Banca de QUALIFICAÇÃO: LIZIANE VIRGINIA PEREIRA FREIRE

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : LIZIANE VIRGINIA PEREIRA FREIRE
DATE: 29/08/2025
TIME: 14:00
LOCAL: Salas da GEP/MEJC
TITLE:

EVALUATION OF GENOMIC INSTABILITY AND CELL CYCLE IN WOMEN WITH ENDOMETRIOSIS

KEY WORDS:

Endometriosis; Genomic instability; Cell cycle; Infertility; Biomarkers

PAGES: 50
BIG AREA: Ciências da Saúde
AREA: Saúde Coletiva
SUMMARY:

Endometriosis is a chronic, multifactorial inflammatory gynecological disease that affects approximately 10-15% of women of reproductive age and is associated with chronic pelvic pain, infertility, and impaired quality of life. Recent evidence suggests that genomic instability and cell cycle dysregulation play a central role in the pathophysiology of the disease. This study aimed to evaluate the frequency of biomarkers of genomic instability (apoptosis, micronuclei, nucleoplasmic bridges, and nuclear buds) and cell cycle alterations in women with endometriosis, relating them to clinical variables such as disease severity, fertility, progestin use, and the presence of chronic noncommunicable diseases (NCDs). This is an observational, cross-sectional study conducted with 55 women diagnosed with endometriosis. Clinical and sociodemographic questionnaires were administered, in addition to peripheral blood collection for the cytokinesis-blocked micronucleus assay (CBMN) and analysis of cell cycle phases (G1, S, and G2) by flow cytometry. A significant correlation was observed between the degree of endometriosis and infertility (ρ = –0.41; p = 0.0038), demonstrating that more advanced stages are associated with lower fertility. Although most biomarkers evaluated did not show statistically significant differences between the groups, it was found that women with NCDs (p = 0.0110) and infertile women (p = 0.0203) had a lower percentage of cells in the G2 phase, suggesting a failure in the G2/M checkpoint. Other trends were also observed, such as the potentially protective effect of progestin, associated with a higher frequency of apoptosis and lower proliferative activity with its use, as well as a progressive reduction in the G2 phase as the disease worsened, although both findings did not reach statistical significance (p > 0.05). It is concluded that genomic instability and cell cycle dysregulation may be implicated in the pathophysiology of endometriosis and its association with infertility and NCDs. Despite the lack of statistical significance, the preliminary data indicate relevant trends that justify expanding the sample to confirm the findings and develop non-invasive diagnostic strategies.

COMMITTEE MEMBERS:
Presidente - 3313589 - JANAINA CRISTIANA DE OLIVEIRA CRISPIM FREITAS
Externa à Instituição - PAULA ANDREA DE ALBUQUERQUE SALLES NAVARRO - USP
Interna - 1674709 - VIVIANE SOUZA DO AMARAL