Banca de QUALIFICAÇÃO: FELIPE DE LIMA TORRES

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : FELIPE DE LIMA TORRES
DATA : 16/05/2019
HORA: 09:00
LOCAL: Anfiteatro das Aves
TÍTULO:

EVALUATION OF SEROTONIN IMMUNORRERATIVE CELLS IN THE LATERAL AND VENTRAL REGIONS OF THE DORSAL NUCLEUS OF RAPHE IN RATS AFTER CONTINUOUS USE AND WITHDRAWAL OF ALCOHOL

PALAVRAS-CHAVES:

Alcohol. Dorsal nucleus of raphe. Serotoninergic system

PÁGINAS: 50
GRANDE ÁREA: Ciências Biológicas
ÁREA: Farmacologia
SUBÁREA: Neuropsicofarmacologia
RESUMO:

The abusive consumption of ethyl alcohol has become one of the major problems of public health worldwide, mainly due to the high prevalence of cormobidades, such as depression and anxiety, in individuals with a dependence history. Alcohol influences the activity of the serotonergic neurons in the dorsal nucleus of the raphe leading to neuroadaptive changes involved in all stages of dependence, from the beginning of the consumption to the withdrawal and relapse. Considering the higher susceptibility of female gender to the effects of alcohol, the present study aimed to investigate if the continuous consumption of alcohol, followed by its short or long-term withdrawal can cause changes in the density of immunoreactive cells to serotonin (5-HT) in the lateral and ventral regions of the rat dorsal raphe nucleus. The animals were divided into four groups according to pre-established consumption and withdrawal protocols. Excepting control group, which received only water as a source of liquid diet, all animals were submitted to alcohol treatment in increasing concentrations (2%, 4% and 6%). All animals were submitted to transcardiac perfusion for brain removal and subsequent immunohistochemical analysis. The imonohistochemistry analysis for 5-HT was performed in the lateral (DRL) and ventral (DRV) regions of the dorsal nucleus of rafe using the Atlas of the Estereotactic Atlas of Rat, optical microscope coupled to the digital camera and Neurolucida software. The analyzed areas were subdivided into caudal, medial and rostral portions. In order to compare the distribution of number and cell density between treatments, the data set was submitted to the analysis of variance (ANOVA unifatorial) followed by the Tukey test when necessary (values of p≤0.05 were considered statistically significant). We considered the isolated analysis of each subdivision, as well as the analysis of all the portions together. In the DRL area, the cell density was increased in the long-term withdrawal group compared to the control only when all subdivions were considered. A significant increase in cell density in the medial portion was observed in DRV for all groups when compared to control. In the same area, an increase in density was also observed in the short-term group in the caudal portion when compared to the control. An increase in cell density was also observed in in DRV area considering all portions for all experimental groups compared sto the control. No significant changes in the number of cells marked for 5-HTwas observed in the areas of interest (DRL and DRV). The results suggest a possible involvement of the DRL and DRV serotonergic cell groups in alcohol-related disorders, in view of the functional aspects of these areas.

MEMBROS DA BANCA:
Presidente - 2412258 - EDILSON DANTAS DA SILVA JUNIOR
Externo ao Programa - 3550124 - JUDNEY CLEY CAVALCANTE
Externa ao Programa - 2140860 - ROVENA CLARA GALVAO JANUARIO ENGELBERTH