Banca de DEFESA: ANA RAQUEL MELO DE FARIAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANA RAQUEL MELO DE FARIAS
DATA : 28/11/2018
HORA: 10:00
LOCAL: INSTITUTO DO CEREBRO
TÍTULO:

 REPROGRAMMING OF ASTROGLIAL CELLS INTO NEURONS USING A COCKTAIL OF SMALL MOLECULES

PALAVRAS-CHAVES:

neuronal differentiation; cell reprogramming; astrocytes; induced neurons; small molecules;

PÁGINAS: 65
GRANDE ÁREA: Ciências Biológicas
ÁREA: Fisiologia
RESUMO:

The reprogramming of different specialized cell types in others has been a field widely studied in recent years. More specifically, the generation of induced neurons (iNs) is used with the objective of applying these cells in the study of pathological models and in cellular therapy, focusing on the treatment of patients with neurodegenerative diseases or acute lesions in the central nervous system. Several methodologies have been used for this approach, including the reprogramming of cells from other lineages into neurons, either directly or indirectly. However, most of the cellular reprogramming protocols depend on the overexpression of ectopic genes, which may lead to other transient changes not desired in reprogrammed cells. In order to overcome these possible side effects, we investigated the possibility of reprogramming astrocytes from postnatal mice into neurons through the transient exposure of the cells to a cocktail of small molecules added to the cell culture medium. This cocktail includes molecules that act in different cellular pathways, among which the regulation of gene expression, the modulation of neurogenesis and the control of the cell cycle, previously been used in the reprogramming of fibroblasts into neurons. To demonstrate the phenotype of the cells after treatment with the cocktail, aspects such as the expression of typically neuronal and astrocytic proteins, morphology, survival, proliferation and gene expression were evaluated through the techniques of immunocytochemistry, time-lapse video-microscopy and RT-qPCR. Based on the data obtained it was observed that the drug cocktail used induced in the treated astrocytes an increase in the expression of genes related to the neuronal profile and a significant change in its morphology, although this is not typical of neurons. Thus indicating that the combination of small molecules used is not sufficient to effectively reprogram astrocyte cells into induced neurons.

MEMBROS DA BANCA:
Externo à Instituição - CECILIA HEDIN PEREIRA - UFRJ
Interno - 1976236 - EMELIE KATARINA SVAHN LEAO
Presidente - 1674643 - MARCOS ROMUALDO COSTA