Banca de QUALIFICAÇÃO: RAFAELA ALVES DE LIMA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : RAFAELA ALVES DE LIMA
DATE: 05/03/2024
TIME: 14:00
LOCAL: videoconferencia - https://meet.google.com/wyw-nzqp-pxy
TITLE:

Bioinformatics analysis of oxidative stress in plants under microgravity and its comparison with hypoxia and heat

KEY WORDS:

Abiotic stress. Change in gravitational force. Redox homeostasis. Metabolic pathway.

PAGES: 125
BIG AREA: Ciências Biológicas
AREA: Bioquímica
SUBÁREA: Biologia Molecular
SUMMARY:

Plants frequently encounter environmental changes, and their responses are associated to oxidative stress. Microgravity, hypoxia, and heat are examples of abiotic stressors that generate an oxidative imbalance. Decreased gravity is an environmental alteration that also induces oxidative stress. However, there is still a lack of consensus regarding all the effects induced by this condition. Comparing microgravity studies with other environmental factors can enhance the understanding of plant responses to microgravity. In this context, transcriptomic data integration from studies published on open platforms about microgravity, hypoxia, and thermal stress in plants was conducted to deepen the understanding of the response to microgravity and explore the evolution of its effects under different exposure times. Bioinformatics tools were employed to identify target genes, ontologies, and significantly activated metabolic pathways. Integrating data from 18 microgravity studies with 10 heat studies and 10 hypoxia studies revealed significant downregulation of genes related to chlorophyll biosynthesis and processes associated with light-harvesting proteins. This suggests that photooxidation may have a pronounced effect under microgravity conditions. This effect appears to diminish with increasing exposure time. There was also overlap in the activation of antioxidant metabolic pathways: glutathione metabolism, phenylpropanoid biosynthesis, and phytohormone transduction, demonstrating that plants seek redox homeostasis through different strategies. The antioxidant effect persists throughout the exposure time, being stronger in the initial days of exposure. Target genes were also identified, with most related to chlorophyll production and production of propanoids. Thus, the plant's response to microgravity-induced oxidative stress seems to significantly impair photosynthesis, but this effect is accompanied by robust antioxidant activity. In summary, this research explores the plant's response to microgravity-induced oxidative stress and its implications for photosynthesis. Robust antioxidant mechanisms are activated to maintain redox balance despite the challenges imposed by microgravity. By comparing these responses to other environmental stresses, valuable insights are gained into how plants adapt to space conditions. This knowledge has implications for enhancing crop resilience in space agriculture.

COMMITTEE MEMBERS:
Presidente - 1880243 - DANIEL CARLOS FERREIRA LANZA
Interno - 1046922 - LEONARDO CAPISTRANO FERREIRA
Externa ao Programa - ***.423.414-** - GÉSSICA LAIZE BERTO GOMES - INSA