Banca de DEFESA: BRENNA MARCELIANE DE MELO MARCELINO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : BRENNA MARCELIANE DE MELO MARCELINO
DATE: 22/01/2021
TIME: 14:00
LOCAL: Modo Remoto
TITLE:

Role of the complement system in the pathogenesis of severe malaria - an in vitro model approach

KEY WORDS:

Malaria; Plasmodium falciparum; Complement System

PAGES: 74
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUBÁREA: Protozoologia de Parasitos
SPECIALTY: Protozoologia Parasitária Humana
SUMMARY:

Malaria is a parasitic infectious disease, caused by protozoa of the genus Plasmodium and characterized by acute fever, chills, sweating and headache. six species cause human malaria worldwide: P. falciparum, P. vivax, P. malariae, P. ovale and P. knowlesi and P. simium. Most cases of severe human malaria are attributed to infections by P. falciparum, considered the most aggressive due to tissue hypoxia resulting from several factors, including severe anemia. According to the 2017 World Malaria Report, there were about 219 million cases of malaria and 435,000 deaths from the disease each year. As an intracellular parasite, Plasmodium depends on the host to survive and genetically determined factors can influence the individual's susceptibility to clinically developing malaria, especially those that result in hematological changes. Thus, the present study aims to evaluate the participation of the complement system in the genesis of the phenomenon of hemolysis induced by P. falciparum, understanding of the mechanism of hemolysis of human erythrocytes treated with total proteins of P. falciparum mediated by the complement system. For this, the methodology comprises the in vitro cultivation of the W2 strain of P. falciparum, in addition to animal experimentation using Swiss mice immunized with protein extract from the culture of P. falciparum for the production of polyclonal serum, in addition to autologous hemolytic assays and determination of complement fragments by flow cytometry. The protein profiles visualized through polyacrylamide gel electrophoresis (SDS-PAGE), allows the identification of soluble P. falciparum proteins. The immunization of the animals with processed extracts of P. falciparum enabled the production of functional murine anti-P. falciparum antibodies.  that can even be used as a polyclonal serum.

BANKING MEMBERS:
Interno - 2213126 - VALTER FERREIRA DE ANDRADE NETO
Externo ao Programa - 2275890 - MARCELO DE SOUSA DA SILVA
Externa ao Programa - 350753 - VALERIA SORAYA DE FARIAS SALES
Externa à Instituição - YORLEYDY RUIZ MORENO