Banca de DEFESA: BRUNO DE PAIVA RÊGO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : BRUNO DE PAIVA RÊGO
DATA : 27/08/2019
HORA: 14:30
LOCAL: sala SS1 - Bioquímica
TÍTULO:

GEOGRAPHICAL DISTRIBUTION, MORPHOLOGY AND MOLECULAR PHILOGENY OF Coleodactylus natalensis FREIRE, 1999 (SQUAMATA, SPHAERODACTYLIDAE)

PALAVRAS-CHAVES:

Genetic variability; Sphaerodactylinae; Atlantic Forest; lizards; species distribution model; genetics of populations; DNA sequencing; haplotypes.

PÁGINAS: 80
GRANDE ÁREA: Ciências Biológicas
ÁREA: Genética
SUBÁREA: Genética Animal
RESUMO:

Evolutionary processes are multidimensional and usually more evident in isolated areas, rendering sympatric species susceptible to greater intrapopulation variation. Following this model, the five lizard species of the genus Coleodactylus occur only in forested areas of South America, and three of these in Northeastern Brazil, constituting the meridionalis group. In this clade, C. natalensis diverged 1.9 million years ago from C. meridionalis, maintaining high overlapping meristic characters. The latter inhabits forest remnants throughout Northeastern Brazil, and can inhabit Atlantic Forest sites where C. natalensis is endemic in the state of Rio Grande do Norte (RN). In view of the restricted occurrence of C. natalensis, this work investigated potential areas of occupation, through species distribution modeling; in addition, it aimed to characterize the specimens morphologically and genetically, as well as to identify the existence of sympatry in these populations and the retention of molecular identities in this possible condition. For this, the populations of the genus were collected in Natal Dune State Park (PEDN), Municipal Park Dom Nivaldo Monte (PCNM), Environmental Protection Area of Jenipabu (APAJ) and Mata da Pipa State Park (PEMP). In the morphological analyzes, individuals deposited in the Herpetological Collection of UFRN were also included. For the construction of bioinformatics data such as haplotype networks and phylogenetic reconstructions, regions of the mitochondrial genes cytb and 16S, and nuclear genes c-mos, RAG-1 were sequenced. The species distribution model showed a restricted occurrence for C. natalensis, influenced by a small area of environmental suitability. Twenty five specimens of Coleodactylus spp. were collected, which meristically formed two groups: one composed of the populations of APAJ, PEDN, PCNM and PEMP, and another with PEMP specimens only. Molecular analyzes corroborated the results found for morphology, with the same groupings mainly answered by cytb and 16S haplotype networks. The RAG-1 and c-mos genes were very conservative in their sequences, linking individuals from distant locations in their networks. In phylogenetic reconstructions, it is possible to identify two strains of C. natalensis for three sampled areas. PEMP specimens are the most distinct in the nucleotide sequences and sister group of the other populations; PCNM forms a clade with APAJ and with only one PEMP individual. In general, the generated model confirms that C. natalensis is a species with occurrence restricted to the Atlantic Forest remnants of the state of Rio Grande do Norte. Additionally, the analysis of the morphological and molecular results of this study suggests the existence of three subspecies composing the referred taxon.

MEMBROS DA BANCA:
Presidente - 1453487 - KATIA CASTANHO SCORTECCI
Externa ao Programa - 1121066 - ELIZA MARIA XAVIER FREIRE
Externo ao Programa - 1199139 - WAGNER FRANCO MOLINA
Externa à Instituição - KATIA CRISTINA MACHADO PELLEGRINO - UNIFESP