Banca de DEFESA: MARIA CAROLINA DANTAS CAMPELO NEVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIA CAROLINA DANTAS CAMPELO NEVES
DATE: 25/10/2024
TIME: 14:00
LOCAL: Sala de Aulas II GEP/MEJC
TITLE:

PROFILE OF CELL DEATH IN WOMEN WITH DIABETES TYPE 1, 2 AND GESTATIONAL DIABETES

KEY WORDS:

Gestational diabetes; Annexins; Flow Cytometry; Immunological Tests.

PAGES: 90
BIG AREA: Ciências da Saúde
AREA: Enfermagem
SUMMARY:

Introduction: The gestational period is marked by physiological changes that seek to meet the demands of mother-baby interaction for the balanced development of the baby. A pregnancy accompanied by Diabetes Mellitus enhances oxidative stress mediated by the inflammatory response that culminates in the production of reactive oxygen and nitrogen species, generating a maternal and placental imbalance characterized by damaging molecules such as DNA and RNA. Therefore, the impact caused by Diabetes Mellitus on cells and its outcomes have been investigated in pregnant women. Objective: To analyze the cellular profile and characteristics together with the inflammatory process of Diabetes Mellitus during pregnancy in a teaching maternity hospital. Methodology: This is an observational and descriptive study, with a quantitative approach, operationalized in two stages: cross-sectional study and case-control study. 240 diabetic and 12 non-diabetic pregnant women were characterized sociodemographically. Of the 240 diabetic pregnant women, 23 were analyzed for the degree of apoptosis in the lymphocyte, monocyte and granulocyte populations of whole blood, incubated with the fluorochromes annexin V FIT-C and/or propidium iodide and characterized by Cytoflex flow cytometry (Beckman Coulter) for detection of apoptosis. Results: Of the population studied, 46.25% were between 30 and 39 years old, 57.08% were brown, 50% had a salary income ≤ 01 minimum wage, 57.08% had high school education, 62.92% lived in rural areas; 65.83% had sanitation, 97.92% had electricity and 71.25% lived on paved streets. 67.74% did not notice symptoms of diabetes before diagnosis, 77.41% were diagnosed during pregnancy, 57.14% had a family history of diabetes and 67.86% had a family history of hypertension. Of the 240 pregnant women, 79.17% had GDM, 5.83% had DM1 and 15.00% had DM2. In addition, 40.42% had hypertension and 19.58% reported having other associated comorbidities. The characterization of the cellular profile in the study population showed a greater quantity of live cells in the distribution of lymphocytes and granulocytes between the control population and women with GDM (p=0.001/p=0.04) and with DM1/DM2 (p=0.04). Also in the lymphocyte population, early apoptosis showed a greater statistical difference than late apoptosis. Necrosis showed a statistically significant difference between the control group and the group of women with GDM (p=0.03). Monocytes did not show a statistically significant difference between the groups regarding live cells in early and late apoptosis. The diabetic participants whose blood was analyzed were between the 12th and 38th gestational week and apoptosis was present in all of them. Final considerations: In general, there was a greater quantity of live cells in the control population when compared to the groups of pregnant women with diabetes in the lymphocyte and granulocyte population. In addition, the difference in the expression of apoptosis between the groups of women with GDM only and the group of women with type 1 and 2 diabetes was minimal. Therefore, regardless of the type of diabetes, the pregnant woman suffered cell loss throughout the pregnancy.

COMMITTEE MEMBERS:
Externa ao Programa - 3221432 - CAROLINE EVELIN NASCIMENTO KLUCZYNIK - nullPresidente - 1714243 - DANIELLA REGINA ARANTES MARTINS SALHA
Externa à Instituição - MARIA OLIVIA AMADO RAMOS BACELLAR - UFBA