Banca de DEFESA: MARÍLIA VIRGO SILVA ALMEIDA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARÍLIA VIRGO SILVA ALMEIDA
DATE: 21/02/2020
TIME: 09:00
LOCAL: Sala de aula do PPGCB/PPGBP
TITLE:

EVALUATION OF INTESTINAL ANTI-INFLAMMATORY POTENTIAL OF LACTOBACILLUS PLANTARUM CNPC003 AND LACTOBACILLUS MUCOSAE CNPC007 PROBIOTICS IN MODEL OF DNBS-INDUCED INTESTINAL INFLAMMATION

KEY WORDS:

Intestinal inflammation; probiotics strains; sulfasalazine; inflammatory markers; oxidative stress

PAGES: 85
BIG AREA: Ciências Biológicas
AREA: Farmacologia
SUMMARY:

Inflammatory Bowel Disease (IBD), characterized by presenting an uncontrolled chronic inflammation in the intestinal mucosa, mainly comprises two pathologies: Ulcerative Colitis (UC) and Crohn's Disease (CD). In these, the local immune system remains chronically activated and the intestine chronically inflamed, due to an inability to decrease inflammatory responses. The production and release of reactive oxygen species (ROS) and pro-inflammatory cytokines by phagocytes play an important role in the pathophysiology of IBD. Therapeutic failures and adverse effects of the drug arsenal of choice have encouraged researchers to study alternatives in the control of the intestinal inflammatory process, among which are probiotic bacteria. Thus, this research aimed to evaluate the intestinal anti-inflammatory activity of the pretreatment with the probiotics Lactobacillus plantarum CNPC003 (LP) and Lactobacillus mucosae CNPC007 (LM) in a model of acute experimental colitis induced by dinitrobenzenesulfonic acid (DNBS) in rats. Thus, female Wistar rats (225-245 g) were used, divided into 5 experimental groups (n = 7): Healthy group: Healthy (S) that received saline solution; Collitic groups: DNBS control (DNBS) that received saline solution; and the colitics treated with probiotics: LP, LM and standard drug sulfasalazine (SSZ) at a dose of 250 mg / kg. The respective pretreatments for each group were administered by gavage for 17 days before induction of intestinal inflammation. After treatments, on the 17th day, colitis was induced by the intracolonic administration of 30 mg of DNBS (50% ethanol, v / v) and then the administrations were continued until the 19th day. On the 20th day, seventy-two hours after induction, all animals were euthanized. Macroscopic, microscopic parameters, inflammatory markers such as myeloperoxidase and cytokines tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), interleukin-β (IL-1β), oxidative stress (malondialdehyde) were evaluated and the integrity of the mucin-2 (MUC-2) and ocludine (OCL) intestinal barrier. Pre-treatment with probiotics was able to attenuate the severity of the colonic damage caused by DNBS, observed in the reduction of the macroscopic damage score (p <0.05 vs. DNBS). This effect was associated with a significant reduction in myeloperoxidase (MPO) activity (p <0.05 vs. DNBS) and in the levels of pro-inflammatory cytokines TNF-α and IL-1β (p <0.05 vs. DNBS) . On the other hand, probiotics increased the colonic levels of IL-10, well known as an anti-inflammatory cytokine. The beneficial effect of probiotics on intestinal inflammation induced by DNBS was also demonstrated by the ability to significantly reduce colonic oxidative stress, observed by the reduction of malondialdehyde (MDA) (p <0.05 vs. DNBS). Additionally, LP and LM increased the expression of markers involved in epithelial integrity such as OCL and MUC-2. Thus, the probiotics LM and LP stand out for presenting an intestinal anti-inflammatory potential that could be an alternative in the prevention of IBD.

 

 

BANKING MEMBERS:
Presidente - 2330188 - GERLANE COELHO BERNARDO GUERRA
Interno - 2412258 - EDILSON DANTAS DA SILVA JUNIOR
Externo à Instituição - RITA DE CÁSSIA RAMOS DO EGYPTO QUEIROGA - UFPB