Banca de DEFESA: DANIELE DE SOUZA MARINHO DO NASCIMENTO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DANIELE DE SOUZA MARINHO DO NASCIMENTO
DATE: 24/09/2026
TIME: 14:00
LOCAL: REMOTA
TITLE:

BIOCHEMICAL, ANTHROPOMETRIC, AND DIETARY DETERMINANTS OF INTESTINAL PERMEABILITY: EVIDENCE FROM A SYSTEMATIC REVIEW AND AN OBSERVATIONAL STUDY – BRAZUCA-INTESTINO

KEY WORDS:

Leaky Gut, lactulose, mannitol, Multiple Source Method (MSM).

PAGES: 90
BIG AREA: Ciências da Saúde
AREA: Nutrição
SUMMARY:

The intestine is the largest interface between the human body and the external environment, being one of the first lines of defense against external pathogens, as well as being responsible for the absorption of nutrients from the diet. Several factors cause alterations in intestinal barrier permeability (IBP), such as physical exercise, medication use, and nutritional status. This thesis aimed to determine whether anthropometric and biochemical variables and usual food intake are associated with intestinal epithelial barrier function. Two sub-studies were conducted; the first aimed to perform a systematic review evaluating whether diet can alter IBP in healthy individuals. The review was prepared according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols (PRISMA-P) guidelines, and its protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO: CRD 42023400381) database. Search strategies were developed based on a combination of descriptors and executed in the PubMed, Embase, Scopus, Web of Science, and CINAHL databases. Observational and intervention studies that linked diet or dietary intervention to IBP in healthy individuals were included. The methodological quality of the studies was assessed using the adapted Newcastle-Ottawa scale for observational studies and the Cochrane risk-of-bias tool (RoB 2) for intervention studies. The Grading of recommendations assessment, development, and evaluation (GRADE) framework was used to assess the quality of the evidence. Based on the results obtained, we can conclude that physiological immaturity in early life is responsible for the intestinal permeability observed in infants, regardless of the type of milk received, and it is important that complementary foods are introduced only after 12 weeks of age. Probiotics and inulin were positively associated with a reduction in intestinal barrier permeability. The second sub-study aimed to evaluate whether there is an association and/or correlation between IBP and anthropometric and biochemical variables and usual food intake. It was a cross-sectional study, with data collection carried out in two periods, from October 2019 to March 2020 and from November 2021 to March 2023. One hundred and one people participated, and anthropometric, socioeconomic, lifestyle, self-reported disease, and biological data (blood and urine) were collected. IBP was assessed using the lactulose/mannitol oral intake test, and usual food intake was estimated through dietary analysis of food consumption using a 24-hour recall. The Multiple Source Method (MSM) was used to assess individual usual food intake of each nutrient, through the generated residues, adjusted for intrapersonal variability and without the influence of total energy consumed. In the studied population, individuals with L/M ≥P50 presented lower habitual intake of total, saturated, and monounsaturated fats, while magnesium and potassium intake were higher. Correlations indicated that reduced serum retinol levels, smaller hip circumference, and lower intake of total and saturated fats were associated with increased intestinal permeability, measured L/M ratio. The regression model revealed that higher serum retinol concentrations (AOR = 0.538; 95% CI = 0.319–0.910; p = 0.021) and higher intake of monounsaturated fats (AOR = 0.855; 95% CI = 0.749–0.975; p = 0.019) were associated with lower intestinal barrier permeability. In light of the above, it can be concluded that a larger hip circumference, higher serum retinol levels, and higher consumption of fatty acids, especially monounsaturated, are inversely related to increased intestinal barrier permeability. Although we have found these preliminary results, there are still gaps to be filled regarding usual dietary intake and its impact on intestinal permeability, and this study was among the pioneers to have this design, focused on the dietary reality of people responsible for their own daily food choices.

COMMITTEE MEMBERS:
Externa à Instituição - LIGIANA PIRES CORONA - UNICAMP
Presidente - 2646462 - BRUNA LEAL LIMA MACIEL
Externa à Instituição - DEIZIANE VIANA DA SILVA COSTA - UVir
Externa ao Programa - 2306763 - KARINE CAVALCANTI MAURICIO DE SENA EVANGELISTA - nullExterna ao Programa - 1452705 - SEVERINA CARLA VIEIRA CUNHA LIMA - null