Production of lipid oils by Rhodosporidium toruloides using sugarcane molasses as a substrate aiming at obtaining biodiesel.
Biodiesel; Rhodosporidium toruloides; sugarcane molasses; lipases
The growing demand for fuels and the environmental impacts associated with the use of fossil sources have driven the search for sustainable alternatives, among which third-generation biofuels stand out. These fuels, produced from oleaginous microorganisms, represent a promising solution as they do not compete with food production and enable the use of agro-industrial residues. In this context, the present study aims to evaluate the production of third-generation biodiesel from microbial oil obtained from the yeast Rhodosporidium toruloides CCT 0783 cultivated in sugarcane molasses, an abundant and low-cost by-product of the sugar-energy industry. The study will involve the physicochemical characterization of molasses, the cultivation of the yeast, and the extraction of microbial oil, followed by the production of biodiesel through two distinct routes: a chemical route using an alkaline catalyst, and an enzymatic route using the lipases CALB (Candida antarctica Lipase B) and Eversa® Transform 2.0. The obtained samples will be analyzed for acidity index and ester profile by gas chromatography, allowing the comparison of yield and quality between the processes. The expected results aim to demonstrate the technical and environmental feasibility of using agro-industrial residues in renewable energy generation, contributing to the advancement of biotechnology and the development of energy-efficient and sustainable alternatives.