Evaluation of lytic polysaccharide monooxygenase production from Trichoderma reesei with pretreated coconut fiber
green coconut fiber, cellulosic ethanol, enzymatic hydrolysis, LPMOs.
: Incorrect disposal of green coconut residuo can lead to serious environmental and public health problems, due to its accumulation and slow decomposition. Green coconut fiber is a lignocellulosic material, ie composed mainly of cellulose, lignin and hemicellulose, which has great potential for production of cellulosic ethanol. In this process, the raw material is hydrolyzed into fermentable sugars for later action of microorganisms for ethanol production. The biochemical route is an attractive and sustainable alternative, but the high costs of cellulolytic enzymes production is one of the main limiting factors. Therefore, as an alternative, the use of biomass itself for the enzymatic cocktails production has proposed. In addition, studies have highlighted the action of accessory enzymes, such as lytic polysaccharide monooxygenases (LPMOs), in increasing cellulose hydrolysis performance. The action of LPMOs consists in the formation of new reducing and non-reducing terminals, acting on crystalline cellulose structures. The AA9 family enzymes are produced exclusively by fungi and the synergy between them and the cellulases can improve the performance of enzymatic hydrolysis of the lignocellulosic biomass. Thus, the present work aims to evaluate lignocellulolytic enzymes and LPMOs production from Trichoderma reesei using natural to steam and alkali pretreated green coconut fiber.