic felines were
    studied, and of these, four (0.59%) were positive. In the context of cattle, 148 were
    analyzed, and of these, 92 (62.20%) were positive. A total of 105 samples from foxes
    were studied and 59 (56.11%) were positive. As for the horses, 47 were studied and
    17 (36.18%) were positive. Of the 18 sheep analyzed, two (11.11%) were positive.
    Of the 17 human samples, one (5.89%) was positive. Of the 11 pigs analyzed, three
    (27, 28%) were positive for rabies. Of the three donkeys analyzed, 01 (33.33%) was
    positive. Of the 417 samples analyzed from non-human primates (NHP), 31
    opossums and 10 goats, none were positive for rabies. From this study, we can
    conclude that the rabies virus is circulating in mammals in the state of Rio Grande
    do Norte, Brazil, and because it is a neglected zoonosis with unique and complex
    epidemiological characteristics, it deserves more attention and study.

4
  • FÁBIO DO NASCIMENTO SALES
  • Antifungal potential of Lippia grata Schauer (Verbenaceae) essential oil against Candida and dermatophyte species”

  • Leader : VANIA SOUSA ANDRADE
  • MEMBRES DE LA BANQUE :
  • VANIA SOUSA ANDRADE
  • RAFAEL WESLEY BASTOS
  • EULÁLIA CAMELO PESSOA DE AZEVEDO XIMENES
  • Data: 24 mars 2023


  • Afficher le Résumé
  • Species of the genus Candida are responsible for about 80% of serious fungal infections, being Candida albicans the most commonly isolated specie in these cases, besides tobefrequently associated with cutaneous and mucocutaneous fungal infections. Amongthefilamentous fungi, the dermatophytes that cause cutaneous mycoses in humans affect 25%of the world population and 18% to 23% of the Brazilian population. In the last decades, Candida and dermatophyte species, as well as several other fungal species, have showedresistance development to conventional antifungal drugs used in clinical therapy. Thus, inface of the scenario of emerging drug-resistant fungal pathogens, it is perceivedanincessant search of the scientific community for natural compounds that present antifungal potential, such as those of plant origin, due to their many antimicrobial properties describedin the literature. Therefore, the present proposal aims to evaluate the antifungal activityof the essential oil from the plant Lippia grata Schauer (Verbenaceae) against yeasts of theCandida genus and different species of dermatophyte fungi. The essential oil was obtainedfrom the hydrodistillation process, while its phytochemistry was performed bygaschromatography associated with mass spectrometry. The bioactivity of the essential oil obtained from the Lippia grata plant was investigated on 30 fungal samples by the methodof Minimum Inhibitory Concentration (MIC) by the microdilution broth technique andMinimum Fungicidal Concentration (MFC) by the “spot” sowing method. To evaluatesynergistic activity, the “checkerboard” method was used. The quantification tests of biofilm production and antibiofilm activity of Lippia grata essential oil were performedusing crystal violet to measure the optical densities obtained. Four main phytoconstituentswere identified in the chemical characterization of Lippia grata essential oil: carvacrol (51.4%) as the majority constituent, thymol (15.6%), ρ-cymene (12.2%) and γ-terpinene(7.4%). Antifungal activity of Lippia grata essential oil was found for 100%of the testedstrains with MIC ranging from ≤ 0.00075 mg/mL to 3.12 mg/mL and MFC ranging from≤0.00075 mg/mL to 25 mg/mL against Candida strains while the MIC/MFC ranged from≤0.00075 mg/mL to 0.39 mg/mL for dermatophytes. Regarding the evaluation of thesynergistic effect between the essential oil of Lippia grata and the antifungal on the strainsthat presented high MICs of essential oil, it was observed that this combinationwasindifferent to 50% of the strains tested. The results of biofilm formation revealed that 100%of the strains were biofilm producers. For Candida strains, 4 (21.05%) were weakproducing strains, 8 (42.11%) moderate producing strains and 7 (36.84%) strong producingstrains. For dermatophyte strains, 1 (9%) weak producing strains, 2 (18%) moderateproducing strains and 8 (73%) were strong producing strains. For the antibiofilmactivityof the essential oil, it was verified inhibition of cell viability (IC50%) ranging from57.5%to72.4% for Candida strains and 42.4% to 96.4% for dermatophytes, when these strains werepost-treated, respectively, with concentrations of 0.78 mg/mL, 1.56 mg/mLand3.12mg/mL of Lippia grata essential oil. It was concluded that the essential oil of Lippia gratashowed antifungal activity in the assays, motivating new studies with the intention of futuredevelopment of phytodrugs directed to the treatment of fungal infections.

5
  • LÉA ISIS MARTINS GOMES
  • Evaluation of the antiviral effect of Zinc in SARS-CoV-2 infection model

  • Leader : PAULA RENATA LIMA MACHADO
  • MEMBRES DE LA BANQUE :
  • JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS
  • KLEBER JUVENAL SILVA FARIAS
  • PAULA RENATA LIMA MACHADO
  • Data: 31 mai 2023


  • Afficher le Résumé
  • Infection with SARS-CoV-2 can trigger an exacerbated inflammatory response, characterized by excessive activity of the immune system in an attempt to protect the organism from the pathogen. Upon recognition of viral particles, a very aggressive immune response is triggered, which may cause immunopathologies mediated by the release of pro-inflammatory cytokines, in addition to cytopathic events in infected cells. Zinc (Zn) is an essential micronutrient, which plays important physiological roles in cellular processes, from the immune response, signal transduction, organelle homeostasis to cell proliferation, and is already well known for its antioxidant, anti-inflammatory,  immunomodulatory and antiviral. Some studies relate the antiviral role of Zn to immunity, linking its effectiveness against COVID-19. The antiviral properties of Zn can occur from reducing viral infection, inhibition of viral protease and polymerase. Thus, the objective of this study was to evaluate the antiviral action of Zn and to analyze its therapeutic potential in cells of Calu-3 lineages infected by SARS-CoV-2, as well as to verify the role of oxidative stress in culture of pulmonary epithelial cells infected by Zn. SARS-CoV-2. For this, an in vitro assay was performed, in which Calu-3 cells were infected with SARS-CoV-2 and treated with different concentrations of Zinc and Cobalt, which was used as a codependent control, and cells and supernatant were collected for evaluation of viral load, cell viability, inflammatory parameters and oxidative stress. Among them, the cytotoxicity assay, LDH dosage, cytokine dosage, oxidative stress dosage through flow cytometry and viral load quantification by plaque assay (PFU). Our data demonstrate that Zn was able to inhibit viral replication in Calu-3, in addition to having an anti-inflammatory effect by decreasing IL-6 and TNF-a production, decreasing oxidative stress and cell death profile. These data suggest that Zn has potential antiviral effect as an adjuvant therapy and may provide protection by decreasing lung inflammation.

6
  • LARISSA HENRIQUE PONTES
  • EVALUATION OF ARTIFICIAL FEEDER FOR Lutzomyia longipalpis AND IMPROVEMENTS IN THE USE OF CDC TRAP BATTERIES.

  • Leader : RENATA ANTONACI GAMA
  • MEMBRES DE LA BANQUE :
  • RENATA ANTONACI GAMA
  • ANDREY JOSÉ DE ANDRADE
  • ROSELI LA CORTE DOS SANTOS
  • Data: 27 juil. 2023


  • Afficher le Résumé
  • Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) is the main vector of the protozoan Leishmania infantum in Brazil. Its surveillance is carried out by surveillance centers, such as the Zoonosis Surveillance Unit (UVZ) in Natal-RN. In the field, this vector is monitored through collections using the CDC trap. In addition, laboratory maintenance requires blood ingestion, usually from a vertebrate, which can bring contamination risks to the colony and impair the food source. This study aimed to standardize an artificial feeding model for L. longipalpis in a laboratory environment and enable the use of solar energy for the functioning of CDC traps. Feeders commonly used in mosquito colonies were tested. An artificial feeder called Flebfeeding was also constructed, using equipment such as a thermostat, boiler, aquarium pump, plastic hoses, water heating reservoir, and a flat-bottomed flask. The results showed that the FlebFeeding system was efficient in maintaining the blood temperature during feeding and attracting insects, with an average feeding success rate of 53% for females. Engorged females laid an average of 17.49 eggs, and the eggs were viable, with the hatching of larvae and adults in the rearing pots. To evaluate the efficiency of solar charging, a charger was built with a 100W solar panel directly connected to wires attached to the positive and negative terminals of a voltage regulator and the battery. Although the charging took longer than the conventional charger, about 18 hours to complete, with an average current of 0.44A, it was found that the charging was efficient and had a similar field use durability to a conventionally charged battery, which took an average of 10.6 hours to reach full battery charge, with an average current of 1A. The results indicate that artificial feeding through Flebfeeding is effective for L. longipalpis females and offers advantages over conventional feeding, as reducing the use of animals in laboratory experiments is an important ethical principle. As for charging CDC trap batteries with photovoltaic panels, it is a viable option and may potentially generate savings in public expenses. However, further research and cost-benefit analyses are necessary to evaluate the feasibility of the techniques on a larger scale.

7
  • JOSÉ LUCAS ANDRADE
  • EVALUATION OF THE IMMUNOGENIC RESPONSE OF PROTEIN EXTRACTS FROM Plasmodium berghei ANKA AND ITS POTENTIAL PROTECTIVE EFFECT IN EXPERIMENTAL INFECTION

  • Leader : VALTER FERREIRA DE ANDRADE NETO
  • MEMBRES DE LA BANQUE :
  • VALTER FERREIRA DE ANDRADE NETO
  • ISABELA PENNA CERÁVOLO
  • ANDRE TALVANI PEDROSA DA SILVA
  • Data: 31 août 2023


  • Afficher le Résumé
  • Malaria is a parasitic disease with a wide distribution across continents and is an important public health issue worldwide, in view of the high annual rates of morbidity and mortality. Immune responses against Plasmodium are complex and slow, as a protozoan mechanism to attenuate the host's immune processes to infection. Numerous efforts have been employed to understand the ability of plasmodium proteins to modulate a protective immune response in the host in order to reduce the replication of the protozoan in the blood phase and minimize the complications of severe malaria. In this context, this study aimed to analyze the ability of protein extracts obtained from the erythrocytic phase of Plasmodium berghei ANKA to stimulate an effector immune response in the murine model and suppress parasitemia after challenge with 105 infected red blood cells, using the intraperitoneal route. Three cycles of immunizations were performed using the crude extract of P. berghei ANKA, associated with two different adjuvants: aluminum hydroxide and Freund's adjuvant. Furthermore, this immunization scheme was also evaluated in infected mice, subsequently treated and cured, as well as in animals without prior immunity against malaria. Each immunization was performed at an interval of 15 days, in female mice, Mus musculus species, lineage BALB/C, aged between 6 and 8 weeks. 30 days after the last immunization, the animals were challenged with the infecting inoculum and evaluated for parasitemia suppression and humoral immune response. A significant humoral immune response was observed compared to the control group already in the first immunization. With an increase in the production of cytophilic antibodies, IgG2a and IgG2b, for animals previously exposed to malaria. In addition, in these same groups, there was a suppression of parasitemia when compared to the group of non-infected/untreated animals. The experimental group composed of animals previously exposed to the infection and immunized with protein extract/aluminium hydroxide showed total suppression of parasitemia on the 13th day. A similar result was observed in the group immunized with protein extract/Freund's adjuvant, showing a significant reduction in the parasitic load, both in comparison with the control group and in comparison with the non-immunized group, but subjected to successive infections. The results obtained in this study show that the animals immunized with the adjuvants used and, in the condition of previous exposure to the parasite, present high levels of specific antibodies, and, possibly, are less prone to the development of complications of severe malaria, when compared to the non-immunized group and with animals submitted to reinfection, without immunizations.

8
  • FRANCISCO CARLOS VILELA
  • ANIMAL AND HUMAN SPOROTRICHOSIS – AN EPIDEMIOLOGICAL STUDY IN NATAL, RN, BRAZIL.

  • Leader : PAULA RENATA LIMA MACHADO
  • MEMBRES DE LA BANQUE :
  • KLEBER JUVENAL SILVA FARIAS
  • PAULA RENATA LIMA MACHADO
  • VALERIA SORAYA DE FARIAS SALES
  • Data: 31 août 2023


  • Afficher le Résumé
  • Sporotrichosis is a subacute or chronic mycosis caused, in most cases, by traumatic implantation of the fungus Sporothrix sp. in the skin. The disease has become a public health problem in Natal, Rio Grande do Norte (RN), Brazil, due to the significant increase in cases in felines and humans. However, there is still a lack of effective strategic control and prevention measures to contain the advance of this zoonosis that occurs in Natal and RN. The objective of this work was to study, in temporal and spatial terms, the occurrence of animal sporotrichosis and relate the occurrence of recorded human cases, and suggest to public agencies about the need for improvement in intervention for clinical and laboratory diagnosis, prevention and control of this disease. feline infection. From April 2022 to May 2023, 231 exams were performed at the Zoonosis Surveillance Unit in Natal to diagnose sporotrichosis in cats. Being 53% of the positive cases, 16.4% negative and 29.4% inconclusive for sporotrichosis. Two techniques were chosen, cytological and mycological analysis. The main symptoms presented by the examined animals were: skin ulcer, hair loss, sneezing, coughing, nasal pain and panting respiratory effort. Most cases 65.9% were registered in the north area of Natal, followed by the west and south areas. This work contributed to the description of the dynamics of human and animal sporotrichosis in Natal, in terms of space and time, using official notification data. Especially to compare the places of occurrence of human and animal cases, it is possible to discuss their relationships and evident deficiencies in relation to the surveillance of cases in both people, mainly the animal. Compulsory notification of animal cases needs to be more widespread among veterinarians and its flow facilitated and well defined, aiming at greater adherence to this important surveillance measure. In this study we noticed the advance of sporotrichosis in the municipality of Natal, the cases are concentrated in the area of lower purchasing power with configurable environmental conditions the emergence of neglected diseases, It is necessary to implement the Active Epidemiological Surveillance in the city of Natal, due to the increase in human cases and increase in feline cases. And that health education activities remained with the population and professionals from the various areas of animal and human health involved in the dynamics of sporotrichosis in Natal.

2022
Thèses
1
  • ANA BEATRIZ LOURENÇO DE ASSIS
  • Use of ecological modeling to predict the potential geographic distribution of species of family Calliphoridae of forensic importance in the Northeast region of Brazil.

  • Leader : RENATA ANTONACI GAMA
  • MEMBRES DE LA BANQUE :
  • BRUNO CAVALCANTE BELLINI
  • JÉSSICA TEIXEIRA JALES
  • RENATA ANTONACI GAMA
  • Data: 27 avr. 2022


  • Afficher le Résumé
  • Forensic entomology is the science that studies insects and other arthropods related to legal issues. The use of these animals as evidence of crime is dated in the literature since the 13th century and is currently gaining strength in academia and as an auxiliary component in criminal investigations in different parts of the world. In Brazil, entomological research focused on the forensic area has been more and more frequent, but consolidation of forensic entomology in the country still depends on the interaction between academic production and the demand and reality of the judicial police. Brazil is one of the countries with one of the greatest diversities in the world and this will be reflected in the cadaveric fauna, and, together with the peculiarities of national biomes, there is an urgent need for regional entomological studies. The formation of a database and the estimation of potential species distribution can generate important information for forensic entomology, especially in cases of cadaver displacement, data for IPM, in addition to assisting fauna survey research and species identification. The main objective of this work is: to determine the potential distribution of fly species of the Calliphoridae family of forensic interest in the Northeast region of Brazil and to create a database on Calliphoridae fly collecting. For the study, a bibliographic survey was carried out in the portals of national and international journals and data on the collecting and distribution of the flies were obtained, used to generate a database. For an ecological modeling, ArcGis and MaxEnt software were used, which use sets of environmental variables and geographic data to generate the models. The analyzes showed that Chrysomya albiceps and Chrysomya megacephala were the most collected species in the Northeast, with Pernambuco being the state that holds most of the records of animals and works in the area. This research will synthesize biological, taxonomic and ecological information that can help future research and the application of forensic entomology in the judicial sphere.

2
  • BRENDA SANTOS LEITE
  • Incidence of intestinal parasites in cancer patients treated at a referral center in Rio Grande do Norte.

  • Leader : LILIAN GIOTTO ZAROS DE MEDEIROS
  • MEMBRES DE LA BANQUE :
  • HENRIQUE ROCHA DE MEDEIROS
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • LUÍSA MOURÃO DIAS MAGALHÃES
  • Data: 29 avr. 2022


  • Afficher le Résumé
  • Several studies have reported the incidence of opportunistic intestinal parasites in populations of immunosuppressed patients, in patients undergoing hemodialysis, in transplant recipients and in those with autoimmune diseases. However, there is little information about these parasites in cancer patients submitted or not to cancer treatment. Thus, the objective was to evaluate the incidence of intestinal parasites in cancer patients treated at a referral center in Rio Grande do Norte, in order to show whether there is a relationship between their presence, the patient's health status and the completion of cancer treatment, in addition to establishing an association with their social, economic and behavioral characteristics. For this, fresh stool samples were requested from 67 cancer patients aged 18 years or older, who were diagnosed with cancer, undergoing or not to chemotherapy and that signing the “Termo de Consentimento Livre e Esclarecido (TCLE)”. The samples were submitted to the Holfmann, Pons and Janer (HPJ), Willis and Rugai, methods to determine the presence of intestinal parasites and a questionnaire was applied on the socio-economic aspects, food and hygiene habits and chemotherapy and parasitological information of the patients. The collected data were analyzed using descriptive statistics (position measures (median and mode), absolute and relative frequencies), contingency tables and Chi-Square test, in the R ® software [R Core Team (2021)]. The positivity of the stool parasitological exams was 56,7%, with 74.2% being characterized as monoparasitic infection and 25.8% as multiple infection. Endolimax nana (36,8%) and Entamoeba coli (34,2%) were prevalent, followed by Entamoeba histolytica, Giardia lamblia, Hymenolepis spp. and hookworms. The age group most affected by parasitism was 30 to 59 years old, female, and undergoing cancer treatment. Regarding the type of cancer, 87% of parasitized patients had breast cancer concomitant with polyparasitism. There was a relationship between the oncological treatment and the presence of nausea symptoms (p<0.05) in the parasitized patients, however, no association was identified between the parasitism and the other factors evaluated in the questionnaire (p>0.05), concluding that the infection of the patients regardless of whether or not the patient is undergoing cancer treatment and whether he has symptoms such as vomiting and/or diarrhea.

3
  • RAYANE FRANÇA DA SILVA
  • IMPACT OF THE COVID-19 PANDEMIC ON THE PROFILE OF BACTERIAL RESISTANCE IN A HOSPITAL IN THE CITY OF NATAL-RN

  • Leader : MARIA CELESTE NUNES DE MELO
  • MEMBRES DE LA BANQUE :
  • CAIO AUGUSTO MARTINS AIRES
  • JOSE VERISSIMO FERNANDES
  • MARIA CELESTE NUNES DE MELO
  • Data: 25 août 2022


  • Afficher le Résumé
  • The emergency of Covid-19 brought an uncertain epidemiological and sanitary scenario, resulting in an increase in the use of antimicrobials in an empirical way to prevent possible bacterial infections acquired secondary to viral infection in patients hospitalized in ICUs. This study aims to evaluate the impact of the Covid-19 pandemic on the bacterial resistance profile in a private hospital located in the city of Natal-RN. This is a cross-sectional descriptive study of a retrospective nature, in which the resistance profile of bacterial isolates from hospitalized patients was evaluated over a period of three years, covering pre-pandemic and pandemic periods. Through the notification sheets of the Hospital Infection Control Service (SCIH) of the hospital studied, 4,330 (92.3%) positive cultures for bacteria were found in the three years evaluated. Bacterial isolates were equally distributed between female and male patients, with a higher prevalence in blood cultures (31%) and urine cultures (29.3%) and from ICUs (43.2%). Patients with Covid-19 had 12.1% bacterial coinfection. The most prevalent bacteria were Gram negative Klebsiella spp. (26.5%), Pseudomonas spp. (22.4%) and Escherichia coli (11.6%). Among the Gram positive, Staphylococcus spp. were the most prevalent (27.1%). Multi-resistance was observed in 2,767 (63%) of the bacteria with the following prevalence: Acinetobacter spp. 110 (86.8%), Pseudomonas spp. 679 (69.9%), Staphylococcus spp. 960 (81.8%) and Klebsiella spp. 692 (60.1%). There was a statistically significant association (p<0.001) of multidrug resistance with the period of the pandemic (2020-2021) as well as with patients diagnosed with COVID-19 (p=0.001). These results show that COVID-19 may imply a possible increase in the circulation of more resistant bacterial strains in the hospital studied in the years that follow, if more specific control measures are not taken.

4
  • MONALISA SILVA DE SOUZA
  • ANTIMICROBIAL ACTIVITY AND SYNERGIC EFFECT BETWEEN THE SYNTHETIC DERIVATIVE 4-HYDROXYCUMARIN AND AMPHOTERICIN B FRONT SAMPLES OF Candida albicans

  • Leader : VANIA SOUSA ANDRADE
  • MEMBRES DE LA BANQUE :
  • MARIA APARECIDA RESENDE STOIANOFF
  • MARIA CELESTE NUNES DE MELO
  • VANIA SOUSA ANDRADE
  • Data: 26 août 2022


  • Afficher le Résumé
  • Fungal infections have become a major challenge in view of the impact of resistance to usual antifungal agents on therapeutic management, in addition to the lack of implementation of more advanced diagnostic techniques in public health units. Concomitantly, a greater number of cases is observed, reflecting an increase in the incidence of fungal diseases, both in immunosuppression conditions and in immunologically healthy patients in exogenously acquired infectious processes. Species of the genus Candida continue to be the most implicated in invasive fungal infections, which notoriously trigger high mortality rates. Given this scenario, there was an increase in the search for the development of new antifungal agents, as well as the implementation of strategies to prevent candidemia. Studies with bioactive products derived from plants, which historically have well-established biological properties and which demonstrate safety in terms of toxicity, have been evaluated for a more concrete application. Among the compounds under study, coumarins and their derivatives, such as 4-hydroxycoumarin, stand out, which has important attributes, including antiviral, antitumor and anticoagulant properties. In this context, the present study aimed to evaluate the antifungal activity of the synthetic phenolic compound 4-hydroxycoumarin on Candida albicans strains, as well as to analyze the synergistic effects between this compound and the antifungal Amphotericin B. The biological activity of 4-hydroxycoumarin was investigated in 17 samples of Candida albicans, from the determination of the Minimum Inhibitory Concentration (MIC) by the broth microdilution technique and by the Minimum Fungicide Concentration (MFC) method. To verify the synergistic effect, through the Checkerboard method, 5 samples of the C. albicans species were selected, considering pathogenicity criteria among the 17 strains and the MIC values found. Antifungal activity was found for 15 of the 17 strains tested with MIC ranging from 5.0 to 0.625 mg/mL, and CFM ranging from 5.0 to 1.25 mg/mL. Regarding the evaluation of the synergistic effect between the synthetic compound and the antifungal, it was observed that this combination presented an additive and indifferent character against some strains. It was concluded that 4-Hydroxycoumarin has antifungal potential, mostly of a fungistatic nature, that is, it reduces the yeast load, but it was not very effective in relation to the synergistic effect when associated with Amphotericin B, an antifungal recognized as a fungicide, suggesting that 4-Hydroxycoumarin may be a promising strategy in antifungal therapy.

5
  • CLEVERTON DA PAZ MANGABEIRA
  • Study of the clinical characteristics of pregnants with COVID-19 and the immune response of IgG and IgA antibodies against peptides of the SARS-CoV-2 Spike protein.

  • Leader : PAULA RENATA LIMA MACHADO
  • MEMBRES DE LA BANQUE :
  • GUILHERME MARANHAO CHAVES
  • KLEBER JUVENAL SILVA FARIAS
  • PAULA RENATA LIMA MACHADO
  • Data: 30 août 2022


  • Afficher le Résumé
  • The new coronavirus pandemic has challenged health systems all over the world and the entire society. The objective of this study was to describe the clinical and laboratorial profiles of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in hospitalized pregnants of the Januário Cicco Maternity School, Natal, Rio Grande do Norte. The study is prospective in which were included 80 pregnant symptomatic women (median age: 27 years) with suspected COVID-19 and sixteen patients who had had COVID19 earlier in pregnancy and were no longer symptomatic at the time of collection of samples, between the period of march and december of 2021. SARS-CoV-2 detection were made by RT-PCR in samples of naso/oropharyngeal swab and antibodies search were made by enzyme immunoassay (ELISA) which detected IgG and IgA antibodies specific to SARS-CoV-2 Spike protein. Among the 80 symptomatic patients, 40 (50%) were RT-PCR positive and 40 were RT-PCR negative. Sixty-nine sera samples were obtained from the symptomatic pregnants for antibodies detection analysis, 58% (40/69) samples had anti-SARS-CoV-2 IgG or IgA, in which 30,4% were IgG and IgA positive; 11,6% were IgG positive and IgA negative; 15,9% were IgG negative and IgA positive; 42% were IgG and IgA negative. Most frequent symptoms in contaminated women were: cough (52,5%), rhinorrhea (35%), fever (32,5%) and sore throat (27,5%). Eight infected patients needed support ventilation. Acute respiratory distress syndrome were present in 7,5% (3/40) of patients and one died. Average length of stay in the infected population were of 8,3 days. In regard of the newborns, evidence of vertical transmission were not found. These data contribute to a better understanding of the characteristics and the impact of the SARS-CoV-2 infection in pregnancy.

6
  • ÍCARO VALENTIM CÂMARA MAGGI
  • ANTI-INFLAMMATORY ACTIVITY OF KAULERPINE ALKALOID IN A MURINE MODEL OF LPS-INDUCED ACUTE LUNG INJURY.

  • Leader : JANEUSA TRINDADE DE SOUTO
  • MEMBRES DE LA BANQUE :
  • Barbara Viviana de Oliveira Santos
  • JANEUSA TRINDADE DE SOUTO
  • VALTER FERREIRA DE ANDRADE NETO
  • Data: 31 août 2022


  • Afficher le Résumé
  • Natural products present in marine algae of the genus Caulerpa have been the focus of scientific investigation, due to compounds with different pharmacological activities. Therefore, the aim of this work was to evaluate the effect of the alkaloid caulerpine, obtained from marine algae of the genus Caulerpa, in a murine model of acute lung injury (ALI) induced by bacterial lipopolysaccharide (LPS). For this, we evaluated the dose of caulerpine that could best inhibit cell migration to the lung of animals stimulated with LPS. Thus, Swiss mice were treated orally with different doses of calerpine (4 mg/kg and 2 mg/kg) or with dexamethasone (1 mg/kg) intraperitoneally. One hour after this treatment schedule, the animals received LPS (167 µg/ml) intranasally. After 24 hours, bronchoalveolar lavage (BAL) was obtained to determine the total number of leukocytes. The treatment of mice with caulerpine, at the two doses tested, reduced the migration of leukocytes to the lung cavity in a similar way. In view of these results, the dose of 2 mg/kg was used for the other analyses. The same procedure was repeated to evaluate cell migration kinetics, differential cell counts, determination of total protein and pro-inflammatory cytokine levels in BAL, and histological analysis of the lung at 6, 24 or 48 hours. Caulerpine inhibited the migration of leukocytes to the lung in the ALI model at the three times analyzed, with a significant decrease in the migration of polymorphonuclear cells. It is possible to correlate this effect with the decrease in plasma proteins and pro-inflammatory cytokines IL-6, IL-12 and TNF-α in BAL, especially at 24 and 48 hours. Finally, the inhibition of the inflammatory process colud be followed by a decrease in tissue damage observed in the lungs of animals treated with caulerpine, where there is a lower inflammatory infiltrate, with less edema, similar to what is observed in the lungs of animals treated with dexamethasone. It is concluded that treatment with caulerpine, at a dose of 2 mg/kg, was effective in reducing the inflammatory process in the lung in a murine model of acute lung injury.

2021
Thèses
1
  • ANA CLARA OLIVEIRA DA SILVA
  • Tripanosomatide infection in chiropters captured in the city of Natal-RN

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • ANTONIA CLAUDIA JACOME DA CAMARA
  • GIRLEY FRANCISCO MACHADO DE ASSIS
  • PAULO MARCOS DA MATTA GUEDES
  • RENATA ANTONACI GAMA
  • Data: 25 févr. 2021


  • Afficher le Résumé
  • Trypanosomatids are parasite protozoa belonging to the Trypanosomatidae family, which inhere Trypanosoma cruzi and Leishmania infantum, etiologic parasites of Chagas disease and visceral leishmaniasis. Chiropterans are ancient and natural hosts of T. cruzi and L. infantum. However, the participation of bats in the epidemiological cycle of T. cruzi and L. infantum is still underestimated. The aim of this study was to evaluate the infection rate by trypanosomatids in chiropterans captured in the city of Natal-RN. Ninety-five bats were collected together with Zoonosis Control Center (CCZ) of Natal between 2019 and 2020, 50 animals from passive capture in urban areas and 45 obtained from active capture, using fog nets, in an environmental preservation area (Parque das Dunas) in the municipality of Natal-RN. Animals were identified, euthanized, and fragments of liver, spleen and skin were collected. DNA was extracted and PCR for T. cruzi kDNA and HSP70 which detects the Leishmania genus were performed. The species Artibeus planirostris was the most prevalent among bats captured in urban environments and Parque das Dunas, presented 95% and 56% of infection by Leishmania spp and T. cruzi, respectively. The total rate of bats infection by T. cruzi, Leishmania spp, and co-infected T. cruzi/Leishmania was 56% (53/95), 77% (69/95) and 41% (39/95), respectively. Leishmania spp was detected into the skin of 55% (43/79) of animals evaluated. Animals captured in Parque das Dunas showed a higher rate of infection by the evaluated parasites (91%), when compared to bats captured in the urban environment (84%). Fruit-eating chiropterans (90%) had a higher rate of infection by Leishmania, when compared to insectivores (65%) and nectarivores (33%). On the other hand, nectarivorous bats (83%) had a higher infection with T. cruzi, when compared to frugivorous (56%) and insectivorous (52%).  Our results demonstrate a high infection rate by trypanosomatids in chiropterans captured in the city of Natal-RN, indicating that they could participate in the epidemiological transmission cycle of Leishmania and T. cruzi.


2
  • VICTORIA RAMÍREZ OROZCO
  • Evaluation of Biomarkers for chronic chagasic cardiopathy 

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • ANTONIA CLAUDIA JACOME DA CAMARA
  • JONILSON BERLINK LIMA
  • PAULO MARCOS DA MATTA GUEDES
  • Data: 26 févr. 2021


  • Afficher le Résumé
  • Chronic chagasic cardiopathy is observed in 17-50% of patients, contributing to morbidity and mortality of Chagas disease. There are no biomarkers of clinical evolution described for Chagas disease, requiring high-cost clinical examinations (electrocardiogram, echocardiogram, X-ray, opaque enema), which require the presence of specialized professionals and many cases are invasive and uncomfortable to patients. CTLA-4 is expressed in regulatory T cells and is soluble in blood plasma, CTLA-4 expression is influenced by infections. Cardiac proteins released after tissue damage can generate the production of autoantibodies that contribute to the process of cardiac damage. This study aimed to evaluate anti-troponin I, anti-myoglobin autoantibodies and CTLA-4 soluble in serum as non-invasive clinical biomarkers for chronic chagasic cardiophaty. Specific IgG production anti-T. cruzi and autoantibodies anti-troponin I, anti-myoglobin, and soluble CTLA-4 concentration were determined by ELISA in the serum of 90 chronic chagasic patients with indeterminate (n = 30), cardiac (n = 30), digestive (n = 14) and cardiodigestive (n = 16) clinical forms, and correlated with left ventricular ejection fraction, cardiothoracic index and atrial diameter. Sera from uninfected individuals (n = 30) were used as controls. Chagasic patients with indeterminate, cardiac, digestive and cardiodigestive clinical forms had higher production of total IgG anti-T cruzi and anti-troponin I and anti-myoglobin autoantibodies, when compared to uninfected individuals. In addition, cardiac patients showed higher production of anti-myoglobin IgG autoantibodies compared to indeterminate patients. Interestingly, indeterminate patients had higher production of soluble CTLA-4, when compared to patients with cardiac, digestive and cardiodigestive forms. Our results indicate that the high production of anti-troponin-I and anti-myoglobin autoantibodies associated with low soluble CTLA-4 may be associated with the development of the cardiac form of Chagas disease.

3
  • CARLIKELLY GLEICY DA SILVA
  • EVALUATION OF NATURALLY INFECTED GOATS BY GASTRINTESTINAL PARPARASITES IN CONFINING REGIME

  • Leader : LILIAN GIOTTO ZAROS DE MEDEIROS
  • MEMBRES DE LA BANQUE :
  • MARIA ROSALBA MOREIRA DAS NEVES
  • GELSON DOS SANTOS DIFANTE
  • HENRIQUE ROCHA DE MEDEIROS
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • VALTER FERREIRA DE ANDRADE NETO
  • Data: 20 avr. 2021


  • Afficher le Résumé
  • Goat farming is a worldwide activity and in Brazil it has an important role in the economy. Unfortunately, the production of these animals has been impared by problems associated with gastrointestinal parasites that affect the herd development and consequently generate economic losses. To control parasitic infections, alternative strategies to the use of anthelmintics are being thought. In this sense, the aim of this work was to evaluate the response of male goats to natural infections by gastrointestinal parasites kept in an confining regime and fed with two sources of dietary fiber. For this, 24 goats, without defined breed standard, castrated and not castrated, fed with palm and sugarcane bagasse and elephant grass palm and hay, were evaluated weekly, for 120 days, by counting eggs (EPG) and oocysts (oOPG) per gram of feces, weight, packed cell volume (PCV) and feces culture. After this period, three animals from each treatment were randomly slaughtered and necropsied to collect the gastrointestinal tract and recover the adult parasites. There was no significant difference (p> 0.05) regarding to phenotypic characteristics evaluated. The number of effector cells of the immune response also did not show any significant difference (p> 0.05). Six distinct species of Eimeria spp. were identified, being E. ninakohlyakimovae the prevalent one (29%). A total of 1.251 adult parasites were recovered, with prevalence for Oesophagostomum spp. (1.210), followed by Haemonchus spp. (30), Trichostrongylus spp. (7) and Trichuris spp. (4).  The animals fed with palm and elephant grass hay, regardless of castrated or not castrated, had highest parasitic load, which reinforces the importance of understanding variations in animal management and how it can interfere with endoparasite infection in goats.

4
  • JUCÉLIA ROSSANA DE MEDEIROS
  • Sanitary implications of diptera (Diptera: Muscomorpha) associated with street fairs in the town of Toritama - PE

  • Leader : TACIANO DE MOURA BARBOSA
  • MEMBRES DE LA BANQUE :
  • CARLA DE LIMA BICHO
  • MAGNO AUGUSTO ZAZÁ BORGES
  • TACIANO DE MOURA BARBOSA
  • Data: 25 mai 2021


  • Afficher le Résumé
  • The order Diptera is a diverse group of insects, has a cosmopolitan distribution and a high capacity to colonize different types of environments. In this order, there are muscoid representatives who have a high capacity to adapt to the environment modified by humans - synanthropic environments. Additionally, they have high medical and sanitary relevance, since they are related to the transmission of pathogens. In this context, it is known that anthropic environments (e.g. street fairs) with high human circulation and food supply can directly influence the composition and structure of muscoid dipterans assemblies, since it provides the facilities offers and abundance of food for flies. Therefore, the objective of this work was to evaluate the effect of street fairs on the composition and structure of assemblies of flies with sanitary importance in an urban environment in Toritama - Pernambuco. In addition, the presence of helminth eggs and protozoan cysts on the body surface of these flies was also analyzed. To this end, collections of dipterans were carried out in 2 commercial spots in the town of Toritama, and the flies were captured from traps called “Ratoeira Adesiva Cola Rato” before and after the occurrence of street fairs. In the parasitological analysis, the HPJ method was performed. Throughout the study, 7.780 specimens belonging to six Diptera families and a wealth of 14 species were collected. Muscidae was the most representative family with 96.98% of all flies caught, followed by Sarcophagidae (1.99%), Phoridae (0.53%), Calliphoridae (0.42%), Fanniidae (0.03%) and Stratiomyidae (0.02%). Muscidae was also the most diverse taxon with the registration of four species, followed by Sarcophagidae (3 spp.), Calliphoridae (2 spp.), Phoridae (2 spp.), Stratiomyidae (2 spp.) and Fanniidae (1 spp.). In addition, it was observed that the presence of the street fair influenced the structure of the assemblies, which was more abundant after the occurrence of the street fairs (H = 6.56; df = 1; P <0.01). Regarding the composition of the assemblies, 50% of the species were common to both treatments, with emphasis on the dominant species Musca domestica and Megaselia scalaris. No parasitological structures were found in the analyzed samples. Thus, the contribution of this study reveal that street fairs can modulate the structure and composition of fly assemblies in urban environments, including be able to offer health risks, considering that species of medical importance are related to this environment.

5
  • KARINE LILIAN DE SOUZA
    1. Título do trabalho: Monitoring of cytomegalovirus infection in patients who underwent hematopoietic stem cell transplantation.
  • Leader : PAULA RENATA LIMA MACHADO
  • MEMBRES DE LA BANQUE :
  • IVANISE MARINA MORETTI REBECCHI
  • KLEBER JUVENAL SILVA FARIAS
  • PAULA RENATA LIMA MACHADO
  • Data: 31 mai 2021


  • Afficher le Résumé
  • Primary infection or reactivation by cytomegalovirus (CMV) is associated with increased rates of morbidity and mortality in patients who undergo hematopoietic stem cell transplantation (HSCT). CMV infection increases risk for other opportunistic infections, graft loss and development of graft versus host disease, due to indirect effects on the immune system. Thus, the aim of the study was to estimate the rate of CMV infection up to 90 days after transplantation in patients who underwent HSCT and to relate it to clinical characteristics. Serum samples were collected fortnightly up to 90 days after transplantation from patients seen at Hospital Rio Grande, Natal, Rio Grande do Norte. The extraction of viral DNA was performed in columns with silica gel membrane, according to the manufacturer's instructions, and CMV detection was performed by nested-PCR, using specific primes. 214 peripheral blood samples were collected from 51 patients. Of these, 60.8% were male and 39.2% female, with a median of 35 years of age. Of the hematological diseases, 29.4% had Acute Myeloid Leukemia (AML), 21.5% Acute Lymphoid Leukemia (ALL), 13.7% Chronic Myeloid Leukemia (CML), 13.7% Lymphomas and 21.5% other diseases hematological. Related allogeneic transplantation was performed in 56.9%, unrelated in 41.2% and autologous in 1.9% of cases. In monitoring CMV infection, it was observed that 15 days post-transplant (D15), 28.6% of the patients were positive, 19.5% in the 30 days post-transplant (D30), 29.3% in the 45 days post-transplant -transplantation (D45), 30.8% in the 60 days after transplantation (D60), 33.3% 75 days after transplantation (D75), and 21.9% in the 90 days after transplantation (D90). This study reinforces the importance of monitoring the early reactivation of CMV in groups of patients undergoing HSCT and that patients who underwent unrelated allogeneic transplantation had earlier viral reactivation compared to related allogeneic transplantation.
    Keywords: cytomegalovirus, hematopoietic stem cell transplantation, PCR.

6
  • MARTHA LAYSLA RAMOS DA SILVA
  • ANALYSIS OF THE EPIDEMIOLOGICAL SITUATION OF DENGUE FOR THE PERIOD 2016 TO 2020 IN THE STATE OF RIO GRANDE DO NORTE, BRAZIL.

  • Leader : JOSE VERISSIMO FERNANDES
  • MEMBRES DE LA BANQUE :
  • JOSE VERISSIMO FERNANDES
  • JOSELIO MARIA GALVAO DE ARAUJO
  • THALES ALLYRIO ARAUJO DE MEDEIROS FERNANDES
  • Data: 31 mai 2021


  • Afficher le Résumé
  • Dengue is an acute and systemic febrile illness, which can have a wide clinical spectrum, ranging from asymptomatic to severe cases. It is an arboviruses transmitted mainly by the Aedes aegypti mosquito, being one of the most important health problems worldwide, causing annual epidemics mainly in tropical and subtropical regions, putting almost a third of the planet's population at risk. The present work presents an analysis of a time series and spatial distribution of dengue cases in the State of Rio Grande do Norte. Secondary data from dengue cases notified in the period between 2016 and 2020 were used, available in the Information System for Notifiable Diseases and Epidemiological Bulletins of the State Department of Health, in the respective years. The analysis was carried out using descriptive statistics. During the time series, a total of 152,628 probable cases of Dengue were reported, with 37,489 cases being confirmed, representing 24.56%. The health region VII, which corresponds to the Metropolitan region of Natal, had the largest number of confirmed cases, totaling 17,654 cases, representing approximately 47.09% of the number of confirmed cases in the state during the period studied. Regarding sociodemographic characterization, females had a higher prevalence of the disease and the most affected age group is between 20 and 49 years old. Dengue in this period studied, showed an epidemiological behavior that characterizes an endemic profile, which can be directly related to the state's infrastructure problems. Our data show an apparent reduction in the number of dengue cases reported in the year 2020. It is possible that this reduction in the number of cases of the disease was due to underreporting, since all the efforts of the health services were focused on coping with the disease. current Coronavirus pandemic (COVID-19).

7
  • JONAI PACHECO DIAS
  • EPIDEMIOLOGICAL AND MOLECULAR ASPECTS OF CHIKUNGUNYA VIRUS IN RIO GRANDE DO NORTE IN 2019 AND 2020.

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • JOSE VERISSIMO FERNANDES
  • JOSELIO MARIA GALVAO DE ARAUJO
  • Maria do Desterro Soares Brandão Nascimento
  • Data: 31 mai 2021


  • Afficher le Résumé
  • Once considered a third world disease, chikungunya fever is currently one of the main emerging arboviruses globally. Shortly after having been established as an autochthonous disease in Brazil, CHIKV spread in the State of Rio Grande do Norte, where it has caused recurrent outbreaks. Because it is a multicausal disease, several factors may be favouring the constant viral circulation within the state. The chronic and disabling aspect of the infection represents one of the main concerns in the field of public health. In this study, samples from several municipalities in Rio Grande do Norte, including the capital city of Natal, were assessed. The epicentre of the cases in the state was also checked, as well as factors that may contribute to the phenomenon of virus spread. A total of 540 samples were tested using qRT-PCR technique, of which 134 were chikungunya positive in the years 2019 (n = 82) and 2020 (n = 52), representing a total of 25% in both years. The largest affected population was the female group (60%) in the age group between 20 and 39 years old. The most frequent complaints presented were fever (65%), headache (48%) and arthralgia (47%). The highest viral load was found on day 0 of the infection. Among the percentages obtained, two probable cases of congenital chikungunya were found, among the newborn group (1.5%) and two asymptomatic cases (1.5%). The highest proportion of cases in 2019 was observed in Natal, in the Tirol neighborhood (57%); and in 2020, in the municipality of Guamaré (33%). The trend of the epicenter of these cases was found in a region close to the Metropolitan Region of Natal (p>0,001). This study contributes to the CHIKF population-based surveillance in Rio Grande do Norte, outlining higher incidence areas, which is useful as a basis for the CHIKV epidemiological control.

2020
Thèses
1
  • ANTONIO CARLOS VITAL JÚNIOR
  • ANTIFUNGAL AND ANTIBIOFILM ACTIVITY OF THE EXTRACT FRACTION Ag2 FROM Agelas dispar AGAINST SPECIES OF Candida

  • Leader : VANIA SOUSA ANDRADE
  • MEMBRES DE LA BANQUE :
  • VANIA SOUSA ANDRADE
  • JOSE VERISSIMO FERNANDES
  • MARIA APARECIDA RESENDE STOIANOFF
  • Data: 11 févr. 2020


  • Afficher le Résumé
  • Microbial biofilms are especially a structural barrier that hinders antimicrobial therapy, having a relevant role in human diseases. For yeasts of the genus Candida, biofilms are characterized as a complex three-dimensional arrangement, consisting of different cellular forms incorporated into an extracellular polymeric matrix. In the last decades, the search for natural compounds for the treatment of microbial infections has grown in order to find new alternatives to the mechanisms of resistance to antimicrobials, including those induced by biofilms. The marine sponge, Agelas dispar (Family Agelasidae) has been mentioned as a bioactive source, due to its secondary metabolites, the alkaloid derivatives, which have significant pharmacological activity. In this context, the aim of this study was to determine the antifungal and antibiofilm activity of the Ag2 fraction of the A. dispar metanolic extract on biofilm-producing strains of the genus Candida. The biological activity was investigated in 13 species of Candida, evaluating the Minimum Inhibitory Concentration (MIC) by the broth microdilution technique and by the Minimum Fungicide Concentration (MFC) method. The quantification of biofilm formation and antibiofilm activity of the Ag2 fraction of the extract was performed with the violet crystal and the obtained optical densities were measured. The Ag2 fraction of the extract showed antifungal activity for 13 strains, demonstrating MIC’s ranging between 2.5 mg/mL and 0.1562 mg/mL. The compound analyzed showed MFC's ranging from 5.0 mg/mL to 0.3125 mg/mL against Candida spp. The results of biofilm formation indicated that all strains were biofilm producers. 10 (77%) weak producing strains and 3 (23%) moderate producing strains. The species C. krusei, C. glabrata and C. parapsilosis were used to test the antibiofilm activity (in formation and mature), resulting in inhibition of cell viability (IC50%) ranging from 49.6% to 57.6%, 70.5% to 76.3% (biofilm in formation) and 42.8% to 67.2%, 63.3% to 78.8% (mature biofilm) when these strains were post-treated, respectively, with the concentrations of 1.25 mg/mL and 2.5 mg/mL of the Ag2 fraction. The Scanning Electron Microscopy (SEM), allowed the visualization of the antibiofilm activity, quantitative decrease of the microbial community, structural alteration and destruction at the cellular level in both the forming and mature biofilms. It was concluded that the Ag2 fraction of A. dispar methanolic extract showed antifungal activity and fungicide nature, in addition to being efficient in decreasing the microbial load and providing changes in the structural morphology of the yeasts involved in the biofilm, as observed in the SEM. Such findings suggest that the mechanism of action of the fraction is at the level of plasma membrane and/or cell wall, inferring that the Ag2 fraction may be a promising antifungal and antibiofilm therapeutic strategy against the different species of Candida.

2
  • FRANCILENE MARIA MOREIRA DE LIMA
  • Cimex lectularius (HETEROPTERA: CIMICIDAE): INVESTIGATION, DISSEMINATION AND FIRST REGISTRATION FOR NORTHEAST BRAZIL

  • Leader : RENATA ANTONACI GAMA
  • MEMBRES DE LA BANQUE :
  • RENATA ANTONACI GAMA
  • PAULO MARCOS DA MATTA GUEDES
  • TATIANA MINGOTE FERREIRA DE ÁZARA
  • Data: 28 févr. 2020


  • Afficher le Résumé
  • The insects of the family Cimicidae (Hemiptera: Heteroptera) are known by the generic name of 'bedbugs'’. These ectoparasites arouse great interest in public health due to their high anthropophilia, and the species Cimex lectularius and Cimex hemipterus are of major medical importance. In recent years, several authors have reported the resurgence of these insects in various parts of the world, probably due to the increase of international tourism and their resistance to most existing insecticides. The objective of this project was to survey the species of Cimicidae present in the city of Natal / RN. A retrospective study of the presence of scimicids was carried out in the municipality from 2010 to 2019 through service orders granted by the pest control companies of the city. Current cases were also searched, in partnership with the zoonosis control center, in strategic locations such as shelters and public shelters in the city. Hotels and inns were also visited and finally the people involved in the study were raised, technicians from the zoonosis control center and pest control companies. The species found was C. lectularius with infestations present in two hotels located in the south of the city and in an apartment in the west of the city. The foster homes were not infested. Five service orders were assigned by four companies. The training was conducted through a lecture with presentation of educational booklet that were made available to participants electronically. This study made the first record of cimicidae in the municipality of natal, thus contributing to studies of the prevalence of these insects in the country, which are currently few and provided data on the biology, behavior and epidemiology of these insects in Natal.

3
  • WILO VICTOR DOS SANTOS
  • METALOPROTEINASES MATRIX EVALUATION IN DOGS NATURALLY INFECTED WITH Leishmania infantum

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • JANEUSA TRINDADE DE SOUTO
  • JONILSON BERLINK LIMA
  • PAULO MARCOS DA MATTA GUEDES
  • Data: 28 févr. 2020


  • Afficher le Résumé
  • Dogs are the primary reservoir of Leishmania parasites. The immune response induced by Leishmania infantum is important for resistance to infection and pathogenesis in these animals. Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that play multiple roles in immune response and matrix remodeling. However, the action of these enzymes may lead to immunopathology in an infectious process that causes host morbidity or mortality and favors the spread of the pathogen and its persistence. Few studies demonstrate the correlation between MMPs expression and clinical manifestations of animals with canine visceral leishmaniasis (CVL). Thus, the objective of this study was to evaluate the expression of matrix metalloproteinases in dogs naturally infected with Leishmania infantum and correlate them to the clinical manifestations of the disease (asymptomatic, oligosymptomatic, symptomatic). For this, the relative expression of MMPs, their tissue inhibitors (TIMPs) and the cytokines, IL-10 and TNF-α, was analyzed in the liver tissue of dogs by real time PCR. The inflammatory process and cytostructure of the liver tissue were measured using hematoxylin-eosin staining. Only symptomatic and oligosymptomatic dogs showed greater expression of MMP-2, when compared to the group of asymptomatic dogs. In addition, oligosymptomatic dogs showed a larger and more diffuse hepatic inflammatory infiltrate when compared to asymptomatic and symptomatic dogs. On the other hand, all dogs infected with L. infantum showed higher expression of tissue inhibitors TIMP-1 and TIMP-4 when compared to uninfected animals. Further more, symptomatic dogs showed a greater expression of the cytokine IL-10 when compared to the other groups. The results demonstrate high expression of MMPs (MMP2, MMP3, MMP9, MMP11 and MMP13) and TIMPs (TIMP1 and TIMP4) is positively correlated with a higher expression of the cytokines TNF-α and IL10 in dogs naturally infected by L. infantum, contributing to destruction of the liver parenchyma and for the clinical evolution of visceral leishmaniasis in animals.

4
  • HENRIQUE RAFAEL PONTES FERREIRA
  • REPELLENT EVALUATION OF ICARIDINE APPLIED ON COTTON FABRIC ON Lutzomyia longipalpis (LUTZ & NEIVA, 1912)

  • Leader : RENATA ANTONACI GAMA
  • MEMBRES DE LA BANQUE :
  • JOSE HERIBERTO OLIVEIRA DO NASCIMENTO
  • RENATA ANTONACI GAMA
  • VERUSKA CAVALCANTI BARROS
  • Data: 2 mars 2020


  • Afficher le Résumé
  • Lutzomyia longipalpis (Lutz & Neiva, 1912) (Diptera: Psychodidae) is the main insect vector of the protozoan Leishmania infantum (ROSS, 1903), the etiological agent of Visceral Leishmaniasis (LV) or Calazar in Brazil. For vector control, the use of insecticidal chemical compounds is highlighted, however, the use of this strategy has been causing resistance in populations of L. longipalpis, considerably increasing the cases of VL in recent years in Brazil. Approaches using repellent materials are considered new alternative to prevent the bite of this insect. Icaridine is one of the approved compounds for the production of insect repellent products in Brazil and other countries since 2009. Therefore, the aim of this study was to evaluate the repellent action of different concentrations (5%, 10%, 25% e 50%) of Icaridine emulsion applied on cotton fabric on adults of L longipalpis. For this, the experiment had the participation of 8 volunteers (4 men and 4 women), and the use of 15 females and 15 males of L. longipalpis evaluated according to the method “Arm in cage”. The different concentrations of Icaridine were elaborated by dilution in 98% alcohol in the Textile Engineering Laboratory of UFRN. The evaluations in each volunteer included the absence of Icaridine (control group) and presence in different concentrations, which occurred within 144 hours and test after washing the cotton fabric with Icaridine that donned the forearm of the volunteers. The results observed in this study reveal that the average landings decreased significantly when the trials involved the Icaridine. It was possible to observe a significant difference (p <0.05) in the average of landings in analyzes from the sex of the volunteer at concentrations of 5% (men = 13.29; women = 8.63) and 10% (men = 8, 72; women = 4.53) with a decrease in this difference at the highest concentrations (25% and 50%). The total protection time (TPT) in the highest concentrations, 25 and 50%, the medians of TPT were higher compared to the lowest concentrations, with 120 and 144 hours, respectively. The average total of landings in the seven repetitions, including the post-wash test, differed significantly (p <0.05) from the control test and Icaridine concentrations, where the average control landing was 52.74 and in different concentrations was 10,03; 6.62; 1.02; 0.27 for 5, 10, 25 and 50%, respectively. Repetition Rate (%R) of 100% was observed for all concentrations within the first 1 hour of testing. Posteriorly, this repellency rate was maintained only at 25 and 50% concentrations until 72 and 96 hours after impregnation, respectively. The% R above 95% (ED95) in the daily tests and after washing the impregnated tissue, served to mark a Minimum Effective Concentration (MEC), where it was observed that 25% of Icaridine maintained %R> 95% providing protection from 98.18%. With this study, it was possible to define that different concentrations of Icaridine were efficient as repellent, varying in relation to TPT and % R. The concentration of 25% was the EMF, presenting better efficiency in the tests presenting relevant results for L. longipalpis repellency, which may become an efficient strategy for the control of bites of this insect vector.
    Fabric Textile. Sand Flies. Behavior. Visceral Leishmaniasis. 

5
  • NATHAN RAVI MEDEIROS HONORATO
  • EVALUATION OF THE PRESENCE OF TRIATOMIES AND DISTRIBUTION OF DTUs from Trypanosoma cruzi IN DIFFERENT MESOREGIONS OF RIO GRANDE DO NORTE, BRAZIL

  • Leader : ANTONIA CLAUDIA JACOME DA CAMARA
  • MEMBRES DE LA BANQUE :
  • ANTONIA CLAUDIA JACOME DA CAMARA
  • DANIELLA CASTANHEIRA BARTHOLOMEU
  • RENATA ANTONACI GAMA
  • Data: 3 mars 2020


  • Afficher le Résumé
  • This work evaluated the presence of triatomines, entomological indicators of triatomines and the distribution of the different typing units (DTUs, from the English Discrete Typing Units) of Trypanosoma cruzi isolated from vectors in different mesoregions of the state of Rio Grande do Norte (RN). The captures of the triatomines were carried out in rural areas of 21 municipalities, 71 rural communities and 345 household units in the period from 2015 to 2019. Genotyping was carried out using the mitochondrial gene markers of subunit 2 of cytochrome oxidase (CO II), divergent domain D71 of the 24Sα gene of the ribosomal DNA (rDNA) and intergenic spacer of the miniexon genes (SL-IR). Infestation was observed in 7.5% (26/345) of households and in 16.2% (56/345) of the surveyed households. The presence of nymphs in the home was observed in 94.7% (45/47) of the infested home units, indicating colonization in this environment. A total of 1,084 triatomines were captured in anthropic and wild environments, with Triatoma brasiliensis (84.5%) being the most found species, followed by Triatoma pseudomaculata (14.9%), Panstrongylus lutzi (0.5%), Rhodnius nasutus (0.1%). T. brasiliensis was the only species found in the home, peridomicile and wild environment, and also infested the largest variety of ecotopes. Natural infection by T. cruzi was observed in 11% (96/872) triatomines, with T. brasiliensis being the species with the highest number of specimens (95.0%). Of the 96 infected triatomines, it was possible to isolate the parasite from 33 (34.4%) samples, all of which were genotyped. DTU I: haplotype A in COII, rDNA 2 and SL-IR with 150 bp was identified in 51.5% (17/33) of the parasite isolates in the peridomicile infecting T. brasiliensis in the three studied mesoregions, and T. pseudomaculata, in West mesoregion. DTU II: haplotype C in COII, rDNA 1, SL-IR with 150 bp was observed in 9.1% (3/33) of isolates in T. brasiliensis only in the Central mesoregion both in the home and in the wild. While DTU III: haplotype B in COII, rDNA 2, SL-IR with 200 bp was in 27.3% (9/33) of the samples and was also found in all mesoregions, in the home area in the West mesoregion, in wild areas in the Center and peridomicile in Agreste. The proximity and even overlap of areas of occurrence of the genotypes was observed in several areas. In Caicó, this fact was evidenced by the detection of mixed infections with TcI and TcII. These results show that T. brasiliensis remains the species with the greatest epidemiological importance in the semi-arid region of RN, due to its wide geographical distribution, high degree of adaptation to different environments, ecotopes, high rate of natural infection by T. cruzi and the diversity of DTUs, and reinforce the need for continuous entomological surveillance in the state, in order to prevent contact of this species with humans and domestic animals.

6
  • JORGE LUCAS NASCIMENTO SOUZA
  • EVALUATION OF THE IMMUNOLOGICAL RESPONSE IN THE GASTRINTESTINAL TREATMENT OF RESISTANT SHEEP SUSTAINABLE TO NEMATOID INFECTIONS

  • Leader : LILIAN GIOTTO ZAROS DE MEDEIROS
  • MEMBRES DE LA BANQUE :
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • VALTER FERREIRA DE ANDRADE NETO
  • LILIAN LACERDA BUENO
  • Data: 6 mars 2020


  • Afficher le Résumé
  • Gastrointestinal nematodes infections cause losses in animal production, especially in sheep farming, an important socioeconomic activity. Undestanding the mechanisms involved in the immune response of these animals to these infections is essential, onceanimals belonging to the same breed and herd respond differently to infections. The lack of studies involving this differentiation in sheep leads to the need to better understand these mechanisms. In this sense the aim of this work was to quantify the expression of cytokines (IFN-γ, TNF-α, IL-4, IL-13, IL-9, IL-22, TGF-β and IL-10) involved in the immune response to gastrointestinal nematodes in sheep phenotypically classified as resistant and susceptible. Abomasum (AB), small (SI) and large intestine (LG) samples from 12 crossbred sheep ½ Santa Inês x ½ without a defined breed pattern, naturally infected by gastrointestinal nematodes, 6 resistant (RG) and 6 susceptible (SG) animals were used. From these samples, total RNA was isolated from the tissues, cDNA synthesized and quantified by qPCR to determine relative expression, using na ideal reference gene for each tissue (GAPDH, SDHA and RPL-19) and analyzed by REST© software. There was increase in the expression of IL-4 and IL-13 in all tissues of gastrointestinal tract in resistant group to nematode infections compared to animals in the susceptible group. In AB, SI and LG, respectively, there was an increase of 2,5x, 2,8x (p<0,017) and 1,3x of IL-4 and 1,1x, 1,3x and 7,0x (p<0,001) of IL-13. On the other hand, TNF-α and IL-9 were associated with the profile of susceptibility to infection, being more expressed in all gastrointestinal segments of susceptible animals. In this group, AB, SI and LG there was increase of 1,1x, 5,3 (p<0,001) and 2,2x of TNF-α and 2,6x, 1,8x and 1,5x of IL-9. In conclusion, IL-4 and IL-13 were associated with resistance profile and TNF-α and IL-9 with susceptibility profile. The expression of the other cytokines varied in the different organs of the different groups due to the dynamics of parasites in each organ being different.

7
  • ANA BEATRIZ RAMALHO DA SILVA
  • EFFECT OF THE CAULERPINE INDOLIC ALKALOID IN THE MURINE MODELS OF PERITONITIS AND EAR EDEMA

  • Leader : JANEUSA TRINDADE DE SOUTO
  • MEMBRES DE LA BANQUE :
  • JANEUSA TRINDADE DE SOUTO
  • VANIA SOUSA ANDRADE
  • ADEMILSON PANUNTO-CASTELO
  • Data: 28 août 2020


  • Afficher le Résumé
  • The appearance of adverse effects associated with the use of corticosteroids has driven the search for compounds derived from natural products with pharmacological properties, so that they can be used as an alternative anti-inflammatory therapy. Thus, the present study sought to assess the anti-inflammatory potential of caulerpin (CLP), an alkaloid derived from algae of the genus Caulerpa, in murine models of zimosan-induced peritonitis and xylene-induced ear edema. For this, mice were orally treated with CLP at doses of 4, 2, 1 mg/kg and dexamethasone (1 mg/kg) and 1 hour later received zymosan (40 mg/kg) intraperitoneally. After 24 hours, the peritoneal lavage was collected and the number of cells was determined. The same procedure was repeated to assess the migration kinetics and presence of cytokines in the peritoneal lavage at 6, 24 and 48 hours. For ear edema, the animals were treated with the same different doses of CLP, followed by topical application of xylene (40μL) in the right ears, 1 hour after treatment. The level of edema was determined by calculating the percentage of inhibition and morphological analysis evaluated in equal sections of the removed ears. In the peritonitis model, pre-treatment with CLP was able to reduce cell migration to the peritoneal cavity at doses of 4, 2 and 1 mg/kg, with the dose of 2 mg/kg being the one that showed results more expressive in inhibiting cell migration. In the cell migration kinetics, using the dose of 2 mg/kg of CLP, it was observed that this dose was able to inhibit this event in the three evaluated times, after the administration of zymosan. And this effect was related to the decrease in the levels of IL-1β and IL-6 in the peritoneal lavage, during the studied kinetics. In the edema model, it was observed that the three CLP doses tested acted by inhibiting the formation of xylene-induced ear edema, which was demonstrated by the high percentages of inhibition of the process (based on the weight of the ear sections) and in the preservation of the tissue morphology of the ears in the animals that received the treatments, with the dose of 2 mg/kg being the most efficient in this inhibition. Thus, the CLP has anti-inflammatory activity in the models studied and the dose of 2 mg/kg is an optimal dose in controlling inflammation in the models of peritonitis induced by zimosan and xylol-induced ear edema.

2019
Thèses
1
  • ROGÉRIO DAMIÃO DE SOUZA JÚNIOR
  • Evaluation of the FAMACHA © method for the detection of Haemonchus contortus anemia in goats and sheep

  • Leader : LILIAN GIOTTO ZAROS DE MEDEIROS
  • MEMBRES DE LA BANQUE :
  • ANA CARLA DIÓGENES SUASSUNA BEZERRA
  • ANTONIA CLAUDIA JACOME DA CAMARA
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • Data: 26 févr. 2019


  • Afficher le Résumé
  • In Brazil, mainly in the semi-arid regions, the control of the gastrointestinal parasites is carried out by the use of anthelmintics, although in many cases without considering the recommendations, leading to the development of parasitic resistance. Thus, the FAMACHA© method was introduced as a new technique to aid in the parasites control by means of the target selective treatment. Therefore, the aim of the present study was to compare the sensitivity and specificity of the FAMACHA© method in the detection of Haemonchus contortus anemia in goats and sheep. For this, goats and sheep were evaluated from Embrapa Caprinos e Ovinos and from EAJ/UFRN, composed by 264 crossbred sheep and 256 crossbred goats of both sexes, aged between 3 and 5 months, respectively, kept on pasture native to Caatinga and Tanzania grass for a period between 98 and 225 days, totaling 4568 observations for sheep and 825 for goats. Four standard values of packed cell volume (PCV) ≤15%, ≤18%, ≤19%, ≤22%, were used to confirm anemia. Specificity (Sp) and sensitivity (Se) were determined. The correlation between FAMACHA©, PCV and egg counts per gram of faeces (OPG) was calculated using the Spearman correlation coefficient. To evaluate the concordance between FAMACHA© and PCV, the Kappa value (Ƙ) was obtained. At all PCV cut-off points, the FAMACHA© Se in Embrapa Caprinos e Ovinos sheep increased when FAMACHA© 3 animals were included as anemic (75% to 92%). Sp was higher in goats than in sheep at all PCV cut-off points when FAMACHA© 3 animals were not included as anemic (99.4% and 99.8%). For the EAJ/UFRN sheep herd, higher values of Se were observed when FAMACHA© 3 animals were included as anemic (48.5% to 51%). The Sp of these animals ranged from 88.1% to 90.1% when FAMACHA© 3 animals were not included as anemic. The evaluation of the FAMACHA© method for the goats of the EAJ/UFRN was not significant, since the parasite load of H. contortus did not reach the recommended minimum of 60%. The highest values of correct treatments were found at all PCV cut-off points for both Embrapa Capinos e Ovinos herds when FAMACHA© 4 and 5 were considered positive for anemia (75.7% to 91% for sheep and 82.1% to 98.5% for goats). In the sheep herd of EAJ/UFRN the highest values of correct treatment were found when FAMACHA© 3 animals were not included as anemic (65.13% to 78.2%). The value for Embrapa Caprinos e Ovinos sheep herd ranged from 0.320 < Ƙ < 0.560, higher when FAMACHA© 3 was not included as positive for anemia, indicating a reasonable to moderate agreement. For goats, the value Ƙ = 0.620 for the PCV ≤19 was observed, with substantial agreement being obtained. EAJ/UFRN herd, values indicated a reasonable concordance for sheep (0.067 < Κ < 0.074) and poor agreement for goats (Ƙ ≤ 0.02, p> 0.05). These results corroborate the applicability of the FAMACHA© method in cases of haemocoses in both herds. The PCV ≤15 cutoff point proved to be quite adequate for both species because it has high sensitivity indexes, but studies aiming at a better standardization of PCV cut values in goats are still necessary to increase the Se of the method for this species.

2
  • PEDRO HENRIQUE FERREIRA CAVALCANTI
  • Genetic Characterization and Phylogeny of HIV-1 in Rio Grande do Norte

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • GONZALO JOSE BELLO BENTANCOR
  • JOAO FELIPE BEZERRA
  • JOSE VERISSIMO FERNANDES
  • JOSELIO MARIA GALVAO DE ARAUJO
  • Data: 28 févr. 2019


  • Afficher le Résumé
  • From the earliest reports of AIDS in the 1980s to the present day, HIV has claimed approximately 35 million lives and is considered one of the worst pandemics ever recorded in humans. HIV is an RNA virus belonging to the family Retroviridae, subfamily Orthoretrovirinae of the genus Lentivirus. Although there are two species of HIV, only HIV-1 has a pandemic distribution. Phylogenetically the virus is divided into several groups, subtypes, sub-types, CRF's and URF's. With the advent of highly active antiretroviral therapy (HAART) HIV infection is considered to be chronic, the well treated individual will not develop AIDS, leading to an improvement in the quality of life and the possibility of non-transmissibility. However, increasing antiretroviral resistance, mainly due to poor adherence to treatment, could hamper the global progress made so far in combating HIV. The objective of this study was to perform the phylogenetic characterization of circulating HIV-1 strains in the State of Rio Grande do Norte from samples from January to October 2018. A total of 170 samples from the four health regions of Rio Grande do Norte were selected, meeting the established inclusion criteria, and in 8 samples it was possible to perform all the methodological steps of the research. The phylogeny was carried out by means of sequencing of the protease and reverse transcriptase regions of the Pol gene, indicating predominance of circulating HIV-1-B with one patient presenting the F1 subtype and one presenting subtype C. The analysis of the genetic sequencing of the viruses indicated the presence of several mutations of resistance, both transmitted and acquired, as well as accessory mutations, only mutations of clinical importance were not detected in 4 samples, the most recurrent mutations were modeled and identified in reverse transcriptase in regions of α-helix, β-sheet and region of shoulder strap. In conclusion, this study revealed a predominance of circulating HIV-1 subtype B and the mutation profile revealed that the most impaired antiretrovirals were reverse transcriptase inhibitors. The information is fundamental for adequate redirection of therapeutic rescue of these patients and molecular epidemiological screening of circulating strains.

3
  • SARA ESTER DE LIMA COSTA
  • Antiproliferative and cytotoxic activity of essential oil and hydroalcoholic extract from Lippia grata Schauer.

  • Leader : VANIA SOUSA ANDRADE
  • MEMBRES DE LA BANQUE :
  • VANIA SOUSA ANDRADE
  • JOSE VERISSIMO FERNANDES
  • LAURA ANDRADE CUSTODIO DE OLIVEIRA
  • Data: 8 mars 2019


  • Afficher le Résumé
  • The Caatinga is rich in a flora well adapted to its predominant climate, the semi-arid. Some plants characteristic of this climate has already been studied due to their medicinal properties. Within this context, we highlight the Lippia gracilis, known as alecrim-da-chapada of the Verbenaceae family, usually used for the treatment of throat and stomach disorders, through its oils and extracts. The objective of the present study was to evaluate the cytotoxicity and anti-proliferative activity of the use of the essential oil and hydroalcoholic extract of L. gracilis in the gastric adenocarcinoma cell line (AGS) in celula. For this, cell viability evaluation was performed by means of MTT reduction, clonogenic assay and flow cytometry, as well as the analysis of the chemical components present in the essential oil through gas chromatography associated with mass spectrometry (GC/MS). The major components of the phytochemical analysis of the essential oil were carvacrol (51.44%) followed by thymol (15.61%), monoterpenes that proved to have antioxidant, anti-inflammatory and antimicrobial action. Cell viability was assessed at 24 h, 48 h and 72 h at concentrations of 125, 250, 500, 750 and 1000 μg/mL for reduction of MTT in both AGS and normal murine fibroblast (3T3) cell lines. Similar cell death for AGS was observed in 48 h and 72 h and different in 24 h, both in oil and extract; 3T3 presented cell death only at the concentration of 1000 μg/mL in the period of 24 h, whereas in the periods of 48 h and 72 h the percentage of death was similar between periods from the concentration of 500 μg/mL. The clonogenic assay (1 x 102 cells/well) was given over a period of 10 days at concentrations of 125 to 500 μg/mL, showing results of 100% reduction of tumor cell proliferation. The flow cytometry was performed using the 48 h period and 500 μg/mL concentration, revealing 0.12% cell viability using the extract and 1.09% using the oil, whereas the negative control presented 98%. These results reveal a high anti-proliferative potential when compared to the control, induction of apoptosis and possible anticarcinogenic activity of the oil and extract of L. gracilis, besides antioxidant and cytotoxic activity. In view of this, plant extracts and essential oils of L. gracilis showed an important anticarcinogenic effect, suggesting new experimental stages for validation of these compounds in the alternative therapy to the affections of gastric tissue, if they are validated as phytopharmaceuticals.

4
  • GIANCARLO PAIVA NICOLETTI
  • PREVALENCE AND FACTORS ASSOCIATED WITH Gardnerella vaginalis IN WOMEN ATTENDED IN A GYNECOLOGICAL CLINIC IN THE MUNICIPALITY OF NATAL-RN

  • Leader : MARIA CELESTE NUNES DE MELO
  • MEMBRES DE LA BANQUE :
  • MARIA CELESTE NUNES DE MELO
  • JOSE VERISSIMO FERNANDES
  • DALIANA CALDAS PESSOA DA SILVA
  • Data: 15 mars 2019


  • Afficher le Résumé
  • The bacterial species Gardnerella vaginalis is acquired sexually and can colonize in the female genital tract, and is closely related to the bacterial vaginosis. Therefore, the presence of this species represents a risk factor for women during the gestation period and STI acquisition. The objective of this research was to evaluate the prevalence of Gardnerella vaginalis and associated factors in women attending a gynecological clinic in the city of Natal-RN. It is a cross-sectional descriptive study, whose target population was composed of 395 patients consulted at a private gynecological clinic in the city of Natal-RN. The study was preformed with the cytological analysis of the vaginal discharge according to the Papanicolau method and afterwards, the criteria (presence of discharge, amine test, coilocytes and clue cells) were evaluated to confirm the suggestive microorganisms. Sociodemographic information of sexual behavior and clinical conditions of the participants were collected from the medical records and a 10-questions personal interview. The crude and adjusted Prevalence Ratios (PR) were calculated (Prevalence Ratios of Mantel Haenszel, according to the type of access to the health system). Pearson's Chi-square test (x2) was used to evaluate the presence of Gardnerella vaginalis and patients` associated factors. The overall prevalence of Gardnerella vaginalis was 35.4%, 51% higher in the age group of 15-44 years (p-value = 0.004), 45% higher in married women (p-value = 0.036), and 36% lower among women taking contraceptives (p-value = 0.004). The cases of G. vaginalis were 3.2 times higher among women who had a suggestive HPV diagnosis (p-value <0.001). The prevalence of G.vaginalis was 26 times greater in the group of women with vaginal discharge and 15 times greater in the group of women with a positive amine test (p-value <0.001). In this perspective, the interaction of positive amine and vaginal discharge was tested, it was observed that there was a statistically significant association with the presence of G. vaginalis (PR = 14.0; p-value <0.001). A significant prevalence of G.vaginalis colonizing the patients' vagina was found, in addition to the statistically significant association between the presence of this bacterium and some behavioral and sexual factors. The socioeconomic difference was not relevant in the study, so it is important to analyze the clinical and behavioral criteria for the diagnosis of Bacterial Vaginosis.

5
  • MARÍLIA FARIAS DE MELO
  • PROFILE OF CYTOKINES IN PATIENTS DURING THE ACUTE PHASE OF INFECTION BY THE CHIKUNGUNYA VIRUS IN RIO GRANDE DO NORTE

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • JOSELIO MARIA GALVAO DE ARAUJO
  • JANEUSA TRINDADE DE SOUTO
  • NATHALIE DE SENA PEREIRA
  • Data: 15 mars 2019


  • Afficher le Résumé
  • Chikungunya fever is arbovirus transmitted by arthropods of the genus Aedes, which causes a great economic impact and implications for the world public health. The Chikungunya virus (CHIKV) belongs to the Togaviridae family and to the genus Alphavirus, characterized as simple polarized positive-stranded RNA viruses, enveloped and spherical. In this study, the profile of cytokines characteristic of the innate and adaptive immune response presented by patients with acute CHIKV infection was evaluated by quantifying the expression of Tbet, GATA3, PU.1, RORγT, AHR and FoxP3 transcription factors and of the gene expression of the cytokines IFN-γ, IL-4, IL-9, IL-17, IL-22, IL-10 and TGFβ by qRT-PCR. Blood samples from 27 patients with previous diagnosis of CHIKV in the acute phase and nine blood samples from healthy individuals, used as control group, were analyzed. The acute phase CHIKV-infected patients presented high and significant levels for the expression of Tbet, GATA3, RORγT and FoxP3 transcription factors, as well as the expression of cytokines: IFN-γ, IL-4, IL-17 and IL- 10. Thus, infected patients had a predominantly proinflammatory immune response profile, with the expression of transcription factors and cytokines that may be derived from innate immune response cells, innate lymphoid cells (ILCs), and Th1 cell subpopulations , Th2, Th17 and Treg.

     

    A Febre Chikungunya é uma arbovirose transmitida por artrópodes do gênero Aedes, que causam um grande impacto econômico e implicações para a saúde pública mundial. O vírus Chikungunya (CHIKV) pertence à família Togaviridae e ao gênero Alphavirus, caracterizados como vírus de RNA de fita simples polaridade positiva, envelopados e esféricos. Neste estu  do, foi avaliado o perfil de citocinas características da resposta imune inata e adaptativa apresentado por pacientes com infecção aguda pelo CHIKV, por meio da quantificação da expressão de fatores de transcrição Tbet, GATA3, PU.1, RORγT, AHR e FoxP3 e da expressão gênica das citocinas IFN-γ, IL-4, IL-9, IL-17, IL-22, IL-10 e TGFβ, por meio de qRT-PCR. Foram analisadas amostras de sangue de 27 pacientes com diagnóstico prévio de CHIKV na fase aguda e nove amostras de sangue de indivíduos saudáveis, utilizados como grupo controle. Os pacientes em fase aguda infectados pelo CHIKV apresentaram níveis elevados e significativos para a expressão dos fatores de transcrição Tbet, GATA3, RORγT e FoxP3, como também para a expressão das citocinas: IFN-γ, IL-4, IL-17 e IL-10. Desde modo, os pacientes infectados apresentaram um perfil de resposta imune majoritariamente pró-inflamatória, com a expressão de fatores de transcrição e citocinas que podem ser provenientes de células da resposta imune inata, as células linfóides inatas (ILCs) e das subpopulações de células Th1, Th2, Th17 e Treg.

     

6
  • RAMAYANA MORAIS DE MEDEIROS BRITO
  • EVALUATION OF THE EFFECTS OF TOXOPLASMA GONDII INFECTION ON THE BRAIN TISSUE STRUCTURE AND INFLUENCE ON THE NEUROIMOPATHOGENIC RESPONSE

  • Leader : VALTER FERREIRA DE ANDRADE NETO
  • MEMBRES DE LA BANQUE :
  • VALTER FERREIRA DE ANDRADE NETO
  • PAULO MARCOS DA MATTA GUEDES
  • ANDRE TALVANI PEDROSA DA SILVA
  • Data: 25 avr. 2019


  • Afficher le Résumé
  • Toxoplasmosis is a zoonosis widely distributed throughout the world, caused by Toxoplasma gondii, an obligate intracellular protozoan capable of invading and multiplying in any nucleated cell. After invasion, the parasite triggers the activation of a pro-inflammatory response, capable of stimulating its encistamento and maintenance of the chronic infection. This antiparasitic response, when exacerbated, can generate immunopathological changes in the host organism. However, in order to avoid tissue compromise, the immune system establishes a balance through the anti-inflammatory response. However, the parasite has mechanisms to manipulate the immune system in order to establish a persistent and efficient infection, contributing to the development of brain changes as a consequence of chronic neuroinflammation. In this context, the objective of this work was to evaluate the effects of T. gondii cystogenic strain on the inflammatory response in isogenic mice (Balb / ce C57BL / 6) and outbred (Swiss), related to neuropathological features characteristic of the infection cerebral. Among the infected groups, all lineages presented body weight loss and evident signs of acute infection, with reactivity index (IR) for anti-T IgG. gondii, characterizing the positivity of the infection. The quantification of proinflammatory cytokines showed that the production of IFN-γ, IL-12 and TNF-α by the infected groups of the three strains was higher than those presented by the control groups, reinforcing the importance of a pro- effective in the process of chronic infection. In contrast, for anti-inflammatory cytokines, TGF-β production was more pronounced only by the Balb / ce infected group, for IL-10, the three lines showed similar and high values, indicating the immunoregulation involvement in the balance of developed pathogenesis. These data reinforce the importance of the development of an efficient immune response in the maintenance of the cysts found in the brain, characteristic of the chronic infection, varying according to the profile presented by the different strains of mice.

2018
Thèses
1
  • LEANDRO GURGEL DE MEDEIROS
  • .

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • JOSELIO MARIA GALVAO DE ARAUJO
  • JOSE VERISSIMO FERNANDES
  • RENATA ANTONACI GAMA
  • FABÍOLA DA CRUZ NUNES
  • Data: 19 févr. 2018


  • Afficher le Résumé
  • .

2
  • DENIS DANTAS DA SILVA
  • QUANTIFICATION OF ANTI M2-PIRUVATO KINASE AUTOANTIBODIES IN PATIENTS WITH DIFFERENT CLINICAL FORMS OF CHAGAS DISEASE

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • PAULO MARCOS DA MATTA GUEDES
  • RENATA ANTONACI GAMA
  • MANUELA SALES LIMA NASCIMENTO
  • Data: 27 févr. 2018


  • Afficher le Résumé
  • Digestive and cardiodigestive forms of Chagas' disease are observed in 2% to 15% of patients and immunopathological mechanisms that induce its development remain undefined. In addition, the determination of esophagus and colon involvement in patients is due to invasive and uncomfortable tests, which in most cases are not performed, allowing the patient to evolve to a more severe form of the pathology with a worse prognosis. In this work we evaluated the involvement of the production of anti M2-pyruvate kinase autoantibodies (M2-PK) and its possible association with the development and / or diagnosis of the digestive form of Chagas' disease. The production of total IgG and isotypes (IgG1, IgG2, IgG3, IgG4) was quantified using Trypanosoma cruzi epimastigote forms antigen and human M2-PK protein by serum ELISA in patients with indeterminate forms (n = 30), cardiac (n = 30), digestive (n = 15) and cardiodigestive (n = 15) of Chagas' disease, and correlated with the degree of dilation of the esophagus and colon. Serum samples from uninfected patients (n = 30) were used as controls. Chagasic patients with indeterminate, cardiac, digestive and cardiodigestive clinical forms had higher total antiT-cruzi IgG antibody and auto M2-PK autoantibodies when compared to uninfected individuals. Patients with the digestive and cardiodigestive form of the disease had higher production of total IgG autoantibodies compared to those with an indeterminate form. These patients still had a higher production of anti M2-PK autoantibodies belonging to the IgG1 and IgG4 isotypes when compared to patients with the indeterminate and cardiac forms. There was no difference between the production of the IgG2 and IgG3 isotypes in the patients presenting the different clinical manifestations using T. cruzi antigen or the M2-PK protein. The results indicate that the increase in the production of anti M2-PK autoantibodies may be related to the development of the digestive form of Chagas' disease.

3
  • HANNALY WANA BEZERRA PEREIRA
  • .

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • JOSELIO MARIA GALVAO DE ARAUJO
  • JOSE VERISSIMO FERNANDES
  • THALES ALLYRIO ARAUJO DE MEDEIROS FERNANDES
  • Data: 27 févr. 2018


  • Afficher le Résumé
  • .

4
  • BRENDA ELEN BIZERRA ALVES
  • .

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • DANIEL CARLOS FERREIRA LANZA
  • JOAO FELIPE BEZERRA
  • JOSE VERISSIMO FERNANDES
  • JOSELIO MARIA GALVAO DE ARAUJO
  • Data: 28 févr. 2018


  • Afficher le Résumé
  • .

5
  • MARCELO HENRIQUE MATIAS DA SILVA
  • Profile of immune innate response of patients infected with Zika virus

  • Leader : JOSE VERISSIMO FERNANDES
  • MEMBRES DE LA BANQUE :
  • JOSE VERISSIMO FERNANDES
  • JOSELIO MARIA GALVAO DE ARAUJO
  • MANUELA SALES LIMA NASCIMENTO
  • Data: 2 mars 2018


  • Afficher le Résumé
  • -

6
  • JULLIETTE MEDEIROS DE OLIVEIRA GALVAO
  • -

  • Leader : MARIA CELESTE NUNES DE MELO
  • MEMBRES DE LA BANQUE :
  • MARIA CELESTE NUNES DE MELO
  • VANIA SOUSA ANDRADE
  • ANA ISABELA LOPES SALES MOIOLI
  • Data: 2 mars 2018


  • Afficher le Résumé
  • -

7
  • MÁRJORE LORENA DE MELO SILVA
  • .

  • Leader : RENATA ANTONACI GAMA
  • MEMBRES DE LA BANQUE :
  • RENATA ANTONACI GAMA
  • PAULO MARCOS DA MATTA GUEDES
  • JOSE HERIBERTO OLIVEIRA DO NASCIMENTO
  • PATRICIA BATISTA BARRA MEDEIROS BARBOSA
  • Data: 26 mars 2018


  • Afficher le Résumé
  • .

8
  • CAMILA MARTINS GOMES MORAIS
  • -

  • Leader : VALTER FERREIRA DE ANDRADE NETO
  • MEMBRES DE LA BANQUE :
  • CARLOS CAPISTRANO GONCALVES DE OLIVEIRA
  • DANIEL DE LIMA PONTES
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • VALTER FERREIRA DE ANDRADE NETO
  • Data: 5 avr. 2018


  • Afficher le Résumé
  • -

2017
Thèses
1
  • LAISE DIANA DOS SANTOS BRANDAO
  • -

  • Leader : VANIA SOUSA ANDRADE
  • MEMBRES DE LA BANQUE :
  • MARIA APARECIDA RESENDE STOIANOFF
  • MARIA CELESTE NUNES DE MELO
  • VANIA SOUSA ANDRADE
  • Data: 27 janv. 2017


  • Afficher le Résumé
  • -

2
  • ÍTALO DIEGO REBOUÇAS DE ARAÚJO
  • .

  • Leader : VANIA SOUSA ANDRADE
  • MEMBRES DE LA BANQUE :
  • LYSETE MARIA DE ASSIS BASTOS
  • VANESSA DE PAULA SOARES RACHETTI
  • VANIA SOUSA ANDRADE
  • Data: 30 janv. 2017


  • Afficher le Résumé
  • Nowadays, studies in the field of medicinal chemistry have been intensifying in order to elucidate new phytopharmaceuticals, either by obtaining extracts, fractions, isolated compounds or essential oils that present some type of biological activity. In this context, the aroeira-do-sertão (Myracrodruon urundeuva), of the family Anacardiaceae, already studied regarding the antimicrobial, anti-inflammatory and cicatrizant potential stands out. Motivated by new therapeutic alternatives, considering the growing microbial resistance, this study evaluated the antimicrobial activity of natural products obtained from the leaves of said plant. Among these is an essential oil, which was extracted by hydrodistillation, characterized by NMR and GC-MS, and evaluated for cytotoxicity; In addition, organic extracts, which were only analyzed for antimicrobial activity: lyophilized methanolic obtained by decoction; Chloroform and ethyl acetate, extracted at room temperature with their respective solvents and filtered under reduced pressure. The antibacterial activity was evaluated by the microdilution technique in broth, in which the MICs were determined using CTT (2,3,5-triphenyl-tetrazolium chloride) as a bacterial growth promoter, and the CBMs were analyzed by growth analysis of the contents of wells on BHI agar. The cytotoxicity of the oil was evaluated by the MTT method, 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide. The oil, in the chemical characterization, among the terpenes identified, had as main constituent the α-pinene (87,85%). In addition, this oil showed antibacterial activity against all strains tested, where for some of these, equivalence between MIC and MBC values, which were 0,22 mg/ml for Staphylococcus aureus, 0,44 mg/ml for Salmonella Enteritidis and 7 mg/ml for Pseudomonas aeruginosa. For Staphylococcus epidermidis the MIC was 0,11 mg/ml and the MBC was 0,22 mg/ml. Escherichia coli was inhibited with MIC of 0,88 mg/ml and MBC of 1,75 mg/ml. Equivalence between MIC and MBC was observed for methanolic extract against S. epidermidis (9,75 mg/ml). For S. aureus, the MIC of this extract was 9,75 mg/ml and the MBC 78 mg/ml. They were resistant to such extract: E. coli, S. Enteritidis and P. aeruginosa. Chloroform and ethyl acetate extracts were bacteriostatic against the five strains, but chloroform inhibited them all with MICs of 15 mg/ml, while ethyl acetate had MICs of 7,56 mg/ml for S. aureus, 1,89 mg/ml for S. epidermidis, 15,12 mg/ml for S. Enteritidis and 30,25 mg/ml for E. coli and P. aeruginosa. As for cytotoxicity, the essential oil compromised the cell viability of the Vero E6 line, only at the highest concentration, 4,4 mg/mL, inhibiting about 93,91% in 24h and 94,26% in 48h. In HeLa cells, in 24h the oil at the same dose had inhibition of 21%, which after 48h increased to 44,3%, showing a possible antitumor action. For the non-tumor cell line HEK-293, the oil had no toxic effect on them. It is concluded that the results are promising, opening future prospects for M. urundeuva leaf products to be pharmacologically viable.

3
  • MARIA DA CONCEIÇÃO ALEXANDRE CASTRO
  • Evaluation of the occurrence of species Candida parapsilosis complex in biological samples of patients and professionals of hospitals Natal/RN.

  • Leader : RAQUEL CORDEIRO THEODORO
  • MEMBRES DE LA BANQUE :
  • EVELINE PIPOLO MILAN
  • RAQUEL CORDEIRO THEODORO
  • REGINALDO GONÇALVES DE LIMA NETO
  • Data: 22 févr. 2017


  • Afficher le Résumé
  • Among Candida non-albicans species, the Candida parapsilosis complex (C. parapsilosis stricto sensu, C. orthopsilosis and C. metapsilosis) is the most frequente in systemic infections, being found in hands of health professionals, biomaterials and infusions as the main sources of nosocomial infection. Since these species have different virulence and antifungal susceptibility profiles, the species-specific diagnosis may contribute for the most suitable therapeutic choice. The aim of this research was to compare the occurrence of Candida parapsilosis species and the other Candida species in clinical samples isolated by LACEN-RN and Centro de Patologia Clínica de Natal/RN and in samples obtained in hands and buccal mucosa of health professionals from the ICU of Giselda Trigueiro and Monsenhor Walfredo Gurgel hospitals, from august 2015 to September 2015. The species identification was previously performed by Vitek equipment and alternatively by Chromagar, ITS1-5.8S-ITS2 sequencing or PCR of the VMA intein (for distinguishing among the species from C. parapsilosis complex). Among the 163 clinical isolates, C. albicans (54%), C. tropicalis (18%), C. parapsilosis complex (14%) and C. glabrata (7%) were the most prevalent, whereas in the 196 isolated collected from health professionals the most prevalent Candida species were C. albicans (4%), C. parapsilosis complex (4%) and C. tropicalis (1%). Our research shows that C. albicans is still the most prevalent species, but the increase in C. tropicalis infections and C. parapsilosis complex is notorious and worrying. The evaluation of the antifungal susceptibility profile of the different species of the C. parapsilosiscomplex studied here did not show marked differences in antifungal activity. Our data are similar to other researches carried out in Brazil, which report the C. parapsilosis complex as the second or third most frequent among Candida non-albicans in hospital infections, being this the first study on the occurrence of C. parapsilosis complex in the state of Rio Grande do Norte/RN using the VMA intein for species identification. 

4
  • MEIRE KARLA MIGUEL CRUZ SANTOS
  • Innate immune receptors in canine visceral leishmaniasis

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • PAULO MARCOS DA MATTA GUEDES
  • VALTER FERREIRA DE ANDRADE NETO
  • MARIA ADELAIDE DO VALLE MATTA
  • Data: 23 févr. 2017


  • Afficher le Résumé
  • Dogs are the primary reservoirs of parasites of the Leishmania genus. Innate immune receptors perform early detection of the parasite and lead to specific adaptive immune response in attempt to infection control. However, few studies have investigated a correlation between the expression of innate immunity receptors and the resistance or susceptibility pattern in dogs naturally infected with Leishmania infantum. The aim of this study was to correlate the clinical status of dogs naturally infected with L. infantum with the mRNA expression levels of innate imune receptors (Toll like receptors-TLRs and Nod Like Receptors-NLRs). Initially, serum of 76 dogs was collected at the Zoonoses Control Center in Natal, Rio Grande do Norte, Brazil. The L. infantum infection in dogs was confirmed by ELISA and DPP® tests. Subsequently, animals were clinially evaluated and classified as asymptomatic (n=19), oligosymptomatic (n=19), symptomatic (n=19) and uninfected (n=19). Dogs naturally infected by L. infantum and uninfected controls were euthanasied and liver samples were collected to quantify mRNA expression of TLRs (TLR1-9), Nod Like receptors-NLRs (NOD1, NOD2, NLRP1, NLRP3), cytokines (IL1β, IL-6, IL-12, IL-10, TNFα, IFN-γ) and iNOS using real-time PCR. The results demonstrate the increased expression of almost all TLRs and NLRs in dogs naturally infected by L. infantum compared with uninfected animals. However, symptomatic dogs showed higher expression of TLR1, TLR2, TLR3, TLR4, TLR5, TLR7, TLR8 NLRP1, NLRP3, NOD1 and IL-1β than asymptomatic animals, revealing significant up regulation of transcription with disease progression. On the other hand, asymptomatic dogs presented greater cytokine mRNA expression (IFN-γ, IL-12) and iNOS when compared to oligosymptomatic and asymptomatic animals. This study unveil new knowledge involving innate immunity receptors (TLRs, NLRs, NLRPs) and cytokines in canine visceral leishmaniasis and may be used as a basis for better understanding of resistance or susceptibility mechanisms in dogs infected with L. infantum, as well as prophylactic strategies to control canine visceral leishmaniasis.

5
  • POLYANNA SILVA MOREIRA
  • .

  • Leader : PAULA RENATA LIMA MACHADO
  • MEMBRES DE LA BANQUE :
  • NALU TEIXEIRA DE AGUIAR PERES
  • PAULA RENATA LIMA MACHADO
  • VANIA SOUSA ANDRADE
  • Data: 23 févr. 2017


  • Afficher le Résumé
  • .

6
  • NAYANA LUIZA SOARES DE ARAUJO GARCIA
  • Chemokine expression in patients with different clinical forms of Chagas disease

     

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • MANUELA SALES LIMA NASCIMENTO
  • PAULO MARCOS DA MATTA GUEDES
  • RENATA ANTONACI GAMA
  • Data: 24 févr. 2017


  • Afficher le Résumé
  • Chemokines act in the recruitment and accumulation of leukocytes during the inflammatory process and plays an important role in the development of the different clinical forms of Chagas’ disease. The aim of this study was to evaluate the mRNA expression of chemokines (CCL1, CCL2, CCL3, CCL4, CCL5, CCL17, CCL22, CCL24, CCL27, CCL28, CXCL9, CXCL10) and chemokine receptors (CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR10, CXCR3) in chronic chagasic patients with indeterminate (n=18), cardiac (n=17), digestive (n=15) and cardiodigestive (n=15) clinical forms. The mRNA expression was performed in peripheral-blood mononuclear cells by real-time PCR. Patients with the cardiac form displayed higher mRNA expression of CXCL9, CXCL10, CXCR3 and CCR5, than patients with indeterminate form. On the other hand, patients with the digestive form showed high expression of CCR3, when compared to patients with indeterminate and cardiac clinical forms of the disease. In addition there was possible a positively correlation beteween CCR3 mRNA expression and sigmoid dimension. The chemokine CCL5 had higher mRNA expression in cardiodigestive patients compared to those with the cardiac and indeterminate forms. The chemokines CCL1, CCL2, CCL3, CCL4, CCL17, CCL22, CCL24, CCL27, CCL28, and the chemokine receptors CCR2, CCR4, CCR6, CCR7, CCR8 and CCR10 did not show significant differences in mRNA expression among the patients with the different clinical forms. CXCL9, CXCL10, CXCR3 and CCR5 participate in the migration of Th1-profile cells and the high expression in patients with chagasic cardiomyopathy indicates their contribution in the cardiac inflammatory process. Patients with digestive form showed high of CCR3 mRNA expression which is involved with Th2 immune profile, indicating possible polarization for this profile in development of digestive form of disease.

7
  • JOÃO CIRO FAGUNDES NETO
  • .

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • HECTOR GABRIEL ROMERO BRUNETTO
  • JOSELIO MARIA GALVAO DE ARAUJO
  • LEONARDO CAPISTRANO FERREIRA
  • RENATA ANTONACI GAMA
  • Data: 24 févr. 2017


  • Afficher le Résumé
  • .

8
  • KERCIA MONALINE DE SOUZA JOVENTINO
  • .

  • Leader : PAULA RENATA LIMA MACHADO
  • MEMBRES DE LA BANQUE :
  • IGOR THIAGO BORGES DE QUEIROZ E SILVA
  • PAULA RENATA LIMA MACHADO
  • VALERIA SORAYA DE FARIAS SALES
  • Data: 20 mars 2017


  • Afficher le Résumé
  • .

9
  • CAMILA CRISTINA GUIMARÃES NOBRE
  • .

  • Leader : JOSE VERISSIMO FERNANDES
  • MEMBRES DE LA BANQUE :
  • JOSE VERISSIMO FERNANDES
  • JOSELIO MARIA GALVAO DE ARAUJO
  • RICARDO NEY OLIVEIRA COBUCCI
  • THALES ALLYRIO ARAUJO DE MEDEIROS FERNANDES
  • Data: 31 mai 2017


  • Afficher le Résumé
  • .

2016
Thèses
1
  • THIAGO ANDRÉ CIDRAL
  • Resistência à linezolida em Estafilococos Coagulase Negativos resistentes à meticilina provenientes de hospitais da cidade do Natal-RN

  • Leader : MARIA CELESTE NUNES DE MELO
  • MEMBRES DE LA BANQUE :
  • MARIA CELESTE NUNES DE MELO
  • RAQUEL CORDEIRO THEODORO
  • THALES ALLYRIO ARAUJO DE MEDEIROS FERNANDES
  • Data: 15 janv. 2016


  • Afficher le Résumé
  • Os Estafilococos Coagulase Negativos (ECN) são microrganismos pertencentes à microbiota normal da pele e de mucosas dos seres humanos e de animais. A maioria das infecções causadas por ECN estão relacionadas ao uso de dispositivos médicos invasivos que ao lesionar a integridade da pele servem de base para a formação de biofilmes, um importante fator de virulência. Grande parte dos isolados de coagulase negativo são provenientes de hemoculturas e pontas de cateter e nos últimos anos vem se tornando um grave problema no que diz respeito à antibioticoterapia, em virtude do número elevado de cepas multirresistentes descritas. O objetivo deste trabalho foi pesquisar resistência à linezolida em estafilococos coagulase negativos resistentes à meticilina isolados de ponta de cateter e hemocultura de hospitais públicos e privados da cidade do Natal. Os isolados bacterianos foram coletados a partir de demanda espontânea em Hospitais Públicos e Privados. O gênero Staphylococcus foi confirmado através dos testes de rotina como coloração de Gram, prova da catalase da coagulase livre. A identificação a nível de espécie foi realizada através de testes bioquímicos convencionais. Algumas amostras tiveram sua identificação confirmada pelos sistemas VITEK 2 e MALDI-TOF. O perfil de resistência aos antimicrobianos foi avaliado através da técnica de disco-difusão (CLSI 2013). A Concentração Inibitória Mínima para vancomicina e linezolida foi determinada através do uso de E-test e a presença dos genes mecA e cfr foi confirmada pela técnica da Reação em Cadeia da Polimerase. Algumas amostras tiveram a região V da subunidade 23S do gene do rRNA sequenciadas e analisadas. Posteriormente, as mesmas foram submetidas a técnica do PFGE para determinação do seu pulsotipo. Dos 43 estafilococos coagulase negativos resistentes à oxacilina incluídos neste estudo, 33 (77%) foram identificados como S. epidermidis, 6 (14%) como S. haemolyticus, 3 (7%) como S. homins e 1 (2%) como S. capitis. Os isolados de hemocultura representaram 86% (37) e os de ponta de cateter 14% (6). As amostras apresentaram um perfil de multirresistência, uma vez que 42 dos 43 isolados apresentaram resistência à 4 ou mais classes de drogas. Todas apresentaram o gene mecA. Nenhuma amostra apresentou resistência à vancomicina. Três cepas de S. hominis e duas de S. epidermidis, apresentaram resistência à linezolida com CIM variando entre 6 e 64 µL/mL. Quando investigadas, apresentaram duas mutações pontuais (C2190T e G2603T) na região V do gene para rRNA 23S. Nenhuma destas apresentou o gene cfr. O PFGE dos S. hominis revelou a presença de um único pulsotipo em 3 hospitais, enquanto não foi encontrado semelhança genética entre os S. epidermidis. Estes achados destacam a importância da vigilância continuada em relação a resistência a linezolida no gênero Staphylococcus.

2
  • VALBER RUDNELLY MEIRA
  • Avaliação da resposta imune em ovinos após imunização com antígenos SAG1, SAG2 e SAG3 candidatos à vacina anti-Toxoplasmose.

  • Leader : VALTER FERREIRA DE ANDRADE NETO
  • MEMBRES DE LA BANQUE :
  • ANA CARLA DIÓGENES SUASSUNA BEZERRA
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • VALTER FERREIRA DE ANDRADE NETO
  • Data: 22 janv. 2016


  • Afficher le Résumé
  • A Toxoplasmose é uma zoonose cosmopolita causada pelo protozoário Toxoplasma gondii. Os ovinos, dentre os animais de produção, configuram-se como uma das espécies que apresentam uma maior susceptibilidade a este parasito, sendo um importante agente envolvido na ocorrência de abortos entre animais ruminantes, provocando perdas econômicas significantes e repercussões na saúde pública, uma vez que o consumo de produtos de origem ovina constituem-se como uma importante fonte de infecção para o homem. Neste estudo foram coletadas amostras de sangue de 90 ovinos em 02 fazendas situadas nos municípios de Macaíba e de Ceará Mirim, região metropolitana de Natal, Estado do Rio Grande do Norte. Os animais incluídos nesse estudo foram selecionados sorologicamente por ELISA e HAI e a resposta imune humoral dos ovinos imunizados com adenovírus recombinantes codificando SAGs foi avaliada por ELISA. A soroprevalência encontrada na Escola de Jundiaí foi de 50%, enquanto que na Fazenda Lanila foi de 25,6%.Provavelmente o manejo dos animais nessas fazendas está associado com a diferença de positividade observada.Os resultados preliminares mostram que nosso protocolo de imunização, avaliado 30 dias após a primeira dose, mostrou capacidade em estimular a produção de IgG total específicos contra os antígenos SAGs de T.gondii e que são importantes mecanismos de defesa principalmente do ponto de vista congênito e durante fase crônica da doença, especificamente AdSAG1, bem como não foram observadas alterações clínicas relevantes entre os animais vacinados. Estudos mais específicos são necessários para esclarecer de forma mais específica o impacto que tal regime de imunização exerce tanto na capacidade de proteção contra a parasitose e sobretudo se acarreta ou não algum dano a saúde do animal.

3
  • JOELMA DANTAS MONTEIRO
  • EPIDEMIOLOGIA MOLECULAR DOS VÍRUS DENGUE E ZIKA NO ESTADO DO RIO GRANDE DO NORTE, NO PERÍODO DE JUNHO DE 2014 A MAIO DE 2015.

  • Leader : JOSELIO MARIA GALVAO DE ARAUJO
  • MEMBRES DE LA BANQUE :
  • JOSE VERISSIMO FERNANDES
  • JOSELIO MARIA GALVAO DE ARAUJO
  • THALES ALLYRIO ARAUJO DE MEDEIROS FERNANDES
  • Data: 26 janv. 2016


  • Afficher le Résumé
  • A dengue e a febre da zika são doenças causadas por vírus RNA de fita simples, polaridade positiva, pertencentes à família Flaviviridae, gênero Flavivirus e são transmitidos ao homem através da picada de vetores artrópodes hematófagos do gênero Aedes. O Estado do Rio Grande do Norte (RN) convive com o aumento do número de casos da dengue há mais de duas décadas, porém, no que concerne à febre da zika, até os primeiros dias do mês de maio deste ano, o Ministério da Saúde não havia confirmado nenhum caso da doença no Brasil, tampouco no Rio Grande do Norte. Esse trabalho objetivou analisar o perfil epidemiológico dos vírus Dengue e Zika no Estado do Rio Grande do Norte, no período de junho de 2014 a maio de 2015. Foram estudadas 396 amostras provenientes de pacientes com casos suspeitos de dengue e/ou zika, das quais, 334 amostras foram analisadas para dengue através da RT-PCR, representando 8,08% (27/334) de positividade e 348 amostras foram estudadas para Zika por meio da qRT-PCR, onde o vírus foi confirmado em 20,98% (73/348) dos casos. Os sorotipos DENV-1, DENV-2 e DENV-4 cocircularam no RN, com predominância do último, detectado em 66,67% (18/27) dos casos positivos. Os municípios de Jandaíra, Natal e Ouro Branco foram os mais acometidos por dengue, com 18,52% (5/27), 22,22% (6/27) e 11,11% (3/27), respectivamente. No que se refere ao Zika, as cidades mais atingidas foram Guamaré, Natal, Nova Cruz e Parnamirim, com 13,70% (10/73), 30,14% (22/73), 9,59% (7/73) e 19,18% (14/73) de confirmações, respectivamente. Neste estudo, o primeiro município a ter um caso confirmado de dengue foi Jandaíra. Caiçara do Rio do Vento foi o primeiro a apresentar um caso de Zika, seguido de Galinhos. Junho foi o mês mais representativo para dengue com 44,44% (12/27) dos casos confirmados, enquanto Março foi o mais acometido por Zika com 23,29% (17/73). O gênero masculino e o feminino tiveram praticamente a mesma proporção de casos positivos para dengue, com 51,85% (14/27) e 48,15% (13/27), respectivamente, enquanto o Zika acometeu um maior número de mulheres, representando 57,53% (42/73). As faixas etárias mais acometidas por dengue foram a de 11-20 e 51-60 anos, cada uma com 18,52% (5/27). No que concerne ao vírus Zika, a faixa etária de 0-10 anos foi a mais acometida com 19,18% (14/73) dos casos confirmados, seguidas das faixas de 31-40 e 41-50 anos, onde cada uma destas representou 16,44% (12/73). Em decorrência da cocirculação desses flavivírus no RN, é fundamental compreender a prevalência e a dinâmica de circulação de ambos os vírus, no intuito de estabelecer medidas para controlar futuros surtos e epidemias no Estado. Este trabalho representa o maior estudo sobre o Zika vírus no Estado do RN.

4
  • MARIA EDUARDA DE SOUZA MENEZES DA COSTA
  • Novos alvos moleculares para detecção e genotipagem de Toxoplasma gondii

  • Leader : DANIEL CARLOS FERREIRA LANZA
  • MEMBRES DE LA BANQUE :
  • DANIEL CARLOS FERREIRA LANZA
  • PAULO MARCOS DA MATTA GUEDES
  • PAULA VIVIANE DE SOUZA QUEIROZ MOREIRA
  • Data: 27 janv. 2016


  • Afficher le Résumé
  • O Toxoplasma gondii é um parasito mundialmente distribuído, que pode causar desde sintomas parecidos com a gripe até problemas neurológicos. As cepas do T. gondii apresentam grande variabilidade genética na América do Sul, sendo fundamental a análise de sequências para auxiliar no diagnóstico confiável e para classificação dos diferentes genótipos. O objetivo deste trabalho foi desenvolver dois métodos moleculares, um para detecção e o outro para genotipagem do T. gondii, possibilitando a identificação de todas as cepas conhecidas e a geração de dados que possam ser correlacionados com a virulência. Inicialmente, foi utilizado o programa MUSCLE para alinhamento de 685 sequencias nucleotídicas obtidas do GenBank, em seguida os alinhamentos foram analisados no programa MEGA 6.0 para determinação de sua variabilidade genética. O gene GRA7 foi selecionado como alvo para os iniciadores, que foram desenhados em regiões conservadas do gene utilizando os programas Geneious 9.0 e Primer BLAST. O protocolo de amplificação utilizando-se os primers para o gene GRA7 foi então comparado com outros protocolos padronizados para amplificação do gene B1 e do elemento repetitivo de 529 pb, que são os marcadores mais utilizados para o diagnóstico do T. gondii. Para o desenvolvimento do sistema de genotipagem foram selecionados os genes ROP5, ROP18 e GRA7, que estão relacionados ao mecanismo de virulência melhor descrito em T. gondii. O sistema de genotipagem desenvolvido se baseia na análise de polimorfismos presentes em fragmentos sob seleção positiva desses genes, que permite identificar cepas pertencentes as linhagens clonais e cepas atípicas. Utilizando-se essa nova abordagem para a seleção de marcadores, será necessário investigar um número de regiões do genoma consideravelmente menor que o utilizado pelo método utilizado tradicionalmente, simplificando o processo. Concluindo, o desenho de primers em regiões conservadas do gene GRA7 permitiu o desenvolvimento de um sistema de detecção utilizando PCR com excelente positividade e sensibilidade, principalmente para detecção de cepas atípicas do T. gondii. Ainda, a genotipagem baseada na detecção de polimorfismos em genes de virulência permitiu a diferenciação genotípica das diferentes cepas de forma mais simples que a técnica de RFLP utilizada atualmente.

     

5
  • RENATO CÉSAR DE MELO FREIRE
  • STUDY OF CULICIDIAN FAUNA AND CIRCULATING ARBOVIRUSES IN THE FLONA AÇU-RN CONSERVATION UNIT

  • Leader : RENATA ANTONACI GAMA
  • MEMBRES DE LA BANQUE :
  • RENATA ANTONACI GAMA
  • JOSELIO MARIA GALVAO DE ARAUJO
  • ROSELI LA CORTE DOS SANTOS
  • Data: 28 janv. 2016


  • Afficher le Résumé
  • Culicids are fundamental parts in the transmission of several pathogens which makes this family of dipterans of great medical importance. One branch for understanding how these diseases can be controlled is the study of the vectors that transmit them. Therefore, the importance of carrying out studies that deal with the investigation of these vectors in areas that have not yet been explored, such as conservation units, that can house these pathogens. The objective of this work was to carry out a survey of the species of culicidae in the Caatinga and the possible presence of circulating arboviruses. Methodology: Mosquito collections were carried out from September 2011 to August 2013 in the National Forest of Açu-RN. Three types of traps were used: Ovitrampa, Larvitrampa and Shannon Trap, in order to collect the forms of egg, larva and adults respectively. The individuals collected were individually identified using specific morphological keys and the adults were submitted to viral identification, through the extraction of viral RNA, RT-PCR and sequencing of viruses. Results and Discussion: Eight genera of the Culicidae family were collected: Aedes, Aedeomyia, Anopheles, Coquillettidia, Culex, Haemagogus, Mansonia and Ochlerotatus. The Ovitrampa and Larvitrampa traps captured A aegypti, A albopictus, Haemagogus spegazzini, Culex (Culex) sp, being more positive in the points that approach the urban area surrounding Flona, among the species collected the most abundant was A. aegypti. In the Shannon trap, seventeen species were identified and the most abundant was Ochlerotatus scapularis involved in the transmission of the arboviruses Melon virus, Ilhéus virus, Rocio virus and Venezuelan Equine Encephalitis do Leste virus. Two new records were found belonging to the subfamily Culicinae: Mansonia pseudotitillans, and Culex (culex) chidesteri. The latter was first encountered with the Culex Flavivirus (CxFv) virus. CxFv has no medical importance, it is a specific virus of the Culex genus. These data show how much they still have to reveal about the Caatinga domain of Rio Grande do Norte. Conclusion: This work brings an alert for the preservation of the conservation units, because once these environments are degraded they can cause the exposure of pathogens to people's lives, since there are several vectors maintained in the environment.

6
  • CÁSSIO RICARDO DE MEDEIROS SOUZA
  • *

  • Leader : JANEUSA TRINDADE DE SOUTO
  • MEMBRES DE LA BANQUE :
  • JANEUSA TRINDADE DE SOUTO
  • LILIAN GIOTTO ZAROS DE MEDEIROS
  • CELINA MARIA PINTO GUERRA DORE
  • Data: 29 janv. 2016


  • Afficher le Résumé
  • *

7
  • TAMYRES BERNADETE DANTAS QUEIROGA
  • AVALIAÇÃO DA EXPRESSÃO DE RECEPTORES DA IMUNIDADE INATA DURANTE A INFECÇÃO EXPERIMENTAL PELO TRYPANOSOMA CRUZI UTILIZANDO CEPAS/ISOLADOS COM DIFERENTES GRAUS DE VIRULÊNCIA E PATOGENICIDADE

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • PAULO MARCOS DA MATTA GUEDES
  • ANTONIA CLAUDIA JACOME DA CAMARA
  • CLÉBER DE MESQUITA ANDRADE
  • Data: 29 janv. 2016


  • Afficher le Résumé
  • Estudos recentes têm demonstrado a importância de receptores do tipo Toll (TLRs) e do tipo NOD (NLRs) na resistência à infecção experimental pelo Trypanosoma cruzi. Entretanto, não existem trabalhos que correlacionem a expressão diferencial desses receptores em camundongos durante à infecção por cepas com diferentes graus de virulência e patogenicidade. Com a finalidade de compreender melhor o papel desses receptores na resistência ou susceptibilidade à infecção experimental pelo T. cruzi, camundongos Swiss Webster foram infectados com 1x104 tripomastigotas sanguíneos das cepas/isolados pertencentes aos seis grupos genéticos: Colombiana (Tc-I), Y (Tc-II), PL 1.10.14 (Tc-III), AM 64 (Tc-IV), 3253 (Tc-V) e CL (Tc-VI). Características biológicas inerentes as diferentes cepas/isolados foram avaliadas (parasitemia, sobrevivência e polimorfismo de formas tripomastigotas), juntamente com a expressão de receptores da imunidade inata (TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, NOD1, NOD2 e NALP3), suas moléculas adaptadoras (TRIF, MyD88, RIP2, ASC e Caspase-1), citocinas (IL-1β, IL-6, IL-10, IL-12p35, IL-12p40, IL-18, IFN-g e TNF-α) e da enzima iNOS em tecido muscular cardíaco. As cepas Y e Colombiana apresentaram os maiores picos de parasitemia e geraram 100% de mortalidade nos animais. A cepa CL e o isolado PL 1.10.14 apresentaram picos intermediários de parasitemia e geraram 70% de mortalidade nos camundongos infectados. Por outro lado, a cepa AM64 e o isolado 3253 geram os menores picos de parasitemia e 100% dos animais sobreviveram a infecção. Animais infectados com as cepas Y e Colombiana do T cruzi, cepas muito virulentas e patogênicas, apresentam elevada expressão de RNAm de NALP3, caspase-1, IL-1β, TNF-α e iNOS na musculatura cardíaca. Por outro lado, apresentam redução na expressão de RNAm de TLR4, TLR5, TLR9, TRIF, IL-6 e IL12p35 quando comparado aos demais grupos de animais infectados com cepas que apresentam menor virulência e patogenicidade. Ainda foi observado uma correlação negativa entre a expressão de RNAm de TLR4 e IL-6 e a quantidade de formas tripomastigotas sanguíneas. Os dados sugerem que as cepas Y e Colombiana, virulentas e patogênicas, induzem a inibição na expressão de RNAm de TLR4, TLR5, TLR9 e TRIF e levam ao aumento na expressão de RNAm de NALP3, caspase-1, IL-1β, TNF-α e iNOS na musculatura cardíaca podendo contribuir para a susceptibilidade a infecção.

8
  • JANETE CUNHA LIMA
  • AVALIAÇAO DA EXPRESSÃO DE RECEPTORES DA IMUNIDADE INATA EM LINHAGENS DE CAMUNDONGOS SUSCEPTÍVEIS E RESISTENTES A INFECÇÃO PELO SCHISTOSOMA MANSONI

  • Leader : PAULO MARCOS DA MATTA GUEDES
  • MEMBRES DE LA BANQUE :
  • PAULO MARCOS DA MATTA GUEDES
  • ANTONIA CLAUDIA JACOME DA CAMARA
  • CLÉBER DE MESQUITA ANDRADE
  • Data: 29 janv. 2016


  • Afficher le Résumé
  • AVALIAÇAO DA EXPRESSÃO DE RECEPTORES DA IMUNIDADE INATA EM LINHAGENS DE CAMUNDONGOS SUSCEPTÍVEIS E RESISTENTES A INFECÇÃO PELO SCHISTOSOMA MANSONI

9
  • PAULA BLANDY TISSOT BRAMBILLA
  • IMPROVEMENT OF THE VERTICAL OLFATOMETER: A NEW TOOL FOR BEHAVIORAL STUDIES OF HEMATOPHAGIC INSECTS USING AS AN EXPERIMENTAL MODEL Anopheles aquasalis (Diptera: Culicidae)

  • Leader : RENATA ANTONACI GAMA
  • MEMBRES DE LA BANQUE :
  • RENATA ANTONACI GAMA
  • PAULO MARCOS DA MATTA GUEDES
  • KELLY DA SILVA PAIXÃO
  • Data: 5 févr. 2016


  • Afficher le Résumé
  • Mosquitoes of the Anopheles genus are vectors of Plasmodium that cause human malaria, the development of new tools, methods and techniques to combat malaria transmission are a priority. In order to develop new ways of fighting the mosquito, we must understand its search behavior for the host, which is the main link in the transmission of the disease. Olfactometers are a simple and reliable way to obtain behavioral responses from insects, when used specifically for the tested insect. Widely employed in the study of agricultural entomology for pest control, however in Brazil the study with olfactometry and insect vectors is scarce. Due to the eminent importance of the development of new means to block the transmission link of pathogens from mosquitoes to humans, it is essential to optimize tools that help us to understand the behavior of the vector. Aiming this, the study had as objective the development of a vertical olfactometer with airflow for anemotaxic hematophagous insects using the species Anopheles aquasalis as a model for behavioral studies. The existence of the device was verified on the basis of worldwide public patents, later the olfactometer was developed, based on already existing olfactometers, implemented with airflow and video system, so that it would be suitable for anemotaxic insects, as described in the literature. Tests were carried out with 3 different cameras, absence and presence of airflow, smoke tests and indiscriminate bioassays with keratomone octenol and animals. In order to evaluate the functioning of the olfactometer and its implementations. As a result, we can observe that there are no specific olfactometers for A. aquasalis, nor for the genus Anopheles, and no vertical olfactometer with airflow to evaluate the olfactory response of anemotaxic insects. We observed that odor plumes generated in the water vapor smoke test are much denser, being more appropriate for evaluating the odor plume structure than those generated by acetic acid and ammonia hydroxide, in addition to being non-toxic and inexpensive . Regarding the implementations, we noticed that the GoPro Hero 3+ camera showed superior performance to the others tested, clearly assessing movements of attraction and activation of mosquitoes; the air flow generated turbulence in the odor plume tests, making diffusion active rather than passive. In the indiscriminating bioassays with octenol, we observed that rates of 15mg / h have a higher mean of attractiveness (66.66%) compared to 14mg / h (33.33%) and 18mg / h (0%), demonstrating that there is a relationship between the volatilization rate and number of insects attracted (p <0.05); with different hosts we observed that there was no relationship (p = 1.00) between different species and attractiveness of A aquasalis. It is concluded that the development of a specific tool to evaluate the behavior of anemotaxic insects that make blood-sucking insects makes the results more reliable, since the device is specific for insects, allowing the performance of simpler and more accurate behavioral studies.

2015
Thèses
1
  • SABINA DOS SANTOS PAULINO DA SILVA
  • Genes para enterotoxinas em Staphylococcus sp. isolados de manipuladores de alimentos de um restaurante universitário na cidade do Natal-RN.

  • Leader : MARIA CELESTE NUNES DE MELO
  • MEMBRES DE LA BANQUE :
  • JOSÉ SOARES DO NASCIMENTO
  • MARIA CELESTE NUNES DE MELO
  • VANIA SOUSA ANDRADE
  • Data: 24 sept. 2015


  • Afficher le Résumé
  • Os manipuladores de alimentos colonizados por Staphylococcus produtores de enterotoxinas são uma fonte potencial de intoxicação alimentar. O objetivo deste estudo foi pesquisar a presença de genes que codificam enterotoxinas em Estafilococos Coagulase Positivos (ECP) e Estafilococos Coagulase Negativos (ECN) isolados das narinas e das mãos dos manipuladores de alimentos de um restaurante universitário na cidade de Natal-RN. Trinta manipuladores de alimentos foram incluídos no estudo. O material das mãos e das narinas foi coletado utilizando um swab estéril. Os isolados foram submetidos à coloração de Gram, teste de sensibilidade a bacitracina, fermentação de manitol e provas para a catalase e coagulase livre. Os ECNs e ECPs foram posteriormente identificados através de testes bioquímicos e pelo sistema Vitek 2 (BioMerieux, França). A técnica da reação em cadeia da polimerase (PCR) foi utilizada para a detecção dos genes para as enterotoxinas A, B, C, D, E, G, H, e I (sea, seb, sec, sed, see, seg, seh, e sei) e o método de disco-difusão foi utilizado para a determinação da susceptibilidade aos antimicrobianos. Todos os manipuladores de alimentos apresentaram Estafilococos em suas mãos e/ou narinas. Foram isolados 58 Staphylococcus sp., dos quais 20,7% eram ECP e 79,3% eram ECN. Staphylococcus epidermidis foi a espécie mais prevalente. Vinte e nove Estafilococos (50%) apresentaram um ou mais genes para enterotoxinas e os genes mais prevalentes foram seg e sei, com uma frequência de 29,3% para ambos. Dentre as cepas de Staphylococcus aureus, 75% possuíam genes para enterotoxinas. Entretanto, os ECNs apresentaram uma frequência elevada de genes (43,5%). A maioria dos isolados mostrou sensibilidade aos antibióticos testados, com exceção da penicilina para a qual apenas 35% das cepas foram sensíveis. Os resultados deste estudo mostram que não somente os Estafilococos coagulase positivos, mas também os coagulase negativos são portadores de genes para enterotoxinas.

2
  • CAMILA ALVES DE OLIVEIRA
  • ASPECTOS EPIDEMIOLÓGICOS DA DENGUE NO ESTADO DA PARAÍBA NO PERÍODO DE 2011 A 2014

  • Leader : TATJANA KEESEN DE SOUZA LIMA CLEMENTE
  • MEMBRES DE LA BANQUE :
  • TATJANA KEESEN DE SOUZA LIMA CLEMENTE
  • BRUNA LEAL LIMA MACIEL
  • FABÍOLA DA CRUZ NUNES
  • Data: 23 oct. 2015


  • Afficher le Résumé
  • A dengue é uma doença infecciosa causada por um arbovírus (gênero Flavivirus, família Flaviviridae), transmitido pela picada de artrópodes, principalmente por mosquitos da espécie Aedes aegypti. Existem quatro tipos diferentes de vírus da dengue: (DENV-1, DENV-2, DENV-3 e DENV-4), que ocorre principalmente em áreas tropicais e subtropicais do mundo, inclusive no Brasil. A infecção por dengue atualmente é considerada um grave problema de saúde público mundial. Segundo a Organização Mundial de Saúde, cerca de 50-100 milhões de pessoas se infectam anualmente em mais de 100 países do mundo. O objetivo deste estudo foi descrever o perfil epidemiológico da dengue no estado da Paraíba no período entre os anos de 2011 a 2014. Uma pesquisa descritiva foi realizada, a qual utilizou dados secundários registrados no SINAN da Paraíba, onde foram analisados os casos notificados de acordo com variáveis como gênero, faixa etária, escolaridade, sorotipo, meses de notificação, classificação clínica e evolução da doença. De 2011 a 2014 na Paraíba foram notificados 53.373 casos suspeitos de dengue, dos quais 52,4% (28.020) foram confirmados. Em 2011 foram confirmados 53% (8.646) dos casos, em 2012 os confirmados foram equivalente a 58% (6.867), o ano de 2013 registrou 48% (8.827), e em 2014 com 49,5% (3.680). Houve predominância do sexo feminino (58%) dos casos. A média de idade dos confirmados foi de 18-60 anos representando 64,2%, quando considerados os anos de estudo. Quando analisado a evolução da doença, observou-se que 89% dos indivíduos confirmados com dengue evoluíram para a cura. Foram registrados 48 óbitos nesse período. O sorotipo DENV-1 foi predominante de 2011 a 2013, seguido do DENV-4. A classificação dengue clássico foi equivalente a 97% dos casos, dengue com complicações foi de apenas 0,1%, febre hemorrágica da dengue foi de 0,5% e por último a síndrome do choque da dengue. Os meses de maior notificação da doença foram no primeiro semestre do ano, com maior incidência entre os meses de março a junho. Conclui-se que as epidemias de dengue na Paraíba apresentaram diferenças importantes, e um diagnóstico imediato da infecção aliado a um diagnóstico molecular dos sorotipos circulantes na comunidade poderiam ser medidas de prevenção e controle para riscos potenciais de formas graves da doença na população.

3
  • TAINÁ COSTA BAIA
  • Atratividade de insetos às carcaças de ratos submetidos ao tratamento de etanol e flunitrazepam em uma área de Mata Atlântica no município do Natal –RN e detecção dessas substâncias por espectroscopia no infravermelho próximo.

  • Leader : RENATA ANTONACI GAMA
  • MEMBRES DE LA BANQUE :
  • RENATA ANTONACI GAMA
  • SIMÃO DIAS DE VASCONCELOS FILHO
  • VANESSA DE PAULA SOARES RACHETTI
  • Data: 29 oct. 2015


  • Afficher le Résumé
  • Estudos que demostrem a atratividade de insetos frente a carcaças tratadas com flunitrazepam e etanol são escassos na literatura. Dessa forma, o presente estudo teve como objetivo avaliar o efeito do etanol e do flunitrazepam na atratividade e diversidade de insetos em carcaças de ratos, bem como avaliar a presença dessa substância nos insetos imaturos usando a espectroscopia no infravermelho próximo (NIR) e técnicas de classificação multivariada: PCA (análise de componente principal), LDA (análise linear discriminante), SPA (algoritmo das projeções sucessivas) e GA (algoritmo genético) como ferramenta. Foram utilizados 32 ratos Wistar divididos em quatro grupos contendo oito animais em cada, sendo: grupo 1: tratado com etanol; grupo 2: flunitrazepam; grupo 3: etanol e flunitrazepam; grupo 4: água (controle). Os animais foram divididos em 32 armadilhas de cheiro colocadas em uma área de preservação ambiental, de bioma predominantemente de Mata Atlântica, no município do Natal - Rio Grande do Norte (RN), e monitoradas por cinco dias consecutivos, sendo realizadas coletadas diárias de adultos e de imaturos durante o terceiro e quarto dia. Os insetos adultos e imaturos foram identificados com auxílio de chaves dicotômicas, e os imaturos foram utilizados para a análise química através do NIR. Foram coletados 5.407 insetos, pertencentes às ordens Diptera, Coleoptera, Hymenoptera e Lepdoptera, dos quais foram identificadas 11 famílias: Calliphoridae (36,15%), Sarcophagidae (6,10%), Fannidae (6,15%), Anthomyidae (2,34%), Muscidae (1,31%), Phoridae (0,57%), Ullididae (0,24%), Drosophilidae (0,07%), Sthaphilinidae (0,01%), Formicidae (35,25%) e Vespidae (6,08%). Três novos registros de interesse forense foram feitos para o Estado do RN, sendo duas espécies da família Muscidae (Synthesiomyia nudiseta e Ophyra chalcogaster) e uma Phoridae (Megaselia scalaris). A maior atratividade de insetos foi observada para o tratamento utilizando etanol e flunitrazepam juntos (36,67% dos insetos coletados), quando comparados com o tratamento utilizando etanol (22,97%), flunitrazepam (12,87%) e ao grupo controle (27,48%). Os dados obtidos no presente estudo nos permite concluir que o tratamento etanol e flunitrazepam concomitantes provoca maior atratividade de insetos, no entanto, não há espécie e/ou padrão de sucessão entomológica exclusivo para um determinado tipo de tratamento. Através das técnicas de classificação multivariada (PCA, LDA, SPA, e GA) foram selecionadas cinco variáveis pelo SPA-LDA e dezessete pelo GA-LDA com características químicas inerente ao flunitrazem e etanol, permitindo a separação dos diferentes grupos. Portanto, o NIR e as técnicas de classificação multivariada se mostraram um método eficaz para a detecção de etanol e flunitrazepam em larvas de insetos provenientes de exames necroscópicos, permitindo ainda manter o exemplar de inseto intacto. 

SIGAA | Superintendência de Tecnologia da Informação - (84) 3342 2210 | Copyright © 2006-2024 - UFRN - sigaa08-producao.info.ufrn.br.sigaa08-producao